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Glycobiology: The New Discoveries, Approaches, and Technical Developments: 2nd Edition

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Macromolecules".

Deadline for manuscript submissions: 30 November 2025 | Viewed by 961

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Guest Editor
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovacica 1, 10000 Zagreb, Croatia
Interests: galectins; lectin- and glycosyltransferase-gene expression; glycans in nanotechnology; glycobiology of stress; congenital disorders of glycosylation
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Special Issue Information

Dear Colleagues,

Over the last few decades, our understanding of structure and function of carbohydrate has undergone an incredible transformation. From being considered molecules whose role is strictly energetical or structural, glycans are now recognized as complex and diverse structures involved in, and even crucial to, numerous biological processes. Given that the techniques and interest for investigating the structure, presence, and functions of glycans are progressing more and more, it is expected that we will, in the years ahead, discover many more of their roles—some "sweet or desirable", others "bitter or fatal". The growing field of glycobiology has a great impact, not only on our understanding of biological processes, but also on our potential to modulate them. This open access Special Issue will bring together original research and review articles on various aspects of glycobiology, highlighting new discoveries, approaches, and technical developments. The main focus of this issue is sharing significant works that advance our understanding of glycans’ roles and their impact on the structure and function of individual molecules, materials, cells, tissues, and organisms, as well as on the interplay between them.

This Special Issue is supervised by Dr. Sanja Dabelić and assisted by our Guest Editor Assistant Dr. Branimir Plavša (Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia).  We welcome original research papers, as well as critical or comprehensive reviews, that demonstrate or summarize significant advances in the glycobiology field. 

Topics include, but are not limited to:

  • Novel tools and strategies for the analysis/synthesis/modulation of glycoconjugates.
  • Glycan structure, function, and biosynthesis.
  • Glycan-recognizing molecules.
  • Glycan structural and functional diversity across biological taxa.
  • Genetic basis of glycobiology.
  • Glycans in physiology, disorders, and diseases.
  • Carbohydrates in pharmacy, medicine, biotechnology, materials sciences and nanotechnology.

Dr. Sanja Dabelić
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • glycans
  • lectins
  • glycoconjugates
  • glycosylation
  • genetic disorders of glycosylation
  • glycomics and glycoproteomics

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Published Papers (2 papers)

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Research

13 pages, 1934 KB  
Article
Chemical Structure of a Branched α-d-Glucan from the Eggs of Sea Urchin Tripneustes gratilla
by Maria I. Bilan, Dmitry A. Argunov, Vladimir I. Torgov, Andrey S. Dmitrenok, Dinh Thanh Trung, Thinh Duc Pham, Hang Thi Thuy Cao, Anatolii I. Usov and Nikolay E. Nifantiev
Int. J. Mol. Sci. 2025, 26(21), 10326; https://doi.org/10.3390/ijms262110326 - 23 Oct 2025
Abstract
A water-soluble high-molecular neutral polysaccharide (NP) was isolated from the eggs of the sea urchin Tripneustes gratilla. The formation of glucose only upon the treatment of NP by amyloglucosidase and the value of its optical rotation [α]D +233.5 (c [...] Read more.
A water-soluble high-molecular neutral polysaccharide (NP) was isolated from the eggs of the sea urchin Tripneustes gratilla. The formation of glucose only upon the treatment of NP by amyloglucosidase and the value of its optical rotation [α]D +233.5 (c 0.2, water) confirmed its belonging to the family of α-d-glucans. According to the results of NMR spectroscopy and methylation analysis, the chains of NP are built up of non-reducing terminal, 4-linked and 4,6-disubstituted glucose residues at a ratio of 1:8:1. A branched structure with an average linear chain length of about five glucose residues was calculated from the spectrum of iodine complex. Contrary to the previously published structure of branched α-d-glucan from the sea urchin Strongylocentrotus nudus bearing single glucose units as branches, the polysaccharide NP contains oligosaccharide branches at position 6, which was confirmed by NMR data. Hence, NP has a glycogen-like structure with a rather high degree of branching, which markedly exceeds that of usual mammalian or fungal glycogens. Full article
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17 pages, 1246 KB  
Article
Simultaneous Determination of Reducing Sugars in Honey by Capillary Zone Electrophoresis with LIF Detection Using Low-Toxicity 2-Picoline Borane and APTS for Pre-Capillary Derivatization
by Joanna Bulesowska, Michał Pieckowski, Piotr Kowalski, Tomasz Bączek and Ilona Olędzka
Int. J. Mol. Sci. 2025, 26(15), 7569; https://doi.org/10.3390/ijms26157569 - 5 Aug 2025
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Abstract
This study aimed to develop a reliable method for profiling reducing sugars in honey using capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF). Reducing sugars were derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) in the presence of 2-picoline borane, a safer alternative to sodium cyanoborohydride. [...] Read more.
This study aimed to develop a reliable method for profiling reducing sugars in honey using capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF). Reducing sugars were derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) in the presence of 2-picoline borane, a safer alternative to sodium cyanoborohydride. Key parameters influencing the derivatization efficiency—temperature, pH, incubation time, and reagent concentrations—were systematically optimized. The highest labeling efficiency for glucose, mannose, and maltose was achieved at 50 °C in 0.5 M citric acid with 0.1 M APTS, while fructose showed low reactivity due to its ketose structure. To reduce the background signal from excess reagents, three cleanup strategies were evaluated. Liquid–liquid extraction with ethyl acetate effectively removed unreacted APTS without significant analyte loss, whereas solid-phase extraction and microextraction caused substantial losses of hydrophilic sugars. The method showed good linearity (0.5–10 mM, R2 > 0.994), precision (RSD 0.81–13.73%), and accuracy (recoveries 93.47–119.75%). Stability studies indicated that sugar standards should be stored at –20 °C. The method was successfully applied to the analysis of four nectar honeys—rapeseed, acacia, phacelia, and dandelion—revealing differences in glucose and fructose content related to botanical origin. The results confirm the suitability of CZE-LIF for sensitive and selective carbohydrate analyses in complex food matrices. Full article
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