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Advances in Brassica Crop Metabolism and Genetics (Second Edition)

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: 30 October 2025 | Viewed by 485

Special Issue Editors


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Guest Editor
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Interests: vegetable crops; molecular genetic breeding; germplasm innovation; genome design; gene editing; multi-omics techniques
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Special Issue Information

Dear Colleagues,

Brassicas are among the most economically valuable corps in the Cruciferae family, containing multiple important vegetables, oils and ornamental crops. Brassica includes three basic diploid species, namely Chinese cabbage (Brassica rapa, AA, 2n = 20), black mustard (B. nigra, BB, 2n = 16) and cabbage (B. oleracea, CC, 2n = 18), as well as three tetraploid hybrid species: oilseed rapes (B. napus, AACC, 2n=38), mustard (B. juncea, AABB, 2n = 36), and Ethiopian mustard (B. carinata, BBCC, 2n=34). Several subspecies, varieties and ecotypes of Brassica have been derived via interspecific hybridization within the genus, which greatly enriches the genetic resources of Brassica and provides an excellent model for the study of polyploid speciation, evolution and selection.

The Brassica genus boasts immense economic, ecological and nutritional value. As staple crops, Brassica species contribute significantly to food security. Beyond their culinary uses, Brassicas offer various industrial applications, such as bioactive compound extraction from broccoli or mustard seeds. Moreover, these plants play a crucial role in sustainable agriculture, with some species exhibiting natural pest resistance and soil improvement properties. In recent years, genome sequencing and various omics technologies have gradually clarified the metabolic pathways and regulatory networks of the secondary metabolites of Brassica, providing a way to improve the nutritional quality and flavor or the resistance of brassica crops to environmental stress through metabolic engineering methods.

For this Special Issue, we welcome any original research articles, reviews, short notes or opinion articles that highlight the metabolic regulation and genetic improvement of Brassica crops, such as genomics, transcriptomics, proteomics, metabolomics, signal regulation network and genome editing. This Special Issue welcomes contributions that explore various aspects of the Brassica genus, including but not limited to taxonomy, genetics, breeding, agronomy, phytochemistry and biotechnology. Manuscripts elucidating the phylogeny and taxonomy of Brassica crops, metabolite synthesis and regulation networks, as well as the physiological traits (nutritional quality, special flavor) and biotic/abiotic stress responses of Brassica species, are particularly encouraged.

Prof. Dr. Bo Sun
Dr. Honghao Lv
Guest Editors

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Keywords

  • brassica crop
  • genomics
  • transcriptomics
  • proteomics
  • metabolomics
  • gene editing
  • metabolite synthesis and regulation
  • phylogeny and taxonomy
  • physiological traits
  • biotic/abiotic stresses
  • biotechnology

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Published Papers (1 paper)

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Research

32 pages, 5733 KiB  
Article
Metabolomic Profiling Identifies Key Metabolites and Defense Pathways in Rlm1-Mediated Blackleg Resistance in Canola
by Xiaohan Zhu, Peng Gao, Shuang Zhao, Xian Luo, Liang Li and Gary Peng
Int. J. Mol. Sci. 2025, 26(12), 5627; https://doi.org/10.3390/ijms26125627 - 12 Jun 2025
Viewed by 345
Abstract
Blackleg disease poses a major threat to global canola production. The resistance gene Rlm1, corresponding to the avirulence gene AvrLm1 in the pathogen Leptosphaeria maculans, has been widely used to mitigate the impact of the disease. To investigate the biochemical basis of [...] Read more.
Blackleg disease poses a major threat to global canola production. The resistance gene Rlm1, corresponding to the avirulence gene AvrLm1 in the pathogen Leptosphaeria maculans, has been widely used to mitigate the impact of the disease. To investigate the biochemical basis of Rlm1-mediated resistance against blackleg, we conducted an LC-MS–based analysis of a susceptible Topas double haploid (DH) line and its isogenic Rlm1-carrying resistant counterpart for metabolomic profiles during the infection process. Samples were labeled with 12C- and 13C for LC-MS analyses to enhance both chemical and physical properties of metabolites for improved quantification and detection sensitivity. Resistant plants showed early and sustained accumulation of several defense metabolites, notably pipecolic acid (PA, up to 326-fold), salicylic acid (SA), and gentisic acid (GA) in L. maculans-inoculated Topas–Rlm1 plants compared to mock-inoculated Topas–Rlm1 controls (adjusted p < 0.05), indicating activation of lysine degradation and hormonal defense pathways. Elevated glucosinolates (GLS), γ-aminobutyric acid (GABA), and melatonin precursors may further contribute to antimicrobial defense and cell-wall reinforcement. In contrast, flavonoid and phenylpropanoid pathways were down-regulated, suggesting metabolic reallocation during resistance. Exogenous application of PA, SA, GA, ferulic acid, and piperonylic acid (a known inhibitor of the phenylpropanoid pathway in plants) significantly reduced infection in susceptible canola varieties, validating their defense roles against blackleg. These results offer new insights into Rlm1-mediated resistance and support metabolic targets for breeding durable blackleg resistance in canola. Full article
(This article belongs to the Special Issue Advances in Brassica Crop Metabolism and Genetics (Second Edition))
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