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Special Issue "Advances on Bacterial Genomics"

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 31 March 2021.

Special Issue Editors

Prof. Dr. Anne M. Alvarez
Website1 Website2
Guest Editor
University of Hawaii at Manoa, Honolulu, United States
Interests: bacterial classification and genomics; population diversity; serological and molecular diagnostics; epidemiology
Dr. Mohammad Arif
Website1 Website2
Guest Editor
University of Hawaii at Manoa, Honolulu, United States
Interests: bacterial genomics and evolution; microbiome; molecular plant-bacterial interactions; Molecular diagnostics

Special Issue Information

Dear Colleagues,

Genome sequence-based investigations are emerging as a key area in bacterial research. Bacterial interactions with the host—and infection severity—depend on genome constituents. There has been a tremendous increase in bacterial genome-wide analyses. In this Special Issue, we intend to highlight the genome-wide analyses of pathogenicity determinants, CRISPR-Cas, type secretion systems, antimicrobial, EPS, and other important areas of genomics. Our aim is to provide a collection of manuscripts that describe the genomic biology and analyses of different bacterial pathogens. Research articles and reviews are invited.

Submissions on—but not limited to—the following topics are invited:

  • Genome-wide comparative genomics;
  • Bacterial phylogenomics, evolution, and speciation;
  • Functional genomics;
  • Analyses of pathogenicity determinants and CRISPR cas;
  • Genome-informed molecular diagnostics.

Prof. Dr. Anne M. Alvarez
Dr. Mohammad Arif
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Comparative genomics
  • Genomic evolution
  • Phylogenomics
  • Pathogenicity determinanats
  • CRISPR cas
  • Type secretion systems
  • Bacterial speciation
  • Functional genomics
  • Genome-informaed diagnostics

Published Papers (3 papers)

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Research

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Open AccessArticle
Growth by Insertion: The Family of Bacterial DDxP Proteins
Int. J. Mol. Sci. 2020, 21(23), 9184; https://doi.org/10.3390/ijms21239184 - 02 Dec 2020
Abstract
We have identified a variety of proteins in species of the Legionella, Aeromonas, Pseudomonas, Vibrio, Nitrosomonas, Nitrosospira, Variovorax, Halomonas, and Rhizobia genera, which feature repetitive modules of different length and composition, invariably ending at the COOH side with Asp–Asp–x–Pro (DDxP) motifs. DDxP proteins [...] Read more.
We have identified a variety of proteins in species of the Legionella, Aeromonas, Pseudomonas, Vibrio, Nitrosomonas, Nitrosospira, Variovorax, Halomonas, and Rhizobia genera, which feature repetitive modules of different length and composition, invariably ending at the COOH side with Asp–Asp–x–Pro (DDxP) motifs. DDxP proteins range in size from 900 to 6200 aa (amino acids), and contain 1 to 5 different module types, present in one or multiple copies. We hypothesize that DDxP proteins were modeled by the action of specific endonucleases inserting DNA segments into genes encoding DDxP motifs. Target site duplications (TSDs) formed upon repair of staggered ends generated by endonuclease cleavage would explain the DDxP motifs at repeat ends. TSDs acted eventually as targets for the insertion of more modules of the same or different types. Repeat clusters plausibly resulted from amplification of both repeat and flanking TSDs. The proposed growth shown by the insertion model is supported by the identification of homologous proteins lacking repeats in Pseudomonas and Rhizobium. The 85 DDxP repeats identified in this work vary in length, and can be sorted into short (136–215 aa) and long (243–304 aa) types. Conserved Asp–Gly–Asp–Gly–Asp motifs are located 11–19 aa from the terminal DDxP motifs in all repeats, and far upstream in most long repeats. Full article
(This article belongs to the Special Issue Advances on Bacterial Genomics)
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Open AccessArticle
An Earliest Endosymbiont, Wolbachia massiliensis sp. nov., Strain PL13 from the Bed Bug (Cimex hemipterus), Type Strain of a New Supergroup T
Int. J. Mol. Sci. 2020, 21(21), 8064; https://doi.org/10.3390/ijms21218064 - 29 Oct 2020
Abstract
The symbiotic Wolbachia are the most sophisticated mutualistic bacterium among all insect-associated microbiota. Wolbachia-insect relationship fluctuates from the simple facultative/parasitic to an obligate nutritional-mutualistic association as it was the case of the bedbug-Wolbachia from Cimexlectularius. Understanding this association may [...] Read more.
The symbiotic Wolbachia are the most sophisticated mutualistic bacterium among all insect-associated microbiota. Wolbachia-insect relationship fluctuates from the simple facultative/parasitic to an obligate nutritional-mutualistic association as it was the case of the bedbug-Wolbachia from Cimexlectularius. Understanding this association may help in the control of associated arthropods. Genomic data have proven to be reliable tools in resolving some aspects of these symbiotic associations. Although, Wolbachia appear to be fastidious or uncultivated bacteria which strongly limited their study. Here we proposed Drosophila S2 cell line for the isolation and culture model to study Wolbachia strains. We therefore isolated and characterized a novel Wolbachia strain associated with the bedbug Cimexhemipterus, designated as wChem strain PL13, and proposed Wolbachiamassiliensis sp. nov. strain wChem-PL13 a type strain of this new species from new supergroup T. Phylogenetically, T-supergroup was close to F and S-supergroups from insects and D-supergroup from filarial nematodes. We determined the 1,291,339-bp genome of wChem-PL13, which was the smallest insect-associated Wolbachia genomes. Overall, the wChem genome shared 50% of protein coding genes with the other insect-associated facultative Wolbachia strains. These findings highlight the diversity of Wolbachia genotypes as well as the Wolbachia-host relationship among Cimicinae subfamily. The wChem provides folate and riboflavin vitamins on which the host depends, while the bacteria had a limited translation mechanism suggesting its strong dependence to its hosts. However, the clear-cut distinction between mutualism and parasitism of the wChem in C. hemipterus cannot be yet ruled out. Full article
(This article belongs to the Special Issue Advances on Bacterial Genomics)
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Review

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Open AccessReview
Brucella Genomics: Macro and Micro Evolution
Int. J. Mol. Sci. 2020, 21(20), 7749; https://doi.org/10.3390/ijms21207749 - 20 Oct 2020
Abstract
Brucella organisms are responsible for one of the most widespread bacterial zoonoses, named brucellosis. The disease affects several species of animals, including humans. One of the most intriguing aspects of the brucellae is that the various species show a ~97% similarity at the [...] Read more.
Brucella organisms are responsible for one of the most widespread bacterial zoonoses, named brucellosis. The disease affects several species of animals, including humans. One of the most intriguing aspects of the brucellae is that the various species show a ~97% similarity at the genome level. Still, the distinct Brucella species display different host preferences, zoonotic risk, and virulence. After 133 years of research, there are many aspects of the Brucella biology that remain poorly understood, such as host adaptation and virulence mechanisms. A strategy to understand these characteristics focuses on the relationship between the genomic diversity and host preference of the various Brucella species. Pseudogenization, genome reduction, single nucleotide polymorphism variation, number of tandem repeats, and mobile genetic elements are unveiled markers for host adaptation and virulence. Understanding the mechanisms of genome variability in the Brucella genus is relevant to comprehend the emergence of pathogens. Full article
(This article belongs to the Special Issue Advances on Bacterial Genomics)
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