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Production and Biomedical Translation of Functional Biomolecules

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: 30 September 2026 | Viewed by 747

Editors


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Guest Editor
Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy
Interests: mitochondrial bioenergetics; cell metabolism; metabolic engineering; microbial fermentation; biomolecules
Special Issues, Collections and Topics in MDPI journals

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Guest Editor Assistant
Department of Biosciences, Biotechnologies and Environment, University of Bari Aldo Moro, 70125 Bari, Italy
Interests: metabolic engineering; microbial fermentation; biomolecules

Special Issue Information

Dear Colleagues,

Biomolecules—including proteins, peptides, polysaccharides, nucleic acids, lipids, and their engineered derivatives—are fundamental components of living systems and key drivers of biomedical innovation.

The growing integration of recombinant and synthetic production strategies with molecular engineering approaches has opened new perspectives for the development of biomolecules with enhanced stability, specificity, and therapeutic potential. Detailed structural and functional characterization, together with the investigation of biomolecular interactions and mechanistic pathways, is essential to accelerate their biomedical translation and clinical applicability.

This Special Issue aims to provide a comprehensive and integrated overview of current advances in the production, characterization, engineering, and biomedical application of functional biomolecules. We welcome original research articles and reviews addressing recombinant and synthetic production strategies, molecular and structural engineering, functional modification, physicochemical characterization, biomolecular interactions, and mechanistic insights that support translational development.

Topics of interest for publication include, but are not limited to, the following:

  • Recombinant and synthetic production of biomolecules;
  • Structural and physicochemical characterization of biomolecules;
  • Biomolecular interactions and binding mechanisms;
  • Stability, delivery, and formulation of functional biomolecules;
  • Translational applications in diagnostics, therapeutics, and regenerative medicine;
  • Emerging trends in synthetic biotechnology and bioengineering for biomedical innovation.

We look forward to receiving your contributions.

Dr. Loredana Capobianco
Guest Editor

Dr. Giorgia Natalia Iaconisi
Guest Editor Assistant

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-anonymized peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • functional biomolecules
  • recombinant production
  • molecular engineering
  • structural characterization

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Published Papers (1 paper)

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Research

16 pages, 1042 KB  
Article
A Simple Method to Produce Recombinant Mammalian Serum Albumins in Escherichia coli Preserving Intact Antigenic Properties
by Anna S. Dolgova, Anna S. Cherkashina, Alexander I. Shcherbakov, Kseniya A. Lashkevich, Irina A. Goptar, Irina P. Lisyukova, Anna E. Sudina and Olga A. Stukolova
Int. J. Mol. Sci. 2026, 27(11), 4853; https://doi.org/10.3390/ijms27114853 - 28 May 2026
Viewed by 429
Abstract
Serum albumin (SA) plays a fundamental role in the transport of metabolites and endogenous ligands. Additionally, animal albumins are potent allergens. Heterologous expression of SAs is challenging due to their complex structure. In this study, we describe a simple method for enhanced production [...] Read more.
Serum albumin (SA) plays a fundamental role in the transport of metabolites and endogenous ligands. Additionally, animal albumins are potent allergens. Heterologous expression of SAs is challenging due to their complex structure. In this study, we describe a simple method for enhanced production of soluble and functional recombinant feline (rFel d 2) and canine (rCan f 3) albumins in the standard E. coli strain BL21 (DE3). To achieve this, the 18-amino acid signal peptide was removed from the N-terminus of each albumin. To improve expression, folding, and solubility of recombinant proteins, we tested an extended panel of fusion proteins. Among them, MBP (maltose-binding protein), TF (trigger factor), and NusA (E. coli transcription termination factor) allowed the production of soluble fusion forms of rFel d 2 and rCan f 3. To confirm the structural integrity of the products, we analyzed the IgE-binding characteristics of recombinant versus native albumins. rFel d 2 and rCan f 3 fused with TF, MBP, and NusA bound albumin-specific immunoglobulin E (in 26/33, 29/33, 31/33, and 27/29, 26/29, 27/29 cases, respectively, comparable to native Fel d 2 and Can f 3. Thus, removal of the signal peptide combined with fusion partners enables expression of Fel d 2 and Can f 3 in E. coli with preserved antigenic properties. Full article
(This article belongs to the Special Issue Production and Biomedical Translation of Functional Biomolecules)
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