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The Blood–Brain Barrier and Neuroprotection

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Neurobiology".

Deadline for manuscript submissions: 20 September 2025 | Viewed by 886

Special Issue Editors


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Guest Editor
Department of Surgical Sciences, Akademiska Sjukhuset, Uppsala University, Uppsala, Sweden
Interests: neurotoxicity; anesthesia; brain function; blood-brain barrier; morphology

E-Mail Website
Guest Editor
Division of Anesthesiology & Intensive Care Medicine, Department of Surgical Sciences, Uppsala University Hospital, Uppsala University, Uppsala, Sweden
Interests: neurobiology; blood brain barrier (BBB); brain dysfunction

Special Issue Information

Dear Colleagues,

The blood–brain barrier (BBB) plays a regulatory role of the central nervous  system (CNS) in maintaining homeostasis for normal brain function. In acute  or chronic diseases, the BBB breakdown occurs, leading to extravasation of  unwanted substances, e.g., proteins, hormones, toxins, and other harmful  elements, into the brain, resulting in brain pathology. Thus, regulation of  the BBB is one of the critical factors in health and disease. This Special Issue aims to explore the role of the BBB in modifying the disease processes using conventional and nanoformulations to maintain the integrity of the BBB in neurodegenerative diseases in pre-clinical and clinical conditions. Involvement of the molecular mechanisms of BBB integrity and repair will be discussed in various acute and chronic neurodegenerative diseases. We hope that this Special Issue will widen our understanding of BBB and brain diseases and enhance our knowledge of therapy.

Dr. Aruna Sharma
Prof. Dr. Hari Shanker Sharma
Guest Editors

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Keywords

  • blood–brain barrier
  • neurodegenerative diseases
  • nanoformulations
  • drug development
  • delivery systems

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Published Papers (1 paper)

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Research

20 pages, 15637 KiB  
Article
Ex Vivo Plasma Application on Human Brain Microvascular Endothelial-like Cells for Blood–Brain Barrier Modeling
by Sophie-Charlotte Nelz, Elisabeth Lück, Anne Schölzel, Martin Sauer, Jacqueline Heskamp and Sandra Doss
Int. J. Mol. Sci. 2025, 26(7), 3334; https://doi.org/10.3390/ijms26073334 - 3 Apr 2025
Viewed by 491
Abstract
hiPSC-derived blood–brain barrier (BBB) models are valuable for pharmacological and physiological studies, yet their translational potential is limited due to insufficient cell phenotypes and the neglection of the complex environment of the BBB. This study evaluates the plasma compatibility with hiPSC-derived microvascular endothelial-like [...] Read more.
hiPSC-derived blood–brain barrier (BBB) models are valuable for pharmacological and physiological studies, yet their translational potential is limited due to insufficient cell phenotypes and the neglection of the complex environment of the BBB. This study evaluates the plasma compatibility with hiPSC-derived microvascular endothelial-like cells to enhance the translational potential of in vitro BBB models. Therefore, plasma samples (sodium/lithium heparin, citrate, EDTA) and serum from healthy donors were tested on hiPSC-derived microvascular endothelial-like cells at concentrations of 100%, 75%, and 50%. After 24 h, cell viability parameters were assessed. The impact of heparin-anticoagulated plasmas was further evaluated regarding barrier function and endothelial phenotype of differentiated endothelial-like cells. Finally, sodium-heparin plasma was tested in an isogenic triple-culture BBB model with continuous TEER measurements for 72 h. Only the application of heparin-anticoagulated plasmas did not significantly alter viability parameters compared to medium. Furthermore, heparin plasmas improved barrier function without increasing cell density and induced a von Willebrand factor signal. Finally, continuous TEER measurements of the triple-culture model confirmed the positive impact of sodium-heparin plasma on barrier function. Consequently, heparin-anticoagulated plasmas were proven to be compatible with hiPSC-derived microvascular endothelial-like cells. Thereby, the translational potential of BBB models can be substantially improved in the future. Full article
(This article belongs to the Special Issue The Blood–Brain Barrier and Neuroprotection)
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