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Advances in Microbial Strategies to Combat Antimicrobial Resistance

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 30 September 2026 | Viewed by 247

Editor


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Guest Editor
Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand
Interests: Probiotics; Postbiotics; Antimicrobial resistance; Bacteriophages; Microbial bioactives; Functional foods; Molecular microbiology; Biomedical innovation

Special Issue Information

Dear Colleagues,

Antimicrobial resistance (AMR) has become a major global health concern, posing significant challenges to the effective treatment of infectious diseases. The rapid emergence of multidrug-resistant pathogens necessitates the development of innovative and sustainable strategies to combat microbial infections.

This Special Issue aims to present recent advances in microbiology, molecular biology, and biomedical sciences that contribute to addressing AMR. We welcome original research articles and comprehensive reviews focusing on probiotics, postbiotics, bacteriophages, and microbial-derived bioactive compounds with antimicrobial, antibiofilm, antioxidant, and immunomodulatory activities.

Particular emphasis will be placed on studies exploring the molecular mechanisms underlying antimicrobial resistance, host–microbe interactions, and novel therapeutic approaches, including functional foods and diagnostic technologies. Contributions utilizing omics technologies, molecular diagnostics, and translational research approaches are especially encouraged to bridge the gap between laboratory discoveries and clinical applications.

Research targeting clinically relevant multidrug-resistant pathogens, including ESKAPE organisms, will be of high interest. By integrating multidisciplinary perspectives, this Special Issue aims to provide a comprehensive platform for advancing knowledge and fostering innovative solutions to mitigate AMR challenges in both healthcare and community settings.

Dr. Chonticha Romyasamit
Guest Editor

Manuscript Submission Information

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Keywords

  • probiotics
  • postbiotics
  • antimicrobial resistance
  • bacteriophages
  • microbial bioactives
  • functional foods
  • molecular microbiology
  • biomedical innovation

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Published Papers (1 paper)

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Research

17 pages, 1900 KB  
Article
Comparative Genomic and Functional Characterization of Pediococcus acidilactici Isolated from Fermented Cacao with Anti-ESKAPE Activity
by Pinkanok Suksabay, Yosita Leepromma, Benyapa Prakit, Tansuda Puchong, Joo Shun Tan and Chonticha Romyasamit
Int. J. Mol. Sci. 2026, 27(13), 5996; https://doi.org/10.3390/ijms27135996 - 3 Jul 2026
Abstract
ESKAPE pathogens have become a major global health challenge. This study aimed to isolate and characterize LAB from fermented cacao and to evaluate their probiotic properties, preliminary antimicrobial activity against ESKAPE pathogens, safety profiles, and functional bioactivities. Each of P. acidilactici isolates exhibited [...] Read more.
ESKAPE pathogens have become a major global health challenge. This study aimed to isolate and characterize LAB from fermented cacao and to evaluate their probiotic properties, preliminary antimicrobial activity against ESKAPE pathogens, safety profiles, and functional bioactivities. Each of P. acidilactici isolates exhibited antibacterial activity against ESKAPE pathogens, with inhibition zone diameters ranging from 10.00 ± 1.00 mm to 23.00 ± 0.00 mm, depending on the isolate and pathogens tested. CR05 was identified as the most promising probiotic candidate, showing the highest survival at pH 2 (69.65 ± 6.66%), strong tolerance to pancreatin (99.95 ± 0.10%), pepsin (89.11 ± 2.38%), bile salts (98.65 ± 0.33%), and favorable adhesion properties, including auto-aggregation, cell surface hydrophobicity, and adhesion to HT-29 intestinal epithelial cells. The safety assessment indicated a notable susceptibility to gentamicin, tetracycline, and chloramphenicol, with resistance to several other tested antibiotics. and showed no hemolytic activity. Three selected isolates were evaluated for minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against ESKAPE pathogens, with MIC and MBC values ranging from 12.5 to 25 mg/mL and 25 to >50 mg/mL, respectively. The strongest inhibitory activity was observed against A. baumannii, P. aeruginosa, and E. aerogenes, with MIC values of 12.5 mg/mL, particularly for isolates CR05 and CR06. Whole-genome analysis identified genes related to stress response and gastrointestinal tolerance and predicted the enterolysin A gene. No acquired antimicrobial resistance genes were detected. These findings suggest that P. acidilactici isolates from fermented cacao are promising probiotic candidates for further investigation in functional food, probiotic, and postbiotic-related applications. Full article
(This article belongs to the Special Issue Advances in Microbial Strategies to Combat Antimicrobial Resistance)
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