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Article

An Earliest Endosymbiont, Wolbachia massiliensis sp. nov., Strain PL13 from the Bed Bug (Cimex hemipterus), Type Strain of a New Supergroup T

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Aix Marseille Univ, IRD, AP-HM, MEPHI, 13385 Marseille, France
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IHU Méditerranée Infection, 13385 Marseille, France
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Laboratory of Biology and Health, Faculty of Sciences Ben M’sik, Hassan II University, Sidi Othmane, Casablanca 7955, Morocco
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Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France, 13385 Marseille, France
5
Campus Commun UCAD-IRD of Hann, Dakar 10200, Senegal
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(21), 8064; https://doi.org/10.3390/ijms21218064
Received: 5 October 2020 / Revised: 19 October 2020 / Accepted: 23 October 2020 / Published: 29 October 2020
(This article belongs to the Special Issue Advances on Bacterial Genomics)
The symbiotic Wolbachia are the most sophisticated mutualistic bacterium among all insect-associated microbiota. Wolbachia-insect relationship fluctuates from the simple facultative/parasitic to an obligate nutritional-mutualistic association as it was the case of the bedbug-Wolbachia from Cimexlectularius. Understanding this association may help in the control of associated arthropods. Genomic data have proven to be reliable tools in resolving some aspects of these symbiotic associations. Although, Wolbachia appear to be fastidious or uncultivated bacteria which strongly limited their study. Here we proposed Drosophila S2 cell line for the isolation and culture model to study Wolbachia strains. We therefore isolated and characterized a novel Wolbachia strain associated with the bedbug Cimexhemipterus, designated as wChem strain PL13, and proposed Wolbachiamassiliensis sp. nov. strain wChem-PL13 a type strain of this new species from new supergroup T. Phylogenetically, T-supergroup was close to F and S-supergroups from insects and D-supergroup from filarial nematodes. We determined the 1,291,339-bp genome of wChem-PL13, which was the smallest insect-associated Wolbachia genomes. Overall, the wChem genome shared 50% of protein coding genes with the other insect-associated facultative Wolbachia strains. These findings highlight the diversity of Wolbachia genotypes as well as the Wolbachia-host relationship among Cimicinae subfamily. The wChem provides folate and riboflavin vitamins on which the host depends, while the bacteria had a limited translation mechanism suggesting its strong dependence to its hosts. However, the clear-cut distinction between mutualism and parasitism of the wChem in C. hemipterus cannot be yet ruled out. View Full-Text
Keywords: Wolbachia; bedbug; Cimex hemipterus; isolation; culture; genomics; B-vitamins Wolbachia; bedbug; Cimex hemipterus; isolation; culture; genomics; B-vitamins
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MDPI and ACS Style

Laidoudi, Y.; Levasseur, A.; Medkour, H.; Maaloum, M.; Ben Khedher, M.; Sambou, M.; Bassene, H.; Davoust, B.; Fenollar, F.; Raoult, D.; Mediannikov, O. An Earliest Endosymbiont, Wolbachia massiliensis sp. nov., Strain PL13 from the Bed Bug (Cimex hemipterus), Type Strain of a New Supergroup T. Int. J. Mol. Sci. 2020, 21, 8064. https://doi.org/10.3390/ijms21218064

AMA Style

Laidoudi Y, Levasseur A, Medkour H, Maaloum M, Ben Khedher M, Sambou M, Bassene H, Davoust B, Fenollar F, Raoult D, Mediannikov O. An Earliest Endosymbiont, Wolbachia massiliensis sp. nov., Strain PL13 from the Bed Bug (Cimex hemipterus), Type Strain of a New Supergroup T. International Journal of Molecular Sciences. 2020; 21(21):8064. https://doi.org/10.3390/ijms21218064

Chicago/Turabian Style

Laidoudi, Younes, Anthony Levasseur, Hacène Medkour, Mossaab Maaloum, Mariem Ben Khedher, Masse Sambou, Hubert Bassene, Bernard Davoust, Florence Fenollar, Didier Raoult, and Oleg Mediannikov. 2020. "An Earliest Endosymbiont, Wolbachia massiliensis sp. nov., Strain PL13 from the Bed Bug (Cimex hemipterus), Type Strain of a New Supergroup T" International Journal of Molecular Sciences 21, no. 21: 8064. https://doi.org/10.3390/ijms21218064

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