Next Article in Journal
Multiomics Analysis Reveals Role of ncRNA in Hypoxia of Mouse Brain Microvascular Endothelial Cells
Previous Article in Journal
Inhibition of Soluble Epoxide Hydrolase Prevents Docetaxel-Induced Painful Peripheral Neuropathy
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Metabolomic Profiling Identifies Key Metabolites and Defense Pathways in Rlm1-Mediated Blackleg Resistance in Canola

1
Saskatoon Research and Development Center, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK S7N 0X2, Canada
2
The Metabolomics Innovation Centre and Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2025, 26(12), 5627; https://doi.org/10.3390/ijms26125627
Submission received: 3 April 2025 / Revised: 3 June 2025 / Accepted: 6 June 2025 / Published: 12 June 2025
(This article belongs to the Special Issue Advances in Brassica Crop Metabolism and Genetics (Second Edition))

Abstract

Blackleg disease poses a major threat to global canola production. The resistance gene Rlm1, corresponding to the avirulence gene AvrLm1 in the pathogen Leptosphaeria maculans, has been widely used to mitigate the impact of the disease. To investigate the biochemical basis of Rlm1-mediated resistance against blackleg, we conducted an LC-MS–based analysis of a susceptible Topas double haploid (DH) line and its isogenic Rlm1-carrying resistant counterpart for metabolomic profiles during the infection process. Samples were labeled with 12C- and 13C for LC-MS analyses to enhance both chemical and physical properties of metabolites for improved quantification and detection sensitivity. Resistant plants showed early and sustained accumulation of several defense metabolites, notably pipecolic acid (PA, up to 326-fold), salicylic acid (SA), and gentisic acid (GA) in L. maculans-inoculated Topas–Rlm1 plants compared to mock-inoculated Topas–Rlm1 controls (adjusted p < 0.05), indicating activation of lysine degradation and hormonal defense pathways. Elevated glucosinolates (GLS), γ-aminobutyric acid (GABA), and melatonin precursors may further contribute to antimicrobial defense and cell-wall reinforcement. In contrast, flavonoid and phenylpropanoid pathways were down-regulated, suggesting metabolic reallocation during resistance. Exogenous application of PA, SA, GA, ferulic acid, and piperonylic acid (a known inhibitor of the phenylpropanoid pathway in plants) significantly reduced infection in susceptible canola varieties, validating their defense roles against blackleg. These results offer new insights into Rlm1-mediated resistance and support metabolic targets for breeding durable blackleg resistance in canola.

Graphical Abstract

1. Introduction

Canola/rapeseed (Brassica napus L.) is a major cash crop in Canada, with national production reaching 17.8 million tons in 2024 and contributing nearly CAD 40 billion to the Canadian economy [1]. Blackleg, caused by L. maculans Ces. and de Not., poses a serious threat to canola production in Canada, with estimated global economic losses exceeding USD 900 million annually [2,3]. Effective management of blackleg is therefore critical for a successful canola crop.
Genetic resistance, in combination with extended crop rotation, is the key strategy for blackleg management in Canada, where many canola cultivars carry the specific resistance (R) genes Rlm1/LepR3 and Rlm3 [4], while almost all cultivars also possess a level of quantitative resistance [5,6]. The R gene Rlm1 has been deployed in B. napus cultivars since the mid 1990s [7]. As more R genes are being deployed in new cultivars that target prevalent avirulence (Avr) genes in the pathogen population [8,9,10], resistance resilience ought to be monitored because of frequent sexual recombination of L. maculans and the high evolutionary potential of the pathogen [11,12]. For instance, Rlm1 was broken only a few years after its release in France, with a significant decline in the corresponding AvrLm1 from 83% to <13% in the pathogen population [12]. In western Canada, Rlm1 is no longer effective since 2007 due to the low presence of the corresponding avirulence gene AvrLm1 in the pathogen population [8,9]. To date, 22 specific R genes have been reported for blackleg resistance, of which 5 have been successfully cloned (reviewed by Borhan, Van de Wouw [13]). The effective deployment of R genes depends mostly on the Avr profile of the L. maculans population, but a better understanding of the resistance mode of action associated with specific R genes can improve our knowledge of host–pathogen interactions in relation to resistance deployment and resilience.
Advances in next-generation sequencing (NGS) and other “omics” technologies have helped identify preliminary mechanisms underlying disease resistance in canola [14,15]. However, such information has not been available for most of the blackleg R genes, especially for key metabolites and defense pathways involved in ‘gene-for-gene’ interactions. In an earlier report, Fudal, Ross [16] described that L. maculans appeared to delete AvrLm1 to regain the virulence toward Rlm1 worldwide. In a separate study, transcriptome analysis revealed that Rlm1-mediated resistance functions locally rather than systemically and involves activation of both salicylic acid (SA) and jasmonic acid (JA) signaling pathways [17]. These findings offer foundational insights into the mechanisms of Rlm1 resistance and its potential vulnerability to shifts in pathogen populations. Building on this, metabolomic analysis may uncover key metabolites and defense-related pathways directly linked to Rlm1 function, providing a deeper understanding of its role in blackleg infection and disease progression.
During co-evolution, many plants have developed resistance to diseases, which can often be triggered by pathogen-associated molecular patterns and effectors [18,19,20]. As part of their defense responses, plants can produce a range of low-molecular-weight compounds [21], many of which are secondary metabolites belonging broadly to flavonoids (Flav) and phenolics, alkaloids and sulfur-containing compounds, and terpenoids [22,23]. Many of them play a role in plant defense against pathogens [24,25]. Metabolomic study of canola–L. maculans interactions can deepen our knowledge of blackleg resistance, uncover novel plant defense compounds and pathways, and support disease resistance breeding efforts by targeting key metabolites or their associated genes.
Metabolomic studies commonly employ high-performance liquid chromatography (HPLC), ultra-performance liquid chromatography (UPLC), gas chromatography (GC), and nuclear magnetic resonance (NMR), often coupled with mass spectrometry (MS) [26]. Among these, LC–MS is widely used due to its high sensitivity in detecting metabolites [27]. However, LC–MS can have limitations in terms of metabolite coverage and quantitative accuracy [27]. To address these challenges, chemical isotope labeling (CIL) techniques—such as differential 12C-/13C-isotope dansylation—have been used to help improve the quantification and sensitivity of metabolomic analysis [28]. When combined with MS, CIL enables broader metabolite coverage across various species and tissue types [27,29,30,31].
Several key metabolites identified through metabolomics have been implicated in plant defense responses. For example, amino acids such as lysine (Lys) play a regulatory role in the activity of defense-related proteins, including β-1,3-glucanase, chitinase, and enzymes involved in reactive oxygen species (ROS) regulation [32]. Lys also contributes to systemic acquired resistance (SAR) by serving as a precursor to pipecolic acid (PA) [33], a known SAR signaling molecule that functions alongside salicylic acid (SA) [34] and redox-related mechanisms [35]. Additionally, the accumulation of γ-aminobutyric acid (GABA) has been associated with enhanced disease resistance in rice, Arabidopsis, and other plant species [36,37,38]. Methionine (Met) cycle enzymes, along with ethylene (ET) and polyamines, are also involved in resistance to viral pathogens [39]. Furthermore, gentisic acid (GA) has been shown to activate peroxidase activity in cucumber and purple velvet (Gynura aurantiaca) following pathogen infection or SA treatment [40].
Here, we report a study investigating the metabolomic basis of Rlm1-mediated blackleg resistance in canola using the CIL LC–MS platform. By identifying key metabolites and associated post-transcriptional defense-related pathways, this research seeks to enhance our understanding of the molecular mechanisms underlying resistance. Building on our previous transcriptome analysis of Rlm1-mediated responses [17], our specific objectives were as follows: (i) characterize metabolomic changes during the infection process; (ii) identify metabolic pathways that contribute to host defense; and (iii) validate the functional roles of highly accumulated metabolites involved in Rlm1-mediated resistance to blackleg disease in canola.

2. Results

2.1. Multivariate Analysis of Metabolomic Data

Multivariate analysis using PCA was performed to identify distinct metabolomic clustering patterns between resistant and susceptible lines following infection, reflecting treatment- and time-specific metabolic responses. More than 3000 metabolites were identified in susceptible (Topas) and resistant (Topas–Rlm1) canola varieties with or without L. maculans (AvrLm1) inoculation. For clarity, these treatments were labelled as TC (Topas-control), TI (Topas-inoculated), RC (Rlm1-control), and RI (Rlm1-inoculated), corresponding to 3, 7, or 11 days post-inoculation (dpi). The first two principal components of PCA (PC1: 28.2%, PC2: 13.9%) explained the variance (Figure S1). Samples from each treatment and time point formed distinct clusters, demonstrating the data reproducibility. Additionally, QC samples clustered together, indicating high experimental consistency.
At 11 dpi, the TI and RI groups were distinct from other groups and were also separated from each other along PC1 (Figure S1). Additionally, the RC-3dpi, RI-3dpi, and RI-7dpi groups were also distinct from the rest. Notably, all RI samples were separated from those of TI at each sampling point. Relatively, PC1 accounted for most of the variance across time points, while PC2 captured the majority of the variance among treatments.

2.2. Univariate Analysis of Metabolomic Data

Univariate statistical analysis was conducted to identify metabolites that were differentially accumulated or suppressed across time points and treatments. Between the control groups TC and RC, a total of 591, 586, and 452 metabolites exhibited increases, while 436, 365, and 385 showed decreases at 3, 7, and 11 dpi (Figure S2). However, between inoculated RI and its water control RC, 259, 457, and 687 metabolites increased, while 469, 392, and 692 decreased at the respective time points. For TI and TC, the corresponding numbers were 169, 317, and 934 (increase) and 181, 198, and 644 (decrease).
Compared to TI, the resistant RI responded more rapidly to infection, with 259 and 457 up-regulated DAMs at 3 and 7 dpi (Figure S2). In contrast, TI displayed only 169 and 317 up-regulated DAMs relative to its control TC at these two time points. In the meantime, RI showed 469 and 392 down-regulated DAMs, while TI had only 181 and 198, relative to their respective controls at 3 and 7 dpi. At 11 dpi, however, TI exhibited the highest number of up-regulated DAMs (934) among all treatments.

2.3. DAMs in Relation to Inoculation and Resistance

Venn diagram analysis was used to compare up- and down-regulated DAMs across time points and treatments in resistant and susceptible canola lines, with or without L. maculans inoculation, at various infection stages. A significant number of DAMs were shared between RI and its control RC across the time points, indicating the involvement of many of the same metabolites in the Topas–Rlm1, with or without infection across 3–11 dpi (Figure 1A–D). This pattern, however, was much less pronounced between TI and TC.
Further comparisons among samples collected at the same dpi showed that only a limited number of DAMs were shared between RI and TI at 3 and 7 dpi (Figure 2A–D). However, at 11 dpi, the number of overlapping up- and down-regulated DAMs increased sharply between the two treatments, reaching 450 and 277, respectively (Figure 2E,F), indicating that many DAMs present in RI were also found in TI at the later stage of infection.

2.4. Prominent DAMs and Their Related Pathways

To identify pathways associated with different infection stages in the Topas–Rlm1, significantly regulated DAMs at each stage were subjected to pathway analysis. Following the initial analyses above, the most significantly regulated DAMs associated with resistance were identified and further analyzed for potential pathways involved. Out of 1145 up- and 1466 down-accumulated relevant peaks, only 299 and 237 had a single confident match to a known compound in the CIL standard (Tier-1) [41], LIL (Tier-2) [42], and MCIDL (Tier-3) [43]. The remaining peaks either lacked matches or had multiple ambiguous matches and were therefore classified as unknown compounds.
Many of the DAMs exhibited earlier responses in RI samples compared to TI, as shown by the heatmaps of Hierarchical Cluster Analysis (Figure 3). Several DAMs displayed significant regulation only between RI and TI. Out of the significant peaks from 3-dpi samples, only 109 matched known metabolites, with 24 being identified by the CIL standard, 47 by LIL, and 38 by MCIDL. To ensure reliability, only DAMs identified using Tier-1 and Tier-2 libraries were analyzed for pathways in the Arabidopsis database [44].
At 3 dpi, several DAMs were significantly regulated in the Rlm1-mediated resistance (RI) compared to RC (mock). These metabolites are associated with the biosynthesis of Lys (meso-2,6-diaminoheptanedioic acid) and anthocyanin-ACN (pelargonidin 3-O-β-D-sambubioside), degradation of Lys (PA), and metabolism of purine (adenosine), pyrimidine (cytidine), arginine, and proline [N-carbamoylputrescine (NCP)] (Table 1). In contrast, several DAMs were significantly down-regulated in the RI, including those involved in the biosynthesis of flavones and flavonols [kaempferol 3-O-glucoside, quercitrin, kaempferol, quercetin 3-O-rhamnoside 7-O-glucoside (QRG)], Flav [kaempferol, (-)-epicatechin (EC)], ubiquinone and other terpenoid-quinones [tyrosine (Tyr)], isoquinoline alkaloids (Tyr), and indole alkaloids [tryptophan (Trp)] (Table 1). However, several DAMs identified in RI at 3 dpi were not enriched during pathway analysis, including salicylate β-D-glucose ester (bound SA), (-)-medicarpin, pyrazolidine (PZD), E-6′-HF, γ-glutamyl-β-amino-propiononitrile (γ-Glu-β-APN), m-coumaric acid, and trans-2,3-dihydroxy-cinnamate (t-2,3-DHC).
At 7 dpi, 178 of the 849 distinguished peaks matched known metabolites, and the most significantly regulated DAMs in RI are involved in the metabolism of glutathione (GSH, GSSG, γ-glutamylcysteine), Trp [5-hydroxykynurenamine (5-HKA), serotonin], arginine and proline [γ-aminobutyric acid (GABA), 4-hydroxyproline], pyrimidine (cytidine), and cysteine and methionine-Met [cystathionine (Cth), Met], as well as the biosynthesis of tropane (Trop), piperidine (Pid) and pyridine alkaloid (5-aminopentanal). Some are also involved in the biosynthesis of Lys (Diaminopimelic acid -DAP), Tyr, glucosinolates (GSLs), and ACN, as well as the degradation of Lys [aminoadipic acid (AAA), PA, saccharopine (SAP)] (Table 2). Other DAMs showed decreases in RI, especially those involved in the biosynthesis of flavones and flavonols (quercitrin, luteolin), phenylpropanoids-PPs (ferulate), and arginine (Arg), as well as in the metabolism of glycine (Gly), serine (Ser), and threonine (Thr). Additionally, E-6′-HF, salicylic acid (SA), bound SA, GA, Nε,Nε-dimethyllysine, 5-aminopentanoic acid (5-APA), 3-hydroxymandelic acid (3-HMA), phloroglucinol (PG), dityrosine (DiY), 2,5-dihydroxypyridine (2,5-DHP), emodin (EMD), malonylgenistin (MG), 3,6,7,4′-tetramethylquercetagetin (TMQ), aminoacetaldehyde (AALD), and γ-L-glutamylputrescine (GGP) also increased, while 5-aminolevulinic acid, prolyl-Gly, 3,4-dihydroxybenzaldehyde (3,4-DHBA), and sodium dehydroacetic acid decreased in the RI-7dpi.
At 11 dpi, 287 out of 1379 distinguished peaks from the RI matched known metabolites and the most up-regulated DAMs involved in the biosynthesis of Trop, Pid, and 5-aminopentanal; Lys (DAP), GSLs [homomethionine (homo-Met), p-hydroxyphenylacetothiohydroximate (p-HPAH), phenylalanine (Phe)], ACN (cyanidin 3-O-β-D-sambubioside), Phe, Tyr, and Trp [Phe, 3-(4-Hydroxyphenyl)pyruvate (3-HPP), 2-aminobenzoic acid (2-AA)] also showed significant accumulation (Table 3). Also, DAMs associated with the metabolism of Trp [N-acetylserotonin (NAS), 5-HKA, serotonin], Tyr [3,4-dihydroxymandelaldehyde, homogentisate (HGA), 3-HPP], Phe (Phe) and purines (adenosine monophosphate, hypoxanthine), and Arg and proline (GABA, NCP), as well as Lys degradation (AAA, PA, SAP), were up-regulated relative to the RC control (Table 3). In contrast, DAMs related to biosynthesis of PPs [5-O-caffeoylshikimic acid (5-O-CFSA), ferulate, caffeate], flavones/flavonols (luteolin, QRG), and Flav (p-coumaroyl quinic acid (p-CQA), EC) were down-regulated in RI.
Several DAMs not enriched in the Arabidopsis database but significantly up-regulated in RI included GA, bound SA, SA, PG, methylcysteine, 2-(methylamino)BA, DiY, PZD, AALD, 3-formylsalicylic acid, 3-dechloroethylifosfamide, mangiferin, GGP, MG, E-6′-HF, 2,5-DHP, S-ribosyl-L-homocysteine, TMQ, and dimethylamine. Conversely, kaempferol 3-O-(6″-O-p-coumaroyl)-glucoside, trans-2,3-dihydroxycinnamate (trans-2,3-DHC), bisdemethoxycurcumin (BDMC), and chlorogenic acid (CGA) were the most significantly down-regulated.

2.5. Metabolites/Pathways Potentially Related to Rlm1-Mediated Resistance

To clarify the metabolic responses underlying Rlm1-mediated resistance, key functional groups were identified based on pathway enrichment and patterns of metabolite accumulation using the Arabidopsis database [44] or previously reported data [42,43], covering all time points. In RI, biosynthesis pathways for Lys, GSL, GABA, bound SA, SA, GA, melatonin (Mel), ACN, scopoletin (SCF)/isoscopoletin (IsoScp), and metabolism pathway of NaN, Trp, Tau/HTau, Phe, and amino acids were most activated between 3 and 11 dpi, compared to non-inoculated resistant (RC) or inoculated susceptible (TI) (Figure 4).

2.5.1. Lysine Metabolism and Degradation

Between 3 and 11 dpi, twelve metabolites associated with Lys biosynthesis or degradation were significantly up-regulated in RI (Figure 4A,B). These included PA, SAP, AAA, Nε,Nε-dimethyllysine, N6-acetyl-lysine, 5-APA, 5-hydroxylysine, DAP, and meso-2,6-DAP. Notably, PA exhibited a dramatic increase in RI compared to RC, with fold changes ranging from 164 to 326 across the time points, and was also higher in RI than in TI (Figure 4A). Additionally, peak 1861, tentatively identified as D-1-piperideine-2-carboxylic acid and (S)-2,3,4,5-tetrahydro-piperidine-2-carboxylate (based on Tier-3 library matches), also increased substantially by 11 dpi (Figure 4A). Both compounds are likely intermediates in Lys degradation pathways [61,62].

2.5.2. Defense Signaling Molecules

Metabolites involved in defense signaling, including GA, GABA, SA, and bound SA, were among the most highly accumulated compounds in RI during the infection period (3–11 dpi), with GA reaching peak levels at 11 dpi (Figure 4H,J). The accumulation of benzoic acid (BA) was comparable in RI and TI, but both had lower BA levels than RC, which exhibited the highest levels among all treatments (Figure 4J & Supplementary Figure S3F).

2.5.3. Antimicrobial Metabolites

Several DAMs known for antimicrobial activity were strongly up-regulated in RI (Figure 4K). These included PG, E-6′-HF, MG, 2-aminophenol (2-AP), 2-AA, EMD, PZD, 5-HKA, NCP, and γ-Glu-β-APN. E-6′-HF exhibited a striking 27- to 580-fold increase in RI relative to RC between 3 and 11 dpi (Figure 4E,K). PG, 2-AA, 2-AP, γ-Glu-β-APN, mangiferin, and (-)-medicarpin also showed significant increases in RI, but not in TI, when compared to their respective controls (Figure 4K).

2.5.4. Amino Acid and Secondary Metabolite Pathways

Several DAMs associated with amino acid and secondary metabolite pathways were elevated in RI (Figure 4G,N,O,Q). These included metabolites involved in Trp, NaN, Tau/HTau, and Phe metabolism. In Trp metabolism, NAS, 5-HKA, and 5-hydroxyindoleacetylglycine were significantly increased in RI (Figure 4N). Peak 3324, identified as either IAA or 5-HIAL, is a potential intermediate in Trp/IAA biosynthesis [63,64] and showed increased levels in RI. Additional increases were observed for nicotinic acid mononucleotide and 2,5-dihydroxypyridine (NaN metabolism), Tau, HTau, and aminoacetaldehyde (AALD; Tau/HTau metabolism), as well as for Phe and SA (Figure 4G,O,Q).
Several biosynthetic pathways were also activated in RI, including ACN (Figure 4C), GSL (Figure 4D), Mel (Figure 4F), Trop/Pid/pyridine (Figure 4L), and Phe/Tyr/Trp biosynthesis (Figure 4M). For example, five DAMs related to ACN biosynthesis, including pelargonidin 3-O-β-D-sambubioside, accumulated in RI at multiple time points (Figure 4C). GSL-related metabolites such as homo-Met, p-HPAH, Phe, Met, and Leu also increased in RI (Figure 4D).
In Mel biosynthesis, NAS and 5-MT were significantly increased at 11 dpi in RI (Figure 4F). Additionally, peak 3618—putatively matched to Mel in the Tier-3 library—increased by 130- and 389-fold in RI compared to RT at corresponding time points (Figure 4F). Other compounds, including 5-aminopentanal (Trop/Pid/pyridine biosynthesis), D-erythrose 4-phosphate, and 3-HPP (Phe/Tyr/Trp biosynthesis), were also strongly up-regulated in RI (Figure 4L,M).

2.5.5. Scopoletin/Isoscopoletin Biosynthesis

The highly induced metabolite E-6′-HF, along with ferulic acid (FA) and caffeic acid (CFA), are key intermediates in the scopoletin biosynthesis pathway and may also be involved in isoscopoletin production [65,66]. In canola, FA and CFA accumulated more strongly in RC than in other treatments (Figure 4E & Supplementary Figure S3E), whereas E-6′-HF showed a consistent and marked increase in RI at all time points (Figure 4E).

2.5.6. Redox Metabolism (GSH/GSSG)

Both reduced (GSH) and oxidized glutathione (GSSG) levels increased in response to infection (Figure 5A,B). GSH was higher in RI than TI at 7 dpi, whereas GSSG was higher in TI than RI at 11 dpi (Figure 5A). Despite this, the GSH/GSSG ratio—a key indicator of cellular redox balance [67,68]—remained consistently higher in RI than in TI from 3 to 11 dpi (Figure 5C), suggesting a more reduced cellular environment (less oxidative damage) in RI during infection.

2.5.7. Flavonoid-Related Pathways

Several DAMs involved in the biosynthesis of flavones/flavonols (Supplementary Figure S3A), flavonoids (Supplementary Figure S3B), phenylpropanoids (PPs; Supplementary Figure S3C), and in the metabolism of Gly, Ser, and Thr (Supplementary Figure S3D) were suppressed in RI. These included quercitrin, kaempferol, quercetin, luteolin, EC, and 5-aminolevulinic acid (Supplementary Figure S3A–D). In contrast, the isoflavonoid MG increased dramatically in RI, accumulating to levels 5- to 24-fold higher than in RC at 7 and 11 dpi (Figure 4I).

2.6. Validating DAM Candidates for Their Potential Roles in Rlm1-Mediated Resistance

Nine metabolites were selected and applied exogenously to the cotyledons of susceptible canola varieties to assess their roles in Rlm1-mediated resistance. When applied to Topas and Westar prior to inoculation, all metabolites—along with the phenylpropanoid pathway inhibitor PipA [69] (Table 4)—significantly reduced lesion development compared to controls (p < 0.05, LSD; Figure 6). While some metabolites showed slightly greater efficacy in one or both varieties, pipecolic acid (PA) consistently demonstrated the strongest suppression. Additionally, PA also reduced lesion expansion when applied post-inoculation at 1 and 3 dpi on both Topas and Westar but had no effect at 9 dpi (Figure 7).

3. Discussion

PCA analysis revealed distinct metabolomic patterns linked to Rlm1-mediated resistance, with RI (7 and 11 dpi) and TI (11 dpi) samples clustering separately (Figure S1), suggesting unique metabolic responses associated with Rlm1 and/or infection duration. Hierarchical clustering heatmaps further supported these distinctions (Figure 3). Several DAMs increased markedly in inoculated Topas–Rlm1 at 3 dpi and continued to accumulate at 7 and 11 dpi, whereas in inoculated Topas, similar changes were often delayed until 11 dpi. This earlier accumulation may reflect a role in Rlm1-mediated resistance.
During RI resistance, multiple pathways were activated, including those related to the biosynthesis of Lys, SA, GA, Met, GABA, GSH, and Mel, as well as Lys degradation (Figure 4). These pathways involved numerous DAMs and are consistent with previous studies linking these metabolites to plant defense. Their specific roles in disease resistance are discussed in the following sections.
Lysine and derivatives: Lys biosynthesis involves key intermediates, such as DAP and meso-DAP [70,71], while its degradation produces cadaverine, glutamic acid, AAA, D-1-P2C, SAP, and ultimately pipecolic acid (PA), a known non-protein amino acid involved in SAR [62,72,73,74]. In this study, both meso-DAP and DAP accumulated significantly in resistant interactions (RI), and PA levels rose 164- and 326-fold at 7 and 11 dpi, respectively, compared to resistant controls (RC) (Figure 4A,B). Lys catabolic intermediates such as SAP, AAA, Nε,Nε-dimethyllysine, N6-Acetyl-Lys, 5-hydroxylysine, and 5-APA also increased significantly in RI (Figure 4A). These findings align with prior studies demonstrating Lys’s role in SAR and its function as a precursor for alkaloid biosynthesis [32,33,75].
Beyond PA and AAA, we observed the accumulation of other amino acids, including GABA, Tyr, and Met—but not Trp—in RI (Figure 4H,P), suggesting broader amino acid pathway involvement in resistance. PA may also coordinate SAR with aspartate-derived amino acid homeostasis (Ile, Met, Thr, Lys) [33]. Similar elevations of PA, AAA, GABA, Tyr, Trp, Leu, Ile, and Lys were previously reported in resistant Arabidopsis and tobacco infected with Pseudomonas syringae [73,76]. Furthermore, in wheat, conversion of Lys into α-aminoadipate via the SAP pathway contributed to early resistance against Puccinia striiformis f. sp. tritici, marked by elevated 2-AAA and SAP [77,78].
Exogenous treatments of susceptible canola seedlings with PA, Lys, or DAP significantly limited infection on cotyledons (Figure 6 and Figure 7), supporting their roles in blackleg resistance. Even a quantitative reduction in infection development on cotyledons may restrict pathogen spread into the stem [6]. Notably, PA applications at 1 or 3 dpi also suppressed infection, while treatment at 9 dpi had no effect (Figure 7), indicating that early PA induction—likely during the biotrophic phase—is critical. Once the pathogen enters its necrotrophic phase, PA becomes less effective. This notion is supported by our observation that several DAMs accumulated equally or more in susceptible Topas than in resistant Topas–Rlm1 by 11 dpi (Figure 3), highlighting the importance of early metabolite induction in Rlm1-mediated resistance.
SA: Both free and bound SA levels were highly elevated in RI (Figure 4J), supporting SA’s central role in resistance [79,80,81,82]. Transcriptomic data from Zhai, Liu [17] similarly reported strong activation of SA signaling in Rlm1-mediated resistance. SA regulates pathogenesis-related (PR) genes, which encode antimicrobial proteins or amplify defense signaling [83,84,85,86]. Additionally, SA enhances plant defense by promoting callose deposition [87], ROS production [88], and programmed cell death [5,89,90,91].
GA: A secondary metabolite derived from SA [92], GA contributes to plant defense responses, though its role can vary with host–pathogen interactions [93,94]. In this study, both GA and SA significantly accumulated in the resistant interaction (RI) at 7 and 11 dpi, respectively (Figure 4J). Similar to SA, GA can act as a signaling molecule that induces antimicrobial PR proteins, such as P23, P32, and P34 in tomato [93].
However, unlike SA, GA accumulation has been reported primarily in compatible or non-necrotic interactions (e.g., ToMV, CEVd), but not in incompatible, HR-associated responses [93,95]. For example, in cucumber, low-dose P. syringae inoculation triggered GA accumulation during a compatible response, while high-dose inoculation led to HR-like necrosis without GA induction [40]. In contrast, our results show clear GA accumulation during an incompatible interaction with L. maculans, suggesting a distinct role for GA in Rlm1-mediated resistance in canola.
Exogenous application of GA or SA before inoculation significantly reduced disease symptoms in susceptible Topas (Figure 6), confirming their involvement in defense. SA peaked earlier than GA (7 vs. 11 dpi; Figure 4J), implying a more prominent role for SA during the early, biotrophic phase of infection, with GA potentially contributing during later stages.
Glucosinolates: Several GSL biosynthesis-related compounds, including p-HPAH, Met, and homo-Met, were significantly induced in inoculated Topas–Rlm1 (Figure 4D). GSL hydrolysis by myrosinase generates antimicrobial products such as isothiocyanates, thiocyanates, and nitriles [96,97,98,99,100]. Met and homo-Met are key intermediates in GSL biosynthesis [101,102,103], and Met also contributes to ethylene production and DNA methylation [104,105,106]. Previous studies showed that Met application or METS1 overexpression enhanced resistance to rice blast in rice [104,105]. The significant accumulation of Met, homo-Met, and p-HPAH during the incompatible interaction between Topas–Rlm1 and L. maculans suggests that the GSL-related pathway plays a role in the resistance.
NAS, Mel, and 5-MT: In Arabidopsis, rice, and cassava, NAS is a key precursor in Mel biosynthesis, converted to Mel via ASMT [107,108,109], and this activity enhances resistance to Xanthomonas axonopodis in benth and cassava [109]. Exogenous application of Mel, NAS, 5-MT, or 5-methoxyindole has similarly boosted resistance in Arabidopsis, tobacco, and benth [110,111]. In this study, RI samples showed elevated levels of NAS, 5-MT, and a metabolite (Peak 3618) tentatively identified as Mel (Figure 4F), suggesting potential coordinated up-regulation of Mel biosynthesis during Rlm1-mediated resistance.
GABA contributes to plant immunity by acting as a signaling molecule that regulates stress responses, inhibits pathogens directly, and modulates ROS to limit oxidative damage [112,113,114]. In cucumber, exogenous GABA boosts antioxidant enzyme activity, reducing H2O2 and superoxide levels [114]. It also mediates hormone crosstalk and activates induced systemic resistance (ISR) pathways [112]. In citrus, GABA treatment increased endogenous levels and induced defense-related hormones such as SA, CA, and ABA [115]. The elevated GABA observed in RI canola samples is consistent with its defense-associated roles in other plant–pathogen systems.
GSH, GSSG, and their ratio, along with antioxidant enzymes, are crucial redox components in plant defense against oxidative stress [68]. Exogenous SA enhances GSH levels in tomato [116] and stimulates GSH and chlorogenic acid accumulation in chickpea [117]. During hypersensitive response, GSH and tryptophan-derived metabolites limit pathogen growth in Arabidopsis [118]. In wheat, GSSG transport to the apoplast supports class III peroxidase activity, generating ROS for defense against Hessian fly larvae [67], and a high GSH/GSSG ratio is linked to powdery mildew resistance [119]. Consistent with these findings, our results showed induction of both GSH and GSSG in Topas and Topas–Rlm1 upon blackleg infection, with significantly higher GSH/GSSG ratios in Topas–Rlm1 (Figure 5), highlighting its role in canola defense.
E-6′-HF is a hydroxy-cinnamic acid (hydroxy-CA) phenolic compound [65], and hydroxy-CAs are well known for their antimicrobial and antioxidant activities [120]. Plants synthesize hydroxy-CA compounds in response to pathogen attack [121]. The accumulation of E-6′-HF, FA, and CFA in Topas–Rlm1 (Figure 4E & Supplementary Figure S3E) suggests their defensive roles. Phe, via the phenylpropanoid pathway, is a precursor for FA and CFA, which in turn contribute to E-6′-HF biosynthesis [65,66]. FA, derived from cinnamic acid (CA), activates the phenylpropanoid pathway and stimulates ROS production, enhancing plant defense [122]. This phenylpropanoid pathway also leads to the synthesis of SA, phytoalexins, and lignin, key components of plant immunity [123].
CFA derivatives reinforce plant cell walls and enhance defense, also exhibiting direct antimicrobial effects—for example, nanoparticle formulations with methyl-CFA and CFA-phenethyl-ester effectively target Ralstonia solanacearum [124,125,126]. Unexpectedly, inoculated Topas–Rlm1 showed no significant increase in Phe levels compared to susceptible Topas (Figure 4E and Figure S3C), despite Phe being the precursor for FA and CFA in the phenylpropanoid pathway and commonly associated with disease resistance [127,128,129]. This suggests that FA and CFA biosynthesis in Topas–Rlm1 may be regulated independently of Phe availability, potentially via pathway modulation during infection.
In this study, peak 3324 (Figure 4N) tentatively matched both IAA and 5-HIAL in the Tier 3 library, suggesting involvement in Trp metabolism and IAA biosynthesis [63,64]. While Trp is a known precursor for several defense-related metabolites [63,64], its levels did not increase in inoculated Topas–Rlm1. Instead, peak 3324, 2-AA, 2-AP, and 5-HKA accumulated at 7 and/or 11 dpi (Figure 4N). 2-AA, a key IAA intermediate, is also linked to SA-dependent ISR and PR gene activation [130,131,132,133], while 2-AP and 2-AA have antimicrobial properties [134]. Notably, 5-HKA has been associated with resistance in soybean roots during Phytophthora sojae infection [135], supporting its potential role in canola defense.
Several metabolites linked to ACN biosynthesis and plant defense accumulated significantly in inoculated Topas–Rlm1 plants (Figure 4C,I,K), though their specific roles in resistance remain unclear. In grapevine, ACN pathway metabolites have been associated with SAR against Botrytis cinerea [136]. Notably, NCP, a polyamine biosynthesis intermediate [137,138], accumulated early in Topas–Rlm1 but increased more slowly than in Topas (Figure 4K). NCP contributes to spermine production [137,138], which enhances resistance by triggering HR and SA-independent PR proteins [139,140]. Additionally, the isoflavonoid MG increased in Topas–Rlm1 (Figure 4I), consistent with its role in soybean resistance to Euschistus heros [52], suggesting a potential defense function in canola.
Exogenous application of selected DAMs—including PA, FA, CFA, SA, GA, DAP, GSH, BA, and Lys, as well as the inhibitor of the phenylpropanoid pathway PipA [69]—significantly reduced infection in susceptible Topas and/or Westar (Figure 6 and Figure 7), confirming their roles in blackleg resistance. Repeated applications before inoculation likely maximized treatment effects, while early post-inoculation application of PA also proved effective, emphasizing the importance of early activation of these metabolites (Figure 7).
Several DAMs with known or potential antimicrobial activity were enriched in inoculated Topas–Rlm1 plants, though their timing varied (Figure 4K). Medicarpin, PZD, and mangiferin increased early (3 dpi), while 3-HMA, EMD, and PG accumulated later. Medicarpin, a phytoalexin, activates SA-related defense pathways and has been linked to powdery mildew resistance in Medicago truncatula [45,46]. Mangiferin, PZD, and PG derivatives also exhibit antimicrobial properties, with mangiferin shown to inhibit Fusarium oxysporum in vitro and in planta [47,48,49]. Similarly, 3-HMA suppresses spore germination and hyphal growth of F. oxysporum [50]. EMD, which modestly increased at 7 dpi, has been associated with phytoalexin induction and HR [51]. While these compounds may contribute to defense, their roles in Rlm1-mediated resistance have not been confirmed.
Conversely, several DAMs involved in phenylpropanoid, flavonoid, and Gly/Ser/Thr metabolism showed reduced accumulation in Rlm1 plants (Figure 4Q and Figure S3). Although linked to resistance or stress tolerance in other species [58,141,142,143], their down-regulation here suggests that these metabolites may be non-essential or even detrimental in the context of blackleg resistance. Some phenolic compounds can facilitate pathogen colonization [144], and selective suppression of these may prevent exploitation by the pathogen. Metabolic flux from the phenylpropanoid pathway can also be redirected toward SA biosynthesis [145,146], a key defense signal [147]. Supporting this, treatment with PipA—a phenylpropanoid pathway inhibitor [69]—reduced disease symptoms in susceptible Topas (Figure 7B), suggesting that blocking certain branches enhances SA-mediated resistance [69,145,146].
While this study provides valuable insights into metabolite profiles associated with Rlm1-mediated resistance to L. maculans, several limitations should be noted. First, the untargeted metabolomics approach enabled broad DAM identification, but definitive confirmation—particularly of Tier-3 compounds—was limited by the absence of authentic standards or MS/MS data. Second, the controlled growth-incubator conditions may not fully replicate field environments, where abiotic stressors, soil microbiota, and variable pathogen pressures could influence metabolite responses. Third, although some DAMs suppressed infection when applied exogenously, the underlying mechanisms remain undetermined, and such applications may not reflect endogenous biosynthesis or localization exactly. Lastly, the exclusive use of Topas and Topas–Rlm1 may not capture responses mediated by additional R genes present in commercial cultivars.
Despite these constraints, this study offers a foundation for future research. Targeted metabolomics with authentic standards and MS/MS validation should be used to confirm and quantify key DAMs, particularly those putatively annotated but shown to have strong associations with resistance, such as NAS, Mel, 5-MT, and 2-AA. Integrating transcriptomic and proteomic data will help elucidate gene–metabolite networks and regulatory mechanisms. Functional validation of candidate biosynthetic genes through CRISPR/Cas9, via overexpression or knockout, can establish causal links to resistance. Finally, extending analyses to other R genes or QTLs may uncover both gene-specific and broad-spectrum metabolic signatures, advancing efforts toward durable blackleg resistance.

4. Materials and Methods

4.1. Plant Materials and Pathogen Isolates

A near isogenic line (NIL) of B. napus carrying the R gene Rlm1 was developed at the Saskatoon Research and Development Center, AAFC [148]. The process used the ‘Quinta’ double haploid (DH) line DH24288, which carries Rlm1 and Rlm3 [149], as the R parent in backcrossing with the susceptible Topas DH16516 [148]. The NIL and Topas DH16516 were used as resistant and control lines, respectively, throughout the study. Each line was planted in 128-well flats filled with Sunshine #3 soilless mix (Sun Gro Hort. Canada Ltd., Vancouver, BC, Canada) amended with 12.5 g L−1 Osmocote Plus 16-9-12 (N-P-K, Scotts Miracle-Gro Canada Ltd., Mississauga, ON, Canada). Seeded flats were placed in an incubator set with a day/night temperature regime of 22/18 °C, about 65% relative humidity, and a daily photoperiod (427 µmol·m−2·s−1) of 16 h. In later experiments, to validate the effect of putative metabolites identified on blackleg resistance, a DH line of ‘Westar’ [150,151] was planted similarly as an additional susceptible control.
The L. maculans isolate Sc006 carrying the avirulence gene AvrLm1 was used to inoculate all plants. The isolate was cultured on V8-juice agar amended with streptomycin sulfate (100 ppm) at 20 °C under cool-white fluorescent light (325 µmol·m−2·s−1) for 7–10 d for inoculum production [152]. Pycnidiospores were harvested by flooding the culture plates with sterilized water and filtering resulting spore suspension through a Falcon™ Cell Strainer (70 μm, Corning/Sigma-Aldrich, Markham, ON, Canada). The concentration of the obtained spore suspension was estimated using a hemocytometer and adjusted to 2 × 107 spores/mL with sterilized water for plant inoculation.

4.2. Plant Inoculation, Infection Assessment, and Leaf-Tissue Sampling

About a week after seedling emergence, each lobe of cotyledon was pricked with a pair of bent-tipped tweezers, and each wound was inoculated with a 10-μL droplet of prepared spore suspension. Wounds receiving sterilized water were used as non-inoculated controls (mock). Inoculated seedlings were air dried at room temperature for 30 min before being placed back in the incubator. Following the inoculation, emerging true leaves were removed every 3–5 days to delay the senescence of inoculated cotyledons. At 12–14 days post-inoculation (dpi), the severity of infection on inoculated and control cotyledons was assessed using a 0–9 scale introduced by Koch, Badawy [153], with cotyledon tissues immediately around the inoculation site or expanding lesion being sampled using a paper puncher at 3, 7, and 11 dpi, respectively. This hemibiotrophic fungus tends to establish a transient biotrophic relationship with the host following successful infection (3 dpi) before transitioning to the necrotrophic phase around 7–10 dpi, when visible necrotic lesions begin to form [17,154]. Samples from 20 random seedlings of the same treatment were bulked and grounded in liquid nitrogen into a fine powder using a mortar and pestle to form a biological replicate, with three replicates prepared for each treatment or control at each of the time points of sampling. All bulked samples were stored at −80 °C until use.

4.3. Sample Preparation for Metabolomic Analysis Using CIL LC–MS

Bulked cotyledon samples were extracted for metabolites following the protocol described by Tunsagool, Wang [31] at the Metabolomics Innovation Centre (TMIC), University of Alberta, with only slight modifications. Chemicals and reagents were sourced from Sigma-Aldrich (Markham, ON, Canada) unless indicated otherwise. Briefly, 300 mg of each sample was transferred into a 2 mL Eppendorf tube containing 1.5 mL of extraction buffer (methanol/water, 4:1, v/v) and 2.8 mm ceramic beads. After vortexing for 15 s, the sample was homogenized using the Bioprep-24 homogenizer (Allsheng, Hangzhou, China) for another 15 s. The tube was then placed in a −20 °C freezer for 10 min before being centrifuged at 15,000× g at 4 °C for 10 min (Eppendorf 5430R, Mississauga, ON, Canada). The resulting supernatant was transferred to a fresh vial for subsequent metabolomic analysis.
Dansylation labeling, qualification, and LC–MS analysis of samples generally followed the protocol described previously by Luo, Zhao [29]; 25 µL of each extraction was dried under nitrogen blowdown and reconstituted in an equal volume of LC–MS grade water (Canadian Life Sciences, Peterborough, ON, Canada). The samples were then processed following the SOPs provided in the kit by the manufacturer of Dansyl-labeling Kit for Amine & Phenol Metabolomics (NMT-4101-KT, Nova Medical Testing Inc., Edmonton, AB, Canada). Each sample was labeled using 12C-labeling reagent. To establish a reference standard, a pooled sample was produced by mixing an equal amount of extraction from each individual sample, labeled with a 13C-labeling agent, as a baseline for metabolomic analysis [29].

4.4. Metabolome Quantification

An LC–UV-based standard operating protocol (SOP) established at TMIC was used to quantify labeled metabolites for sample amount normalization [155]. All dansylation-labeled samples were centrifuged at 15,000× g for 10 min, with 25 µL of each supernatant being used for UV quantification in an HPLC vial (Agilent, Boulder, CO, USA). Each 12C-labeled sample was mixed with an equal molar amount of the 13C-labeled pooled sample based on the SOP. This mixture was measured using LC–MS analysis for the peak intensity ratio of metabolites between individual samples and the pooled sample [29]. Quality control (QC) over the LC–MS analysis was performed by combining equal amounts of 12C-labeled and 13C-labeled pools.

4.5. LC−MS Analysis

LC–MS analysis of labeled metabolite samples was conducted following the previously reported method [42] at TIMC, utilizing a Thermo Vanquish UHPLC system coupled to a Q Exactive Orbitrap mass spectrometer (Thermo Scientific, Edmonton, AB, Canada). To ensure consistent instrument performance, quality control (QC) samples were injected after every 20 sample runs. Mixed 12C- and 13C-labeled samples were separated using an Eclipse Plus C18 reversed-phase column (2.1 mm × 15 cm, 1.8 μm particle size; Agilent Technologies., Mississauga, ON, Canada), with mobile phases and gradient conditions adapted from Luo, Zhao [29]. Solvent A consisted of 0.1% (v/v) formic acid in water, and solvent B was 0.1% (v/v) formic acid in acetonitrile. The gradient program was 25% B at 0 min, ramped to 99% B by 10 min, held at 99% B until 13 min, returned to 25% B at 13.1 min, and maintained until 16 min. MS conditions were as follows: flow rate of 400 μL/min, column temperature at 40 °C, mass range m/z 220–1000, and acquisition rate of 1 Hz. The gradient and mass range were adopted from a well-established protocol specifically for chemical isotope labeling LC–MS metabolomics [42], which optimize LC–MS parameters for metabolites labeled with dansyl chloride by increasing both mass and hydrophobicity of metabolites.

4.6. LC–MS Raw Data Extraction and Processing

The open-source ProteoWizard MSConvert software v 2.1 (https://proteowizard.sourceforge.io/; accessed on 2 July 2022) was used to convert all LC–MS raw data from profile mode into .txt files [156], and a suite of programs developed at TIMC was employed to process the converted data in batch mode [157]. IsoMS Pro 1.2.5 software [157] was used to pick and align peak pairs and calculate the intensity ratio of metabolites. Peak pairs that were not present in at least 80.0% of samples in any group were filtered out. Data were then normalized by Ratio of Total Useful Signal, calculated as the sum of 12C-labeled peak signals divided by the sum of 13C-labeled peak signals [158]. This ratio served as a post-acquisition normalization method [158].
For metabolite identification, three-tiered databases developed at TMIC were utilized, including the labeled standard database (CIL Library) as Tier-1 [41], the linked identity library (LI Library) as Tier-2 [42], and MyCompoundID library as Tier-3 [43]. Tier-1 database provided positive identification results by matching to experiential information of compound standard, with high-confidence identification. Tier-2 delivered high-confidence putative identification results based on both experimental and predicted information. Tier-3 generated putative results in which peaks with multiple matches would not be considered. For compounds with multiple matching peaks, only the peak with the lowest absolute error in mass and retention time was retained for analysis. Extracted LC–MS peak data are presented in Supplementary Table S1.

4.7. Validating the Potential Involvement of Selected Metabolites in Resistance

4.7.1. Chemical (Metabolite) Preparation

Table 4 lists the most prominent DAMs and related pathways identified in inoculated Rlm1 plants, including PA, SA, GA, GSH, Lys, and DAP. Several DAMs found in non-inoculated Rlm1 plants, including FA and CFA, were also selected for functional validation due to their high accumulations. The ferulic acid E-6′-HF, along with FA and CFA, are involved in scopoletin (SCF)/isoscopoletin (IsoScp) biosynthesis [65,66]. However, E-6′-HF is unavailable commercially; therefore, only CFA and trans-FA (trans-ferulic acid, a ferulate isomer in plants) were tested in this experiment. Although BA (peak 1365) was only tentatively identified based on Tier-3 database annotation, it was selected for this investigation due to its notably high levels in non-inoculated Rlm1 plants. PipA is a well-known inhibitor of the phenylpropanoid pathway in plants [69], and may play a role in this context, as several DAMs associated with this pathway were suppressed during Rlm1-mediated incompatible interaction. However, for most of these metabolites, their roles in blackleg resistance remain uncharacterized and have yet to be experimentally validated.
Most of the selected metabolites could be dissolved in water, while FA and PipA had to be dissolved first in methyl sulfoxide (DMSO, Sigma-Aldrich Canada, Oakville, ON, Canada) before being diluted to desired concentrations using deionized water. The final solution of FA and PipA contained 0.17% and 1.7% of DMSO, both at low enough concentrations to have minimum effects on canola seedlings. CFA and BA were dissolved initially in 95% ethanol, then diluted with water to achieve desired concentrations in which the ethanol content was 9.5% and 6.8%, respectively. All final metabolite preparations were amended with Triton X-100 (surfactant) at 0.01% for improved spreadability and adherence during spray applications. Water, 0.17%/1.7% of DMSO, or 9.5%/6.8% of ethanol amended with the surfactant was used as a control depending on the treatment.

4.7.2. Application of Metabolites

Two canola varieties, Topas (susceptible) and Westar (highly susceptible), were used to validate the effects of the metabolites. These DAM preparations were applied to canola seedlings using a misting bottle (Uline, Milton, ON, Canada) twice daily, with approximately 0.3 mL per seedling per application, starting two days after emergence and continuing for five consecutive days until the day of inoculation. In addition to the pre-inoculation effect, PA was also studied for its post-inoculation effect due to its highest efficacy of infection suppression observed, with twice-daily spray applications at 1, 3, and 9 dpi.

4.7.3. Inoculation and Infection Assessment

About one hour after the final DAM treatment, each cotyledon lobe was inoculated with the highly virulent L. maculans isolate (Sc006) and the infection development was assessed using the 0–9 scale at 14 dpi [153], as described above. Inoculated cotyledons sprayed with water, or with the corresponding low concentrations of DMSO or ethanol solution at matched time points, served as controls (Table 4). The experiments followed a completely randomized design (CRD). All metabolites were evaluated in 2–3 independent trials, with 4 to 24 biological replicates (plants) per treatment per trial, depending on the metabolite and trial.

4.8. Data Analysis

Most data analyses were performed using R (ver.4.3.3) [159] and RStudio (ver.2023.12.1) [160]. Metabolomics data were processed initially with a 104 integer transformation prior to normalization. Multivariate and univariate analyses were performed using the DESeq2 package [161], which applies the Benjamini–Hochberg method to correct for multiple testing, to explore overall data structure/patterns/group separation across samples (multivariate), and to identify individual metabolites that significantly contribute to these differences (univariate). This integrated approach allows us to gain both global (pattern-level) and specific (feature-level) understanding of the data. Fold changes (FC) were calculated for sample pairs at 3, 7, and 11 dpi. Metabolites were classified as differentially accumulated if log2FC ≥|1| and false discovery rate (FDR)–adjusted p ≤ 0.05. Principal component analysis (PCA) was carried out with the “prcomp” function from the base R package [159] and visualized with the “ggplot2” package [162,163,164]. Venn diagrams were created using the “ggvenn” package [165] to illustrate the overlap or uniqueness of metabolites among different treatment groups or time points, and heatmap analysis was performed with the “pheatmap” package [166] to provide a detailed visualization of the abundance levels of metabolites across samples or conditions.
Additionally, the relative intensities of BA, GSH, and oxidized glutathione (GSSG) in the LC–MS chromatogram, as well as the ratio of GSH/GSSG readings, were analyzed initially with ANOVA using “rstatix” [167]; a reduced ratio is a key indicator of cellular redox balance, which is critically relevant to plant disease resistance [68]. The post hoc examination of treatment means used LSD in the “agricolae” [168], visualized with “ggplot2” [162,163,164]. Pathway analysis of highly regulated DAMs was performed using the Arabidopsis metabolite database in MetaboAnalyst6.0 [44].
All data on infection severity of inoculated cotyledons from repeated trials were pooled due to general homogeneity of variance. Pooled data were analyzed for normality (Shapiro–Wilk test) prior to ANOVA, followed with LSD post hoc analysis for mean separation when ANOVA showed significance (p ≤ 0.05). For data that lacked a normal distribution, aligned-ranks transformation ANOVA (ART ANOVA) was performed using the “ARTool” package [169,170,171,172], followed with a post hoc test using “emmeans” [173]. Significance groupings were labeled using the “rcompanion” package based on p-values [170,174].

5. Conclusions

Using the susceptible Topas and its isogenic Rlm1-carrying resistant counterpart, combined with the CIL LC–MS platform for metabolomic analysis, we identified distinct metabolic profiles associated with compatible and incompatible interactions during infection by L. maculans. These differences were most pronounced at specific post-inoculation time points. Our hypotheses regarding the metabolic divergence between susceptible and resistant canola—and the suitability of the CIL LC–MS platform for this investigation—were largely validated. As a result, our objectives were successfully met.
In Topas–Rlm1, several key defense-related metabolites were strongly and consistently up-regulated, most notably PA, which increased by up to 326-fold, underscoring its potential central role in resistance. Elevated levels of SA, its derivative GA, glucosinolate pathway intermediates, and GABA further suggest a coordinated hormonal and antimicrobial response. Conversely, lower activity in flavonoid and amino acid metabolism in the resistant line implies these pathways may be less critical to Rlm1-mediated defense. Moreover, exogenous application of several defense-associated metabolites—including PA, SA, and GA—significantly reduced infection in susceptible canola varieties, supporting their functional role in blackleg resistance. Overall, this study highlights key metabolic components underpinning Rlm1-mediated resistance and demonstrates the potential of metabolite-based approaches—including metabolic engineering—as promising strategies for enhancing blackleg resistance in canola.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/ijms26125627/s1.

Author Contributions

This study was conceived by G.P. and X.Z., and directed by G.P. and L.L. (metabolomics). X.Z. carried out biological assays, while S.Z. and X.L. performed metabolomic assays, including sample preparation, LC–MS, and initial data analysis. P.G. and X.Z. performed bioinformatics. X.Z. wrote the original draft, and G.P. edited the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This research was supported financially by the Canola Council of Canada through the joint funding initiative with Agriculture and Agri-Food Canada under Canola AgriScience Cluster: Sustainable, Reliable Supply for a Changing World (Project Funding Number: ASC-02, Activity 27, 2018–2023).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The metabolomics data have been deposited to the MetaboLights [175] with the study identifier MTBLS12542 (https://www.ebi.ac.uk/metabolights/MTBLS12542; deposited on 30 May 2025), following Nature’s Data Repository Guidance.

Conflicts of Interest

The authors declare that they have no competing financial interests or personal relationships that could have compromised the work reported in this manuscript.

References

  1. Statistics Canada. Production of Principal Field Crops, November 2024. The Daily, 5 December 2024. Available online: https://www150.statcan.gc.ca/n1/daily-quotidien/241205/dq241205b-eng.htm (accessed on 31 March 2025).
  2. Chen, G.; Wu, C.; Li, B.; Su, H.; Zhen, S.; An, Y. Detection of Leptosphaeria maculans from imported Canola seeds/Nachweis von Leptosphaeria maculons in importiertem Rapssaatgut. J. Plant Dis. Prot. 2010, 117, 173–176. [Google Scholar] [CrossRef]
  3. Fitt, B.D.; Hu, B.; Li, Z.; Liu, S.; Lange, R.; Kharbanda, P.; Butterworth, M.; White, R. Strategies to prevent spread of Leptosphaeria maculans (phoma stem canker) onto oilseed rape crops in China; costs and benefits. Plant Pathol. 2008, 57, 652–664. [Google Scholar] [CrossRef]
  4. Zhang, X.; Peng, G.; Kutcher, H.R.; Balesdent, M.-H.; Delourme, R.; Fernando, W.D. Breakdown of Rlm3 resistance in the Brassica napus–Leptosphaeria maculans pathosystem in western Canada. Eur. J. Plant Pathol. 2016, 145, 659–674. [Google Scholar] [CrossRef]
  5. Hubbard, M.; Zhai, C.; Peng, G. Exploring mechanisms of quantitative resistance to Leptosphaeria maculans (Blackleg) in the cotyledons of canola (Brassica napus) based on transcriptomic and microscopic analyses. Plants 2020, 9, 864. [Google Scholar] [CrossRef]
  6. Hubbard, M.; Peng, G. Quantitative resistance against an isolate of Leptosphaeria maculans (blackleg) in selected Canadian canola cultivars remains effective under increased temperatures. Plant Pathol. 2018, 67, 1329–1338. [Google Scholar] [CrossRef]
  7. Balesdent, M.-H.; Gautier, A.; Plissonneau, C.; Le Meur, L.; Loiseau, A.; Leflon, M.; Carpezat, J.; Pinochet, X.; Rouxel, T. Twenty years of Leptosphaeria maculans population survey in France suggests pyramiding Rlm3 and Rlm7 in rapeseed is a risky resistance management strategy. Phytopathology® 2022, 112, 2126–2137. [Google Scholar] [CrossRef]
  8. Liban, S.; Cross, D.; Kutcher, H.; Peng, G.; Fernando, W. Race structure and frequency of avirulence genes in the western Canadian Leptosphaeria maculans pathogen population, the causal agent of blackleg in brassica species. Plant Pathol. 2016, 65, 1161–1169. [Google Scholar] [CrossRef]
  9. Soomro, W.; Kutcher, R.; Yu, F.; Hwang, S.-F.; Fernando, D.; Strelkov, S.E.; Peng, G. The race structure of Leptosphaeria maculans in western Canada between 2012 and 2014 and its influence on blackleg of canola. Can. J. Plant Pathol. 2021, 43, 480–493. [Google Scholar] [CrossRef]
  10. Liu, F.; Zou, Z.; Peng, G.; Dilantha Fernando, W. Leptosphaeria maculans isolates reveal their allele frequency in Western Canada. Plant Dis. 2021, 105, 1440–1447. [Google Scholar] [CrossRef]
  11. Rouxel, T.; Balesdent, M. The stem canker (blackleg) fungus, Leptosphaeria maculans, enters the genomic era. Mol. Plant Pathol. 2005, 6, 225–241. [Google Scholar] [CrossRef]
  12. Rouxel, T.; Penaud, A.; Pinochet, X.; Brun, H.; Gout, L.; Delourme, R.; Schmit, J.; Balesdent, M.-H. A 10-year survey of populations of Leptosphaeria maculans in France indicates a rapid adaptation towards the Rlm1 resistance gene of oilseed rape. Eur. J. Plant Pathol. 2003, 109, 871–881. [Google Scholar] [CrossRef]
  13. Borhan, M.H.; Van de Wouw, A.P.; Larkan, N.J. Molecular interactions between Leptosphaeria maculans and Brassica species. Annu. Rev. Phytopathol. 2022, 60, 237–257. [Google Scholar] [CrossRef]
  14. Chu, M.; Song, T.; Falk, K.C.; Zhang, X.; Liu, X.; Chang, A.; Lahlali, R.; McGregor, L.; Gossen, B.D.; Yu, F. Fine mapping of Rcr1 and analyses of its effect on transcriptome patterns during infection by Plasmodiophora brassicae. BMC Genom. 2014, 15, 1166. [Google Scholar] [CrossRef] [PubMed]
  15. Song, T.; Chu, M.; Lahlali, R.; Yu, F.; Peng, G. Shotgun label-free proteomic analysis of clubroot (Plasmodiophora brassicae) resistance conferred by the gene Rcr1 in Brassica rapa. Front. Plant Sci. 2016, 7, 1013. [Google Scholar] [CrossRef] [PubMed]
  16. Fudal, I.; Ross, S.; Gout, L.; Blaise, F.; Kuhn, M.; Eckert, M.; Cattolico, L.; Bernard-Samain, S.; Balesdent, M.; Rouxel, T. Heterochromatin-like regions as ecological niches for avirulence genes in the Leptosphaeria maculans genome: Map-based cloning of AvrLm6. Mol. Plant-Microbe Interact. 2007, 20, 459–470. [Google Scholar] [CrossRef]
  17. Zhai, C.; Liu, X.; Song, T.; Yu, F.; Peng, G. Genome-wide transcriptome reveals mechanisms underlying Rlm1-mediated blackleg resistance on canola. Sci. Rep. 2021, 11, 4407. [Google Scholar] [CrossRef]
  18. Jones, J.D.; Dangl, J.L. The plant immune system. Nature 2006, 444, 323–329. [Google Scholar] [CrossRef]
  19. AbuQamar, S.; Moustafa, K.; Tran, L.S. Mechanisms and strategies of plant defense against Botrytis cinerea. Crit. Rev. Biotechnol. 2017, 37, 262–274. [Google Scholar] [CrossRef]
  20. Bezerra-Neto, J.P.; Araújo, F.C.; Ferreira-Neto, J.R.; Silva, R.L.; Borges, A.N.; Matos, M.K.; Silva, J.B.; Silva, M.D.; Kido, E.A.; Benko-Iseppon, A.M. NBS-LRR genes—Plant health sentinels: Structure, roles, evolution and biotechnological applications. In Applied Plant Biotechnology for Improving Resistance to Biotic Stress; Elsevier: Amsterdam, The Netherlands, 2020; pp. 63–120. [Google Scholar]
  21. Wang, J.W.; Wu, J.Y. Effective elicitors and process strategies for enhancement of secondary metabolite production in hairy root cultures. In Biotechnology of Hairy Root Systems; Springer: Berlin/Heidelberg, Germany, 2013; pp. 55–89. [Google Scholar]
  22. Lobo, M.; Hounsome, N.; Hounsome, B. Biochemistry of vegetables: Secondary metabolites in vegetables—Terpenoids, phenolics, alkaloids, and sulfur-containing compounds. In Handbook of Vegetables and Vegetable Processing; John Wiley & Sons Ltd.: Hoboken, NJ, USA, 2018; pp. 47–82. [Google Scholar]
  23. Kumar, A.; Irchhaiya, R.; Yadav, A.; Gupta, N.; Kumar, S.; Gupta, N.; Kumar, S.; Yadav, V.; Prakash, A.; Gurjar, H. Metabolites in plants and its classification. World J. Pharm. Pharm. Sci. 2015, 4, 287–305. [Google Scholar]
  24. Pusztahelyi, T.; Holb, I.J.; Pócsi, I. Secondary metabolites in fungus-plant interactions. Front. Plant Sci. 2015, 6, 573. [Google Scholar] [CrossRef]
  25. Wink, M. Plant breeding: Importance of plant secondary metabolites for protection against pathogens and herbivores. Theor. Appl. Genet. 1988, 75, 225–233. [Google Scholar] [CrossRef]
  26. Cevallos-Cevallos, J.M.; Reyes-De-Corcuera, J.I. Metabolomics in food science. In Advances in Food and Nutrition Research; Elsevier: Amsterdam, The Netherlands, 2012; Volume 67, pp. 1–24. [Google Scholar]
  27. Luo, X.; Gu, X.; Li, L. Development of a simple and efficient method of harvesting and lysing adherent mammalian cells for chemical isotope labeling LC-MS-based cellular metabolomics. Anal. Chim. Acta 2018, 1037, 97–106. [Google Scholar] [CrossRef] [PubMed]
  28. Guo, K.; Li, L. Differential 12C-/13C-isotope dansylation labeling and fast liquid chromatography/mass spectrometry for absolute and relative quantification of the metabolome. Anal. Chem. 2009, 81, 3919–3932. [Google Scholar] [CrossRef] [PubMed]
  29. Luo, X.; Zhao, S.; Huan, T.; Sun, D.; Friis, R.M.N.; Schultz, M.C.; Li, L. High-performance chemical isotope labeling liquid chromatography–mass spectrometry for profiling the metabolomic reprogramming elicited by ammonium limitation in yeast. J. Proteome Res. 2016, 15, 1602–1612. [Google Scholar] [CrossRef]
  30. Shen, W.; Han, W.; Li, Y.; Meng, Z.; Cai, L.; Li, L. Development of chemical isotope labeling liquid chromatography mass spectrometry for silkworm hemolymph metabolomics. Anal. Chim. Acta 2016, 942, 1–11. [Google Scholar] [CrossRef]
  31. Tunsagool, P.; Wang, X.; Leelasuphakul, W.; Jutidamrongphan, W.; Phaonakrop, N.; Jaresitthikunchai, J.; Roytrakul, S.; Chen, G.; Li, L. Metabolomic study of stress responses leading to plant resistance in mandarin fruit mediated by preventive applications of Bacillus subtilis cyclic lipopeptides. Postharvest Biol. Technol. 2019, 156, 110946. [Google Scholar] [CrossRef]
  32. Liu, Y.; Li, Y.; Bi, Y.; Jiang, Q.; Mao, R.; Liu, Z.; Huang, Y.; Zhang, M.; Prusky, D.B. Induction of defense response against Alternaria rot in Zaosu pear fruit by exogenous L-lysine through regulating ROS metabolism and activating defense-related proteins. Postharvest Biol. Technol. 2021, 179, 111567. [Google Scholar] [CrossRef]
  33. Yang, H.; Ludewig, U. Lysine catabolism, amino acid transport, and systemic acquired resistance: What is the link? Plant Signal. Behav. 2014, 9, e28933. [Google Scholar] [CrossRef]
  34. Shan, L.; He, P. Pipped at the post: Pipecolic acid derivative identified as SAR regulator. Cell 2018, 173, 286–287. [Google Scholar] [CrossRef]
  35. El-Shetehy, M.; Wang, C.; Shine, M.; Yu, K.; Kachroo, A.; Kachroo, P. Nitric oxide and reactive oxygen species are required for systemic acquired resistance in plants. Plant Signal. Behav. 2015, 10, e998544. [Google Scholar] [CrossRef]
  36. Ghosh, S.; Kanwar, P.; Jha, G. Alterations in rice chloroplast integrity, photosynthesis and metabolome associated with pathogenesis of Rhizoctonia solani. Sci. Rep. 2017, 7, 41610. [Google Scholar] [CrossRef] [PubMed]
  37. Deng, X.; Xu, X.; Liu, Y.; Zhang, Y.; Yang, L.; Zhang, S.; Xu, J. Induction of γ-aminobutyric acid plays a positive role to Arabidopsis resistance against Pseudomonas syringae. J. Integr. Plant Biol. 2020, 62, 1797–1812. [Google Scholar] [CrossRef]
  38. Rani, M.; Jha, G. Host gamma-aminobutyric acid metabolic pathway is involved in resistance against Rhizoctonia solani. Phytopathol® 2021, 111, 1207–1218. [Google Scholar] [CrossRef]
  39. Mäkinen, K.; De, S. The significance of methionine cycle enzymes in plant virus infections. Curr. Opin. Plant Biol. 2019, 50, 67–75. [Google Scholar] [CrossRef]
  40. Bellés, J.M.; Garro, R.; Pallás, V.; Fayos, J.; Rodrigo, I.; Conejero, V. Accumulation of gentisic acid as associated with systemic infections but not with the hypersensitive response in plant-pathogen interactions. Planta 2006, 223, 500–511. [Google Scholar] [CrossRef]
  41. Cheng, Z.; Li, L. Development of Chemical Isotope Labeling Liquid Chromatography Orbitrap Mass Spectrometry for Comprehensive Analysis of Dipeptides. Anal. Chem. 2023, 95, 6629–6636. [Google Scholar] [CrossRef]
  42. Zhao, S.; Li, H.; Han, W.; Chan, W.; Li, L. Metabolomic coverage of chemical-group-submetabolome analysis: Group classification and four-channel chemical isotope labeling LC-MS. Anal. Chem. 2019, 91, 12108–12115. [Google Scholar] [CrossRef]
  43. Li, L.; Li, R.; Zhou, J.; Zuniga, A.; Stanislaus, A.E.; Wu, Y.; Huan, T.; Zheng, J.; Shi, Y.; Wishart, D.S. MyCompoundID: Using an evidence-based metabolome library for metabolite identification. Anal. Chem. 2013, 85, 3401–3408. [Google Scholar] [CrossRef]
  44. Pang, Z.; Lu, Y.; Zhou, G.; Hui, F.; Xu, L.; Viau, C.; Spigelman, A.F.; MacDonald, P.E.; Wishart, D.S.; Li, S. MetaboAnalyst 6.0: Towards a unified platform for metabolomics data processing, analysis and interpretation. Nucleic Acids Res. 2024, 52, gkae253. [Google Scholar] [CrossRef]
  45. Stevenson, P.; Turner, H.; Haware, M. Phytoalexin accumulation in the roots of chickpea (Cicer arietinum L.) seedlings associated with resistance to fusarium wilt (Fusarium oxysporum f. sp. ciceri). Physiol. Mol. Plant Pathol. 1997, 50, 167–178. [Google Scholar] [CrossRef]
  46. Gupta, A.; Awasthi, P.; Sharma, N.; Parveen, S.; Vats, R.P.; Singh, N.; Kumar, Y.; Goel, A.; Chandran, D. Medicarpin confers powdery mildew resistance in Medicago truncatula and activates the salicylic acid signalling pathway. Mol. Plant Pathol. 2022, 23, 966–983. [Google Scholar] [CrossRef] [PubMed]
  47. Kumar, R.S.; Moydeen, M.; Al-Deyab, S.S.; Manilal, A.; Idhayadhulla, A. Synthesis of new morpholine-connected pyrazolidine derivatives and their antimicrobial, antioxidant, and cytotoxic activities. Bioorg. Med. Chem. Lett. 2017, 27, 66–71. [Google Scholar] [CrossRef] [PubMed]
  48. Jyotshna; Khare, P.; Shanker, K. Mangiferin: A review of sources and interventions for biological activities. Biofactors 2016, 42, 504–514. [Google Scholar] [CrossRef] [PubMed]
  49. Ghosal, S.; Biswas, K.; Chakrabarti, D.K.; Basu Chaudhary, K. Control of Fusarium wilt of safflower by mangiferin. Phytopathology 1977, 67, 548–550. [Google Scholar] [CrossRef]
  50. Gao, X.; Li, K.; Ma, Z.; Zou, H.; Jin, H.; Wang, J. Cucumber Fusarium wilt resistance induced by intercropping with celery differs from that induced by the cucumber genotype and is related to sulfur-containing allelochemicals. Sci. Hortic. 2020, 271, 109475. [Google Scholar] [CrossRef]
  51. Godard, S.; Slacanin, I.; Viret, O.; Gindro, K. Induction of defence mechanisms in grapevine leaves by emodin-and anthraquinone-rich plant extracts and their conferred resistance to downy mildew. Plant Physiol. Biochem. 2009, 47, 827–837. [Google Scholar] [CrossRef]
  52. da Graça, J.P.; Ueda, T.E.; Janegitz, T.; Vieira, S.S.; Salvador, M.C.; de Oliveira, M.C.; Zingaretti, S.M.; Powers, S.J.; Pickett, J.A.; Birkett, M.A. The natural plant stress elicitor cis-jasmone causes cultivar-dependent reduction in growth of the stink bug, Euschistus heros and associated changes in flavonoid concentrations in soybean, Glycine max. Phytochemistry 2016, 131, 84–91. [Google Scholar] [CrossRef]
  53. Schwenen, L.; Komoßa, D.; Barz, W. Metabolism and degradation of nicotinic acid in parsley (Petroselinum hortense) cell suspension cultures and seedlings. Z. Naturforschung C 1986, 41, 148–157. [Google Scholar] [CrossRef]
  54. Wang, H.; Xu, C.; Zhang, Y.; Yan, X.; Jin, X.; Yao, X.; Chen, P.; Zheng, B. PtKTI12 genes influence wobble uridine modifications and drought stress tolerance in hybrid poplar. Tree Physiol. 2020, 40, 1778–1791. [Google Scholar] [CrossRef]
  55. Kuznetsov, V.; Shorina, M.; Aronova, E.; Stetsenko, L.; Rakitin, V.; Shevyakova, N. NaCl-and ethylene-dependent cadaverine accumulation and its possible protective role in the adaptation of the common ice plant to salt stress. Plant Sci. 2007, 172, 363–370. [Google Scholar] [CrossRef]
  56. Sun, T.; Pei, T.; Yang, L.; Zhang, Z.; Li, M.; Liu, Y.; Ma, F.; Liu, C. Exogenous application of xanthine and uric acid and nucleobase-ascorbate transporter MdNAT7 expression regulate salinity tolerance in apple. BMC Plant Biol. 2021, 21, 52. [Google Scholar] [CrossRef] [PubMed]
  57. Tan, S.; Cao, J.; Xia, X.; Li, Z. Advances in 5-Aminolevulinic Acid Priming to Enhance Plant Tolerance to Abiotic Stress. Int. J. Mol. Sci. 2022, 23, 702. [Google Scholar] [CrossRef] [PubMed]
  58. Jo, J.; Lee, J.; Ahn, Y.; Hwang, Y.S.; Park, J.; Lee, J.; Choi, J. Metabolome and transcriptome analyses of plants grown in naturally attenuated soil after hydrogen fluoride exposure. J. Hazard. Mater. 2022, 437, 129323. [Google Scholar] [CrossRef] [PubMed]
  59. Mohammadi, S.; Lalehloo, B.S.; Bayat, M.; Sharafi, S.; Habibi, F. Using physiological traits to evaluating resistance of different barley promising lines to water deficit stress. Int. J. Sci. Res. Environ. Sci. 2014, 2, 209. [Google Scholar] [CrossRef]
  60. Benjamin, J.J.; Lucini, L.; Jothiramshekar, S.; Parida, A. Metabolomic insights into the mechanisms underlying tolerance to salinity in different halophytes. Plant Physiol. Biochem. 2019, 135, 528–545. [Google Scholar] [CrossRef]
  61. Chamoun, R.; Aliferis, K.A.; Jabaji, S. Identification of signatory secondary metabolites during mycoparasitism of Rhizoctonia solani by Stachybotrys elegans. Front. Microbiol. 2015, 6, 138210. [Google Scholar] [CrossRef]
  62. Moulin, M.; Deleu, C.; Larher, F.; Bouchereau, A. The lysine-ketoglutarate reductase–saccharopine dehydrogenase is involved in the osmo-induced synthesis of pipecolic acid in rapeseed leaf tissues. Plant Physiol. Biochem. 2006, 44, 474–482. [Google Scholar] [CrossRef]
  63. Mano, Y.; Nemoto, K. The pathway of auxin biosynthesis in plants. J. Exp. Bot. 2012, 63, 2853–2872. [Google Scholar] [CrossRef]
  64. Jiang, Z.; Zhang, H.; Jiao, P.; Wei, X.; Liu, S.; Guan, S.; Ma, Y. The Integration of Metabolomics and Transcriptomics Provides New Insights for the Identification of Genes Key to Auxin Synthesis at Different Growth Stages of Maize. Int. J. Mol. Sci. 2022, 23, 13195. [Google Scholar] [CrossRef]
  65. Bayoumi, S.A.; Rowan, M.G.; Beeching, J.R.; Blagbrough, I.S. Investigation of biosynthetic pathways to hydroxycoumarins during post-harvest physiological deterioration in cassava roots by using stable isotope labelling. ChemBioChem 2008, 9, 3013–3022. [Google Scholar] [CrossRef]
  66. Zhao, Y.; Wang, N.; Sui, Z.; Huang, C.; Zeng, Z.; Kong, L. The molecular and structural basis of O-methylation reaction in coumarin biosynthesis in Peucedanum praeruptorum Dunn. Int. J. Mol. Sci. 2019, 20, 1533. [Google Scholar] [CrossRef]
  67. Liu, X.; Zhang, S.; Whitworth, R.J.; Stuart, J.J.; Chen, M.-S. Unbalanced activation of glutathione metabolic pathways suggests potential involvement in plant defense against the gall midge Mayetiola destructor in wheat. Sci. Rep. 2015, 5, 8092. [Google Scholar] [CrossRef]
  68. Youssef, S.A.; Tartoura, K.A. Compost enhances plant resistance against the bacterial wilt pathogen Ralstonia solanacearum via up-regulation of ascorbate-glutathione redox cycle. Eur. J. Plant Pathol. 2013, 137, 821–834. [Google Scholar] [CrossRef]
  69. Schalk, M.; Cabello-Hurtado, F.; Pierrel, M.-A.s.; Atanassova, R.; Saindrenan, P.; Werck-Reichhart, D.l. Piperonylic acid, a selective, mechanism-based inactivator of the trans-cinnamate 4-hydroxylase: A new tool to control the flux of metabolites in the phenylpropanoid pathway. Plant Physiol. 1998, 118, 209–218. [Google Scholar] [CrossRef]
  70. da Costa, T.P.S.; Hall, C.J.; Panjikar, S.; Wyllie, J.A.; Christoff, R.M.; Bayat, S.; Hulett, M.D.; Abbott, B.M.; Gendall, A.R.; Perugini, M.A. Towards novel herbicide modes of action by inhibiting lysine biosynthesis in plants. Elife 2021, 10, e69444. [Google Scholar]
  71. Dzierzbicka, K. Synthesis of 2, 6-diaminopimelic acid (DAP) and its analogues. Pol. J. Chem. 2007, 81, 455–473. [Google Scholar] [CrossRef]
  72. Dempsey, D.M.A.; Klessig, D.F. SOS–too many signals for systemic acquired resistance? Trends Plant Sci. 2012, 17, 538–545. [Google Scholar] [CrossRef]
  73. Návarová, H.; Bernsdorff, F.; Döring, A.-C.; Zeier, J. Pipecolic acid, an endogenous mediator of defense amplification and priming, is a critical regulator of inducible plant immunity. Plant Cell 2012, 24, 5123–5141. [Google Scholar] [CrossRef]
  74. Vranova, V.; Lojkova, L.; Rejsek, K.; Formanek, P. Significance of the natural occurrence of L-versus D-pipecolic acid: A review. Chirality 2013, 25, 823–831. [Google Scholar] [CrossRef]
  75. Prabhu, B.R.; Mulchandani, N.B. Biosynthesis of piperlongumine. Phytochemistry 1985, 24, 2589–2591. [Google Scholar] [CrossRef]
  76. Vogel-Adghough, D.; Stahl, E.; Návarová, H.; Zeier, J. Pipecolic acid enhances resistance to bacterial infection and primes salicylic acid and nicotine accumulation in tobacco. Plant Signal. Behav. 2013, 8, e26366. [Google Scholar] [CrossRef]
  77. Liu, S.; Xie, L.; Su, J.; Tian, B.; Fang, A.; Yu, Y.; Bi, C.; Yang, Y. Integrated metabolo-transcriptomics reveals the defense response of homogentisic acid in wheat against Puccinia striiformis f. sp. tritici. J. Agric. Food Chem. 2022, 70, 3719–3729. [Google Scholar] [CrossRef]
  78. Arruda, P.; Barreto, P. Lysine catabolism through the saccharopine pathway: Enzymes and intermediates involved in plant responses to abiotic and biotic stress. Front. Plant Sci. 2020, 11, 535796. [Google Scholar] [CrossRef]
  79. Delaney, T.P.; Uknes, S.; Vernooij, B.; Friedrich, L.; Weymann, K.; Negrotto, D.; Gaffney, T.; Gut-Rella, M.; Kessmann, H.; Ward, E. A central role of salicylic acid in plant disease resistance. Science 1994, 266, 1247–1250. [Google Scholar] [CrossRef]
  80. Alazem, M.; Lin, N.S. Roles of plant hormones in the regulation of host–virus interactions. Mol. Plant Pathol. 2015, 16, 529–540. [Google Scholar] [CrossRef]
  81. Grant, J.J.; Chini, A.; Basu, D.; Loake, G.J. Targeted activation tagging of the Arabidopsis NBS-LRR gene, ADR1, conveys resistance to virulent pathogens. Mol. Plant-Microbe Interact. 2003, 16, 669–680. [Google Scholar] [CrossRef]
  82. Zhu, X.; Soliman, A.; Islam, M.R.; Adam, L.R.; Daayf, F. Verticillium dahliae’s isochorismatase hydrolase is a virulence factor that contributes to interference with potato’s salicylate and jasmonate defense signaling. Front. Plant Sci. 2017, 8, 399. [Google Scholar] [CrossRef]
  83. Rochon, A.; Boyle, P.; Wignes, T.; Fobert, P.R.; Després, C. The coactivator function of Arabidopsis NPR1 requires the core of its BTB/POZ domain and the oxidation of C-terminal cysteines. Plant Cell 2006, 18, 3670–3685. [Google Scholar] [CrossRef]
  84. Cameron, R.K.; Paiva, N.L.; Lamb, C.J.; Dixon, R.A. Accumulation of salicylic acid and PR-1 gene transcripts in relation to the systemic acquired resistance (SAR) response induced by Pseudomonas syringae pv. tomato in Arabidopsis. Physiol. Mol. Plant Pathol. 1999, 55, 121–130. [Google Scholar] [CrossRef]
  85. Lincoln, J.E.; Sanchez, J.P.; Zumstein, K.; Gilchrist, D.G. Plant and animal PR1 family members inhibit programmed cell death and suppress bacterial pathogens in plant tissues. Mol. Plant Pathol. 2018, 19, 2111–2123. [Google Scholar] [CrossRef]
  86. Jain, D.; Khurana, J.P. Role of pathogenesis-related (PR) proteins in plant defense mechanism. In Molecular Aspects of Plant-Pathogen Interaction; Springer: Singapore, 2018; pp. 265–281. [Google Scholar]
  87. Yi, S.Y.; Shirasu, K.; Moon, J.S.; Lee, S.-G.; Kwon, S.-Y. The activated SA and JA signaling pathways have an influence on flg22-triggered oxidative burst and callose deposition. PLoS ONE 2014, 9, e88951. [Google Scholar] [CrossRef]
  88. Neuenschwander, U.; Vernooij, B.; Friedrich, L.; Uknes, S.; Kessmann, H.; Ryals, J. Is hydrogen peroxide a second messenger of salicylic acid in systemic acquired resistance? Plant J. 1995, 8, 227–233. [Google Scholar] [CrossRef]
  89. Kovács, J.; Poór, P.; Szepesi, Á.; Tari, I. Salicylic acid induced cysteine protease activity during programmed cell death in tomato plants. Acta Biol. Hung. 2016, 67, 148–158. [Google Scholar] [CrossRef]
  90. Greenberg, J.T.; Guo, A.; Klessig, D.F.; Ausubel, F.M. Programmed cell death in plants: A pathogen-triggered response activated coordinately with multiple defense functions. Cell 1994, 77, 551–563. [Google Scholar] [CrossRef]
  91. Mittler, R.; Del Pozo, O.; Meisel, L.; Lam, E. Pathogen-induced programmed cell death in plants, a possible defense mechanism. Dev. Genet. 1997, 21, 279–289. [Google Scholar] [CrossRef]
  92. Garattini, E.; Mendel, R.; Romão, M.J.; Wright, R.; Terao, M. Mammalian molybdo-flavoenzymes, an expanding family of proteins: Structure, genetics, regulation, function and pathophysiology. Biochem. J. 2003, 372, 15–32. [Google Scholar] [CrossRef]
  93. Bellés, J.M.; Garro, R.; Fayos, J.; Navarro, P.; Primo, J.; Conejero, V. Gentisic acid as a pathogen-inducible signal, additional to salicylic acid for activation of plant defenses in tomato. Mol. Plant-Microbe Interact. 1999, 12, 227–235. [Google Scholar] [CrossRef]
  94. Campos, L.; Granell, P.; Tárraga, S.; López-Gresa, P.; Conejero, V.; Bellés, J.M.; Rodrigo, I.; Lisón, P. Salicylic acid and gentisic acid induce RNA silencing-related genes and plant resistance to RNA pathogens. Plant Physiol. Biochem. 2014, 77, 35–43. [Google Scholar] [CrossRef]
  95. Yalpani, N.; León, J.; Lawton, M.A.; Raskin, I. Pathway of salicylic acid biosynthesis in healthy and virus-inoculated tobacco. Plant Physiol. 1993, 103, 315–321. [Google Scholar] [CrossRef]
  96. Agerbirk, N.; Olsen, C.E. Glucosinolate hydrolysis products in the crucifer Barbarea vulgaris include a thiazolidine-2-one from a specific phenolic isomer as well as oxazolidine-2-thiones. Phytochemistry 2015, 115, 143–151. [Google Scholar] [CrossRef]
  97. Brader, G.; Mikkelsen, M.D.; Halkier, B.A.; Tapio Palva, E. Altering glucosinolate profiles modulates disease resistance in plants. Plant J. 2006, 46, 758–767. [Google Scholar] [CrossRef] [PubMed]
  98. Rodrigues, A.S.; Rosa, E.A.S. Effect of post-harvest treatments on the level of glucosinolates in broccoli. J. Sci. Food Agric. 1999, 79, 1028–1032. [Google Scholar] [CrossRef]
  99. Sanchez-Vallet, A.; Ramos, B.; Bednarek, P.; López, G.; Piślewska-Bednarek, M.; Schulze-Lefert, P.; Molina, A. Tryptophan-derived secondary metabolites in Arabidopsis thaliana confer non-host resistance to necrotrophic Plectosphaerella cucumerina fungi. Plant J. 2010, 63, 115–127. [Google Scholar] [CrossRef]
  100. Smolinska, U.; Knudsen, G.; Morra, M.; Borek, V. Inhibition of Aphanomyces euteiches f. sp. pisi by volatiles produced by hydrolysis of Brassica napus seed meal. Plant Dis. 1997, 81, 288–292. [Google Scholar] [CrossRef]
  101. Van Eylen, D.; Bellostas, N.; Strobel, B.W.; Oey, I.; Hendrickx, M.; Van Loey, A.; Sørensen, H.; Sørensen, J.C. Influence of pressure/temperature treatments on glucosinolate conversion in broccoli (Brassica oleraceae L. cv Italica) heads. Food Chem. 2009, 112, 646–653. [Google Scholar] [CrossRef]
  102. Fahey, J.W.; Zalcmann, A.T.; Talalay, P. The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 2001, 56, 5–51. [Google Scholar] [CrossRef]
  103. Singh, A.; Guest, D.; Copeland, L. Associations Between Glucosinolates, White Rust, and Plant Defense Activators in Brassica Plants: A Review. Int. J. Veg. Sci. 2014, 21, 297–313. [Google Scholar] [CrossRef]
  104. Zhai, K.; Liang, D.; Li, H.; Jiao, F.; Yan, B.; Liu, J.; Lei, Z.; Huang, L.; Gong, X.; Wang, X. NLRs guard metabolism to coordinate pattern-and effector-triggered immunity. Nature 2022, 601, 245–251. [Google Scholar] [CrossRef]
  105. Escaray, F.; Felipo-Benavent, A.; Vera, P. Linking plant metabolism and immunity through methionine biosynthesis. Mol. Plant 2022, 15, 6–8. [Google Scholar] [CrossRef] [PubMed]
  106. Yan, X.; Ma, L.; Pang, H.; Wang, P.; Liu, L.; Cheng, Y.; Cheng, J.; Guo, Y.; Li, Q. METHIONINE SYNTHASE1 is involved in chromatin silencing by maintaining DNA and histone methylation. Plant Physiol. 2019, 181, 249–261. [Google Scholar] [CrossRef]
  107. Byeon, Y.; Lee, H.J.; Lee, H.Y.; Back, K. Cloning and functional characterization of the Arabidopsis N-acetylserotonin O-methyltransferase responsible for melatonin synthesis. J. Pineal. Res. 2016, 60, 65–73. [Google Scholar] [CrossRef] [PubMed]
  108. Byeon, Y.; Choi, G.H.; Lee, H.Y.; Back, K. Melatonin biosynthesis requires N-acetylserotonin methyltransferase activity of caffeic acid O-methyltransferase in rice. J. Exp. Bot. 2015, 66, 6917–6925. [Google Scholar] [CrossRef] [PubMed]
  109. Wei, Y.; Liu, G.; Bai, Y.; Xia, F.; He, C.; Shi, H.; Foyer, C. Two transcriptional activators of N-acetylserotonin O-methyltransferase 2 and melatonin biosynthesis in cassava. J. Exp. Bot. 2017, 68, 4997–5006. [Google Scholar] [CrossRef] [PubMed]
  110. Lee, H.Y.; Byeon, Y.; Back, K. Melatonin as a signal molecule triggering defense responses against pathogen attack in Arabidopsis and tobacco. J. Pineal. Res. 2014, 57, 262–268. [Google Scholar] [CrossRef]
  111. Kong, M.; Sheng, T.; Liang, J.; Ali, Q.; Gu, Q.; Wu, H.; Chen, J.; Liu, J.; Gao, X. Melatonin and Its Homologs Induce Immune Responses via Receptors trP47363-trP13076 in Nicotiana benthamiana. Front. Plant Sci. 2021, 12, 691835. [Google Scholar] [CrossRef]
  112. Li, M.; Zhang, X.; Li, J.; Ali, M.; Wang, Y.; Liu, X.; Li, F.; Li, X. GABA primes defense responses against Botrytis cinerea in tomato fruit by modulating ethylene and JA signaling pathways. Postharvest Biol. Technol. 2024, 208, 112665. [Google Scholar] [CrossRef]
  113. Xuan Phong, H.; Le Viet, Q.; Minh Chau, L.; Long, D.; Bui, H.; Thanh, N.N.; Tan Phat, D.; Truong, L.D. Isolation and selection of lactic acid bacteria with the capacity of producing γ-aminobutyric acid (GABA) and antimicrobial activity: Its application in fermented meat product. Curr. Nutr. Food Sci. 2023, 19, 831–837. [Google Scholar] [CrossRef]
  114. Guo, Z.; Lv, J.; Dong, X.; Du, N.; Piao, F. Gamma-aminobutyric acid improves phenanthrene phytotoxicity tolerance in cucumber through the glutathione-dependent system of antioxidant defense. Ecotoxicol. Environ. Saf. 2021, 217, 112254. [Google Scholar] [CrossRef]
  115. Hijaz, F.; Nehela, Y.; Killiny, N. Application of gamma-aminobutyric acid increased the level of phytohormones in Citrus sinensis. Planta 2018, 248, 909–918. [Google Scholar] [CrossRef]
  116. Meher, H.C.; Gajbhiye, V.T.; Singh, G. Salicylic acid-induced glutathione status in tomato crop and resistance to root-knot nematode, Meloidogyne incognita (Kofoid & White) Chitwood. J. Xenobiotics 2011, 1, e5. [Google Scholar]
  117. Meher, H.C.; Gajbhiye, V.T.; Singh, G.; Chawla, G. Altered metabolomic profile of selected metabolites and improved resistance of Cicer arietinum (L.) against Meloidogyne incognita (Kofoid & White) Chitwood following seed soaking with salicylic acid, benzothiadiazole or nicotinic acid. Acta Physiol. Plant. 2015, 37, 1–12. [Google Scholar]
  118. Hiruma, K.; Fukunaga, S.; Bednarek, P.; Piślewska-Bednarek, M.; Watanabe, S.; Narusaka, Y.; Shirasu, K.; Takano, Y. Glutathione and tryptophan metabolism are required for Arabidopsis immunity during the hypersensitive response to hemibiotrophs. Proc. Natl. Acad. Sci. USA 2013, 110, 9589–9594. [Google Scholar] [CrossRef] [PubMed]
  119. Chen, Y.-P.; Xing, L.-P.; Wu, G.-J.; Wang, H.-Z.; Wang, X.-E.; Cao, A.-Z.; Chen, P.-D. Plastidial glutathione reductase from Haynaldia villosa is an enhancer of powdery mildew resistance in wheat (Triticum aestivum). Plant Cell Physiol. 2007, 48, 1702–1712. [Google Scholar] [CrossRef] [PubMed]
  120. Sova, M. Antioxidant and antimicrobial activities of cinnamic acid derivatives. Mini Rev. Med. Chem. 2012, 12, 749–767. [Google Scholar] [CrossRef]
  121. Muroi, A.; Ishihara, A.; Tanaka, C.; Ishizuka, A.; Takabayashi, J.; Miyoshi, H.; Nishioka, T. Accumulation of hydroxycinnamic acid amides induced by pathogen infection and identification of agmatine coumaroyltransferase in Arabidopsis thaliana. Planta 2009, 230, 517–527. [Google Scholar] [CrossRef]
  122. Guo, M.; Li, C.; Huang, R.; Qu, L.; Liu, J.; Zhang, C.; Ge, Y. Ferulic acid enhanced resistance against blue mold of Malus domestica by regulating reactive oxygen species and phenylpropanoid metabolism. Postharvest Biol. Technol. 2023, 202, 112378. [Google Scholar] [CrossRef]
  123. Gozzo, F. Systemic acquired resistance in crop protection: From nature to a chemical approach. J. Agric. Food Chem. 2003, 51, 4487–4503. [Google Scholar] [CrossRef]
  124. Zhao, X.; Li, P.; Liu, X.; Xu, T.; Zhang, Y.; Meng, H.; Xia, T. High temperature increased lignin contents of poplar (Populus spp.) stem via inducing the synthesis caffeate and coniferaldehyde. Front. Genet. 2022, 13, 1007513. [Google Scholar] [CrossRef]
  125. Tang, Y.; Zhang, Z.; Lei, Y.; Hu, G.; Liu, J.; Hao, M.; Chen, A.; Peng, Q.; Wu, J. Cotton WATs modulate SA biosynthesis and local lignin deposition participating in plant resistance against Verticillium dahliae. Front. Plant Sci. 2019, 10, 526. [Google Scholar] [CrossRef]
  126. Wang, J.-Z.; Yan, C.-H.; Zhang, X.-R.; Tu, Q.-B.; Xu, Y.; Sheng, S.; Wu, F.-A.; Wang, J. A novel nanoparticle loaded with methyl caffeate and caffeic acid phenethyl ester against Ralstonia solanacearum—A plant pathogenic bacteria. RSC Adv. 2020, 10, 3978–3990. [Google Scholar] [CrossRef]
  127. Vogt, T. Phenylpropanoid biosynthesis. Mol. Plant 2010, 3, 2–20. [Google Scholar] [CrossRef] [PubMed]
  128. McCalla, D.; Neish, A. Metabolism of phenylpropanoid compounds in Salvia: II. Biosynthesis of phenolic cinnamic acids. Can. J. Biochem. Physiol. 1959, 37, 537–547. [Google Scholar] [CrossRef] [PubMed]
  129. Dixon, R.A.; Achnine, L.; Kota, P.; Liu, C.J.; Reddy, M.S.; Wang, L. The phenylpropanoid pathway and plant defence—A genomics perspective. Mol. Plant Pathol. 2002, 3, 371–390. [Google Scholar] [CrossRef]
  130. Wiklund, P.; Bergman, J. The chemistry of anthranilic acid. Curr. Org. Synth. 2006, 3, 379–402. [Google Scholar] [CrossRef]
  131. Winter, A. A hypothetical route for the biogenisis of IAA. Planta 1966, 71, 229–239. [Google Scholar] [CrossRef]
  132. Doyle, S.M.; Rigal, A.; Grones, P.; Karady, M.; Barange, D.K.; Majda, M.; Parizkova, B.; Karampelias, M.; Zwiewka, M.; Pencik, A.; et al. A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis. New Phytol. 2019, 223, 1420–1432. [Google Scholar] [CrossRef]
  133. Yang, S.Y.; Park, M.R.; Kim, I.S.; Kim, Y.C.; Yang, J.W.; Ryu, C.-M. 2-Aminobenzoic acid of Bacillus sp. BS107 as an ISR determinant against Pectobacterium carotovorum subsp. carotovotrum SCC1 in tobacco. Eur. J. Plant Pathol. 2011, 129, 371–378. [Google Scholar] [CrossRef]
  134. Hossain, M.; Hossain, M.; Islam, R.; Alam, A.; Zahan, K.; Sarkar, S.; Farooque, M. Antimicrobial and cytotoxic activities of 2-aminobenzoic acid and 2-aminophenol and their coordination complexes with Magnesium (Mg-II). Pak. J. Biol. Sci. 2004, 7, 25–27. [Google Scholar] [CrossRef]
  135. Zhang, Z.; Bi, X.; Du, X.; Liu, H.; An, T.; Zhao, Y.; Yu, H.; Chen, Y.; Wen, J. Comparative metabolomics reveal the participation of soybean unique rhizosphere metabolites in susceptibility and resistance of host soybean to Phytophthora sojae. Plant Soil 2022, 480, 185–199. [Google Scholar] [CrossRef]
  136. Iriti, M.; Rossoni, M.; Borgo, M.; Faoro, F. Benzothiadiazole enhances resveratrol and anthocyanin biosynthesis in grapevine, meanwhile improving resistance to Botrytis cinerea. J. Agric. Food Chem. 2004, 52, 4406–4413. [Google Scholar] [CrossRef]
  137. Takahashi, Y. The role of polyamines in plant disease resistance. Environ. Control Biol. 2016, 54, 17–21. [Google Scholar] [CrossRef]
  138. Kusano, T.; Yamaguchi, K.; Berberich, T.; Takahashi, Y. Advances in polyamine research in 2007. J. Plant Res. 2007, 120, 345–350. [Google Scholar] [CrossRef] [PubMed]
  139. Takahashi, Y.; Berberich, T.; Yamashita, K.; Uehara, Y.; Miyazaki, A.; Kusano, T. Identification of tobacco HIN1 and two closely related genes as spermine-responsive genes and their differential expression during the Tobacco mosaic virus-induced hypersensitive response and during leaf-and flower-senescence. Plant Mol. Biol. 2004, 54, 613–622. [Google Scholar] [CrossRef]
  140. Yamakawa, H.; Kamada, H.; Satoh, M.; Ohashi, Y. Spermine is a salicylate-independent endogenous inducer for both tobacco acidic pathogenesis-related proteins and resistance against tobacco mosaic virus infection. Plant Physiol. 1998, 118, 1213–1222. [Google Scholar] [CrossRef]
  141. Zhao, Y.; Han, G.; Li, Y.; Lv, H. Changes in quality characteristics and metabolites composition of wheat under different storage temperatures. J. Stored Prod. Res. 2024, 105, 102229. [Google Scholar] [CrossRef]
  142. Li, X.; Zhang, J.; Lin, S.; Xing, Y.; Zhang, X.; Ye, M.; Chang, Y.; Guo, H.; Sun, X. (+)-Catechin, epicatechin and epigallocatechin gallate are important inducible defensive compounds against Ectropis grisescens in tea plants. Plant Cell Environ. 2022, 45, 496–511. [Google Scholar] [CrossRef]
  143. Piispanen, J.; Bergmann, U.; Karhu, J.; Kauppila, T.; Kaitera, J. Variation of compounds in leaves of susceptible and resistant alternate hosts of Cronartium pini and C. ribicola. Eur. J. Plant Pathol. 2023, 165, 677–692. [Google Scholar] [CrossRef]
  144. Wu, H.; Wu, L.; Wang, J.; Zhu, Q.; Lin, S.; Xu, J.; Zheng, C.; Chen, J.; Qin, X.; Fang, C. Mixed phenolic acids mediated proliferation of pathogens Talaromyces helicus and Kosakonia sacchari in continuously monocultured Radix pseudostellariae rhizosphere soil. Front. Microbiol. 2016, 7, 335. [Google Scholar] [CrossRef]
  145. Schoch, G.A.; Nikov, G.N.; Alworth, W.L.; Werck-Reichhart, D. Chemical inactivation of the cinnamate 4-hydroxylase allows for the accumulation of salicylic acid in elicited cells. Plant Physiol. 2002, 130, 1022–1031. [Google Scholar] [CrossRef]
  146. Desmedt, W.; Jonckheere, W.; Nguyen, V.H.; Ameye, M.; De Zutter, N.; De Kock, K.; Debode, J.; Van Leeuwen, T.; Audenaert, K.; Vanholme, B. The phenylpropanoid pathway inhibitor piperonylic acid induces broad-spectrum pest and disease resistance in plants. Plant Cell Environ. 2021, 44, 3122–3139. [Google Scholar] [CrossRef]
  147. Nawrath, C.; Métraux, J.-P. Salicylic acid induction–deficient mutants of Arabidopsis express PR-2 and PR-5 and accumulate high levels of camalexin after pathogen inoculation. Plant Cell 1999, 11, 1393–1404. [Google Scholar] [PubMed]
  148. Larkan, N.J.; Yu, F.; Lydiate, D.J.; Rimmer, S.R.; Borhan, M.H. Single R gene introgression lines for accurate dissection of the Brassica-Leptosphaeria pathosystem. Front. Plant Sci. 2016, 7, 1771. [Google Scholar] [CrossRef] [PubMed]
  149. Fu, F.; Liu, X.; Wang, R.; Zhai, C.; Peng, G.; Yu, F.; Fernando, W.D. Fine mapping of Brassica napus blackleg resistance gene Rlm1 through bulked segregant RNA sequencing. Sci. Rep. 2019, 9, 14600. [Google Scholar] [CrossRef] [PubMed]
  150. Sharpe, A.; Parkin, I.; Keith, D.; Lydiate, D. Frequent nonreciprocal translocations in the amphidiploid genome of oilseed rape (Brassica napus). Genome 1995, 38, 1112–1121. [Google Scholar] [CrossRef]
  151. Yu, F.; Lydiate, D.J.; Gugel, R.; Sharpe, A.; Rimmer, S. Introgression of Brassica rapa subsp. sylvestris blackleg resistance into B. napus. Mol. Breed. 2012, 30, 1495–1506. [Google Scholar] [CrossRef]
  152. Chen, Y.; Fernando, W. Prevalence of pathogenicity groups of Leptosphaeria maculans in western Canada and North Dakota, USA. Can. J. Plant Pathol. 2006, 28, 533–539. [Google Scholar] [CrossRef]
  153. Koch, E.; Badawy, H.; Hoppe, H. Differences between aggressive and non-aggressive single spore lines of Leptosphaeria maculans in cultural characteristics and phytotoxin production. J. Phytopathol. 1989, 124, 52–62. [Google Scholar] [CrossRef]
  154. Li, H.; Sivasithamparam, K.; Barbetti, M.J.; Kuo, J. Germination and invasion by ascospores and pycnidiospores of Leptosphaeria maculans on spring-type Brassica napus canola varieties with varying susceptibility to blackleg. J. Gen. Plant Pathol. 2004, 70, 261–269. [Google Scholar] [CrossRef]
  155. Zhao, S.; Luo, X.; Li, L. Chemical isotope labeling LC-MS for high coverage and quantitative profiling of the hydroxyl submetabolome in metabolomics. Anal. Chem. 2016, 88, 10617–10623. [Google Scholar] [CrossRef]
  156. Chambers, M.C.; Maclean, B.; Burke, R.; Amodei, D.; Ruderman, D.L.; Neumann, S.; Gatto, L.; Fischer, B.; Pratt, B.; Egertson, J. A cross-platform toolkit for mass spectrometry and proteomics. Nat. Biotechnol. 2012, 30, 918–920. [Google Scholar] [CrossRef]
  157. Zhou, R.; Tseng, C.-L.; Huan, T.; Li, L. IsoMS: Automated processing of LC-MS data generated by a chemical isotope labeling metabolomics platform. Anal. Chem. 2014, 86, 4675–4679. [Google Scholar] [CrossRef] [PubMed]
  158. Wu, Y.; Li, L. Sample normalization methods in quantitative metabolomics. J. Chromatogr. A 2016, 1430, 80–95. [Google Scholar] [CrossRef] [PubMed]
  159. R Core Team. R: A Language and Environment for Statistical Computing; Foundation for Statistical Computing: Vienna, Austria, 2024. [Google Scholar]
  160. Posit Team. RStudio: Integrated Development Environment for R; Posit Software, PBC: Boston, MA, USA, 2024. [Google Scholar]
  161. Love, M.I.; Huber, W.; Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014, 15, 1–21. [Google Scholar] [CrossRef]
  162. Wickham, H. ggplot2. Wiley Interdiscip. Rev. Comput. Stat. 2011, 3, 180–185. [Google Scholar] [CrossRef]
  163. Wickham, H. ggplot2: Elegant Graphics for Data Analysis; Springer: New York, NY, USA, 2016. [Google Scholar]
  164. Wickham, H.; Chang, W.; Henry, L.; Pedersen, T.L.; Takahashi, K.; Wilke, C.; Woo, K.; Yutani, H.; Dunnington, D.; van den Brand, T. Create Elegant Data Visualisations Using the Grammar of Graphics. Version 3.5.1. CRAN. 2024. Available online: https://ggplot2.tidyverse.org (accessed on 30 May 2025).
  165. Yan, L. ggvenn: Draw Venn Diagram by ‘ggplot2’. R Package Version 0.1.10. CRAN. 2023. Available online: https://CRAN.R-project.org/package=ggvenn (accessed on 30 May 2025).
  166. Kolde, R. Pretty Heatmaps. R Package Version 1.0.12. CRAN. 2019. Available online: https://CRAN.R-project.org/package=pheatmap (accessed on 30 May 2025).
  167. Kassambara, A. rstatix: Pipe-Friendly Framework for Basic Statistical Tests. R Package Version 0.7.2. CRAN. 2023. Available online: https://CRAN.R-project.org/package=rstatix (accessed on 30 May 2025).
  168. de Mendiburu, F. Agricolae: Statistical Procedures for Agricultural Research. R Package Version 1.3-7. CRAN. 2023. Available online: https://CRAN.R-project.org/package=agricolae (accessed on 30 May 2025).
  169. Wobbrock, J.O.; Findlater, L.; Gergle, D.; Higgins, J.J. The aligned rank transform for nonparametric factorial analyses using only anova procedures. In Proceedings of the ACM Conference on Human Factors in Computing Systems (CHI 2011), Vancouver, BC, Canada, 7–12 May 2011; ACM Press: New York, NY, USA, 2011; pp. 143–146. [Google Scholar]
  170. Mangiafico, S.S. Aligned Ranks Transformation ANOVA. Summary and Analysis of Extension Program Evaluation in R; Rutgers Cooperative Extension: New Brunswick, NJ, USA, 2016; pp. 315–327. [Google Scholar]
  171. Elkin, L.A.; Kay, M.; Higgins, J.J.; Wobbrock, J.O. An Aligned Rank Transform Procedure for Multifactor Contrast Tests. In Proceedings for The 34th annual ACM Symposium on User Interface Software and Technology; Nichols, J., Kumar, R., Eds.; Association for Computing Machinery: New York, NY, USA, 2021; pp. 754–768. [Google Scholar]
  172. Kay, M.; Elkin, L.A.; Higgins, J.J.; Wobbrock, J.O. ARTool: Aligned Rank Transform. Version 0.10.7. CRAN. 2020. Available online: https://CRAN.R-project.org/package=ARTool (accessed on 30 May 2025).
  173. Lenth, R.V. emmeans: Estimated Marginal Means, Aka Least-Squares Means. R Package Version 1.10.4. CRAN. 2024. Available online: https://CRAN.R-project.org/package=emmeans (accessed on 30 May 2025).
  174. Mangiafico, S. rcompanion: Functions to Support Extension Education Program Evaluation; Version 2.4.36; CRAN; Rutgers Cooperative Extension: New Brunswick, NJ, USA, 2024; Available online: https://CRAN.R-project.org/package=rcompanion (accessed on 30 May 2025).
  175. Yurekten, O.; Payne, T.; Tejera, N.; Amaladoss, F.X.; Martin, C.; Williams, M.; O’Donovan, C. MetaboLights: Open data repository for metabolomics. Nucleic Acids Res. 2024, 52, D640–D646. [Google Scholar] [CrossRef]
Figure 1. The number of differentially up- (A,C) and down- (B,D) accumulated metabolites (DAMs) in inoculated (I) and control (C) samples of Topas (T) and Topas–Rlm1 (R) within the same treatment collected at 3, 7, and 11 days post-inoculation (dpi). RIvsRC_3D represents the number of DAMs between inoculated and control Topas–Rlm1 samples collected at 3 dpi. Similarly, TIvsTC_11D shows inoculated and control Topas collected at 11 dpi, and so on.
Figure 1. The number of differentially up- (A,C) and down- (B,D) accumulated metabolites (DAMs) in inoculated (I) and control (C) samples of Topas (T) and Topas–Rlm1 (R) within the same treatment collected at 3, 7, and 11 days post-inoculation (dpi). RIvsRC_3D represents the number of DAMs between inoculated and control Topas–Rlm1 samples collected at 3 dpi. Similarly, TIvsTC_11D shows inoculated and control Topas collected at 11 dpi, and so on.
Ijms 26 05627 g001
Figure 2. The number of significantly up- (A,C,E) and down-regulated (B,D,F) DAMs among samples between different treatments collected at 3, 7, and 11 dpi.
Figure 2. The number of significantly up- (A,C,E) and down-regulated (B,D,F) DAMs among samples between different treatments collected at 3, 7, and 11 dpi.
Ijms 26 05627 g002
Figure 3. Heatmap clustering analysis of Topas–Rlm1 and Topas at different stages of infection over (A) all metabolites and (B) differentially accumulated metabolites (DAMs). Red and blue colors in scale bars represent up- and down-regulated metabolomes, respectively. Red arrows point to the clusters with significantly higher or suppressed accumulation in later stages of infection (7 and/or 11 dpi). Treatment names are consistent with those in Figure 1.
Figure 3. Heatmap clustering analysis of Topas–Rlm1 and Topas at different stages of infection over (A) all metabolites and (B) differentially accumulated metabolites (DAMs). Red and blue colors in scale bars represent up- and down-regulated metabolomes, respectively. Red arrows point to the clusters with significantly higher or suppressed accumulation in later stages of infection (7 and/or 11 dpi). Treatment names are consistent with those in Figure 1.
Ijms 26 05627 g003
Figure 4. Differentially accumulated metabolites associated with Rlm1-mediated resistance that are involved in: (A) degradation of lysine (Lys), (BF) biosynthesis of Lys, anthocyanin (ACN), glucosinolate (GSLs), scopoletin/isoscp (Scop/IsoScop), and melatonin (Mel), (G,H) metabolism of nicotinate-nicotinamide (NaN) and γ-aminobutyric acid (GABA), (I,J) defense-related isoflavonoids (Isoflav) and hormones, (K) and antimicrobial/plant-defense responses [45,46,47,48,49,50,51,52]. (L) Biosynthesis of tropane (Trop), piperidine (Pid), and pyridine alkaloid. (M) Biosynthesis of phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp). (N,O) Metabolism of Trp, taurine (Tau)/hypotaurine (HTau). (P) Other amino acids and their derivatives. (Q) Metabolism of Phe. (R) The glutamate family. (S) Response to abiotic stress [53,54,55,56,57,58,59,60]. Red and blue colors represent up- and down-regulated DAMs, respectively, while gray color indicates no changes from controls. Treatment names are consistent with those designated in Figure 1.
Figure 4. Differentially accumulated metabolites associated with Rlm1-mediated resistance that are involved in: (A) degradation of lysine (Lys), (BF) biosynthesis of Lys, anthocyanin (ACN), glucosinolate (GSLs), scopoletin/isoscp (Scop/IsoScop), and melatonin (Mel), (G,H) metabolism of nicotinate-nicotinamide (NaN) and γ-aminobutyric acid (GABA), (I,J) defense-related isoflavonoids (Isoflav) and hormones, (K) and antimicrobial/plant-defense responses [45,46,47,48,49,50,51,52]. (L) Biosynthesis of tropane (Trop), piperidine (Pid), and pyridine alkaloid. (M) Biosynthesis of phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp). (N,O) Metabolism of Trp, taurine (Tau)/hypotaurine (HTau). (P) Other amino acids and their derivatives. (Q) Metabolism of Phe. (R) The glutamate family. (S) Response to abiotic stress [53,54,55,56,57,58,59,60]. Red and blue colors represent up- and down-regulated DAMs, respectively, while gray color indicates no changes from controls. Treatment names are consistent with those designated in Figure 1.
Ijms 26 05627 g004aIjms 26 05627 g004bIjms 26 05627 g004c
Figure 5. Relative intensities of glutathione (GSH) and oxidized glutathione (GSSG) in LC–MS analysis, as well as their ratios, in Topas (T) and Topas–Rlm1 (R) canola receiving L. maculans (I) or water (C). (A,B) LC–MS intensity for GSH and GSSG. (C) The ratio of GSH/GSSG for each treatment. Data points with the same letter(s) across DAI (day after inoculation) did not differ significantly (p > 0.05, LSD).
Figure 5. Relative intensities of glutathione (GSH) and oxidized glutathione (GSSG) in LC–MS analysis, as well as their ratios, in Topas (T) and Topas–Rlm1 (R) canola receiving L. maculans (I) or water (C). (A,B) LC–MS intensity for GSH and GSSG. (C) The ratio of GSH/GSSG for each treatment. Data points with the same letter(s) across DAI (day after inoculation) did not differ significantly (p > 0.05, LSD).
Ijms 26 05627 g005
Figure 6. Suppression of L. maculans infection on cotyledons of Topas (moderately susceptible) and Westar (highly susceptible) following treatment with various metabolites. Treatments included pipecolic acid (PA, 40 mM), ferulic acid (FA, 1 mM), caffeic acid (CFA, 10 mM), benzoic acid (BA, 10 mM), salicylic acid (SA, 1 mM), gentisic acid (GA, 10 mM), 2,6-diaminopimelic acid (DAP, 30 mM), and piperonylic acid (PipA, 3 mM) applied prior to inoculation. Infection severity was assessed at 14 days post-inoculation. All treatments reduced infection compared to the control on both canola varieties (p < 0.05, LSD), except glutathione (GSH, 20 mM) and lysine (Lys, 10 mM), which showed efficacy only on Topas. Three inoculated cotyledons were photographed for each treatment to illustrate the range of symptoms observed.
Figure 6. Suppression of L. maculans infection on cotyledons of Topas (moderately susceptible) and Westar (highly susceptible) following treatment with various metabolites. Treatments included pipecolic acid (PA, 40 mM), ferulic acid (FA, 1 mM), caffeic acid (CFA, 10 mM), benzoic acid (BA, 10 mM), salicylic acid (SA, 1 mM), gentisic acid (GA, 10 mM), 2,6-diaminopimelic acid (DAP, 30 mM), and piperonylic acid (PipA, 3 mM) applied prior to inoculation. Infection severity was assessed at 14 days post-inoculation. All treatments reduced infection compared to the control on both canola varieties (p < 0.05, LSD), except glutathione (GSH, 20 mM) and lysine (Lys, 10 mM), which showed efficacy only on Topas. Three inoculated cotyledons were photographed for each treatment to illustrate the range of symptoms observed.
Ijms 26 05627 g006
Figure 7. Effect of post-inoculation treatments with pipecolic acid (PA, 40 mM) on infection of Topas and Westar cotyledons inoculated with L. maculans. (A) Symptoms at 14 dai (days after inoculation). Three inoculated cotyledons were photographed for each treatment to show the range of symptoms observed. (B) The mean infection severity where treatments with different letters are significantly different (p < 0.05, LSD).
Figure 7. Effect of post-inoculation treatments with pipecolic acid (PA, 40 mM) on infection of Topas and Westar cotyledons inoculated with L. maculans. (A) Symptoms at 14 dai (days after inoculation). Three inoculated cotyledons were photographed for each treatment to show the range of symptoms observed. (B) The mean infection severity where treatments with different letters are significantly different (p < 0.05, LSD).
Ijms 26 05627 g007
Table 1. Pathways involved in Rlm1-mediated resistance at 3 dpi 1.
Table 1. Pathways involved in Rlm1-mediated resistance at 3 dpi 1.
PathwaysTotal CompoundsHitsRaw pImpact
Flavone and flavonol biosynthesis1045.2649 × 10−50.5
Isoquinoline alkaloid biosynthesis610.0136140.5
Arginine and proline metabolism3230.119380.32738
Biosynthesis of various plant secondary metabolites2910.244850.24
Glycine, serine and threonine metabolism3320.0183560.22375
Lysine biosynthesis910.0001030.16216
Arginine biosynthesis1810.176050.13981
Tryptophan metabolism2910.0101770.10687
Glyoxylate and dicarboxylate metabolism2910.119360.10147
Tyrosine metabolism1710.0136140.10056
Phenylpropanoid biosynthesis4320.001280.09634
Glutathione metabolism2610.119360.07114
Pyrimidine metabolism4110.0210070.02929
Purine metabolism7330.0003160.02344
Phenylalanine, tyrosine, and tryptophan biosynthesis2220.0110810.02002
Flavonoid biosynthesis4724.2424 × 10−50.00338
Cysteine and methionine metabolism4710.0005620.00265
Lipoic acid metabolism2410.119360.0016
D-Amino acid metabolism710.0001030
Indole alkaloid biosynthesis410.0101770
Glucosinolate biosynthesis6510.0101770
Ubiquinone and other terpenoid-quinone biosynthesis4710.0136140
Anthocyanin biosynthesis1110.0145620
Lysine degradation2010.0388320
Thiamine metabolism2210.119360
Cyanoamino acid metabolism2920.167480
1 Pathway enrichment analysis was conducted using the MetaboAnalyst6.0 (https://www.metaboanalyst.ca; accessed on 10 October 2024) on DAMs identified in RI at 3 dpi [44]. ‘Total compounds’ refers to all potential metabolites associated with the pathway based on the MetaboAnalyst6.0 database. ‘Hits’ indicates the number of DAMs detected in the sample for the pathway. ‘Raw p’ represents unadjusted p-values from the pathway enrichment analysis. ‘Impact’ denotes the influence of DAMs on the pathway based on the enrichment analysis.
Table 2. Pathways involved in Rlm1-mediated resistance at 7 dpi 1.
Table 2. Pathways involved in Rlm1-mediated resistance at 7 dpi 1.
PathwaysTotal CompoundsHitsRaw pImpact
Taurine and hypotaurine metabolism520.0040921
Glutathione metabolism2650.0008430.51637
Isoquinoline alkaloid biosynthesis630.0002070.5
Tyrosine metabolism1740.0013640.32961
Glycine, serine and threonine metabolism3317.41 × 10−50.22375
Arginine biosynthesis1820.0202490.17088
Lysine degradation2035.38 × 10−50.16667
Lysine biosynthesis910.0008180.16216
Flavone and flavonol biosynthesis1020.000290.15
Arginine and proline metabolism3230.0401590.14584
Butanoate metabolism1710.136210.13636
Cysteine and methionine metabolism4720.0003110.13181
Alanine, aspartate, and glutamate metabolism2210.136210.1295
Purine metabolism7330.0010220.10374
Glyoxylate and dicarboxylate metabolism2917.41 × 10−50.10147
Phenylpropanoid biosynthesis4310.0178940.05935
Pyrimidine metabolism4110.0009130.02929
Folate biosynthesis3110.390260.02624
Porphyrin metabolism4810.0011980.02261
Ubiquinone and other terpenoid-quinone biosynthesis4724.84 × 10−50.02209
Phenylalanine, tyrosine, and tryptophan biosynthesis2224.84 × 10−50.02002
Tryptophan metabolism2930.0007220.01527
Lipoic acid metabolism2417.41 × 10−50.0016
Thiamine metabolism2217.41 × 10−50
Cyanoamino acid metabolism2928.89 × 10−50
Glucosinolate biosynthesis6530.0001280
Flavonoid biosynthesis4710.0001930
Biosynthesis of various plant secondary metabolites2910.0002480
Valine, leucine, and isoleucine degradation3710.0006620
Valine, leucine, and isoleucine biosynthesis2210.0006620
D-Amino acid metabolism710.0008180
Tropane, piperidine, and pyridine alkaloid biosynthesis920.0034530
Anthocyanin biosynthesis1110.0121090
Zeatin biosynthesis2110.0449670
1 Pathway enrichment analysis was conducted using the MetaboAnalyst6.0 (https://www.metaboanalyst.ca; accessed on 10 October 2024) on DAMs identified in RI at 7 dpi [44]. ‘Total compounds’ refers to all potential metabolites associated with the pathway based on the MetaboAnalyst6.0 database. ‘Hits’ indicates the number of DAMs detected in the sample for the pathway. ‘Raw p’ represents unadjusted p-values from the pathway enrichment analysis. ‘Impact’ denotes the influence of DAMs on the pathway based on the enrichment analysis.
Table 3. Pathways involved in Rlm1-mediated resistance at 11 dpi 1.
Table 3. Pathways involved in Rlm1-mediated resistance at 11 dpi 1.
PathwaysTotal CompoundsHitsRaw pImpact
Taurine and hypotaurine metabolism530.0036451
Phenylalanine metabolism1210.0001130.42308
Glutathione metabolism2639.13 × 10−50.40276
Tyrosine metabolism1750.0001410.39665
Phenylpropanoid biosynthesis4389.23 × 10−50.28583
Ubiquinone and other terpenoid-quinone biosynthesis4720.0004430.1998
beta-Alanine metabolism1820.0225680.19444
Lysine degradation2030.0007430.16667
Lysine biosynthesis910.0001920.16216
Arginine and proline metabolism3233.65 × 10−60.15774
Butanoate metabolism1710.0002990.13636
Alanine, aspartate and glutamate metabolism2210.0002990.1295
Purine metabolism7330.0034730.09255
Phenylalanine, tyrosine and tryptophan biosynthesis2231.11 × 10−50.09159
Arginine biosynthesis1810.0047430.08641
Pyrimidine metabolism4120.0027910.07198
Flavonoid biosynthesis4750.003630.06956
Cysteine and methionine metabolism4750.0005750.05644
Glucosinolate biosynthesis6531.36 × 10−60.04236
Tryptophan metabolism2944.93 × 10−50.03054
Pantothenate and CoA biosynthesis2510.169680.02796
Porphyrin metabolism4810.0133720.02261
Flavone and flavonol biosynthesis1026.24 × 10−50
Cyanoamino acid metabolism2910.0001130
Tropane, piperidine and pyridine alkaloid biosynthesis930.0001610
D-Amino acid metabolism710.0001920
Anthocyanin biosynthesis1120.000270
Glycine, serine and threonine metabolism3310.0027170
Zeatin biosynthesis2110.0056460
Isoquinoline alkaloid biosynthesis620.0064860
1 Pathway enrichment analysis was conducted using the MetaboAnalyst6.0 (https://www.metaboanalyst.ca; accessed on 10 October 2024) on DAMs identified in RI at 11 dpi [44]. ‘Total compounds’ refers to all potential metabolites associated with the pathway based on the MetaboAnalyst6.0 database. ‘Hits’ indicates the number of DAMs detected in the sample for the pathway. ‘Raw p’ represents unadjusted p-values from the pathway enrichment analysis. ‘Impact’ denotes the influence of DAMs on the pathway based on the enrichment analysis.
Table 4. Most differentially accumulated metabolites/hormones used to validate their involvement in Rlm1-mediated resistance to L. maculans in canola 1.
Table 4. Most differentially accumulated metabolites/hormones used to validate their involvement in Rlm1-mediated resistance to L. maculans in canola 1.
Common NameAbbreviationChemical NameMol. FormulaConcentration 2Supplier
Pipecolic acidPAPiperidine-2-carboxylic acidC6H11NO240 mMTokyo Chemical Industry (TCI)
(Portland, OR, USA)
Salicylic acid (sodium salt)SASodium 2-hydroxybenzoateC7H5NaO31 mMThermo Fisher
(Ottawa, ON, Canada)
Gentisic acid (sodium salt hydrate)GA2,5-Dihydroxybenzoic acid sodium saltC7H5O4Na10 mMSigma Aldrich
(MilliporeSigma Canada Ltd., Oakville, ON, Canada)
GlutathioneGSHγ-L-glutamyl-L-cysteinylglycineC6H11NO220 mMThermo Fisher
LysineLys(S)-2,6-Diaminocaproic acidC6H14N2O210 mMSigma Aldrich
Diaminopimelic acidDAP2,6-Diaminopimelic acidC7H14N2O430 mMSigma Aldrich
Ferulic acid FATrans-ferulic acidC10H10O41 mM 3Sigma Aldrich
Caffeic acid CFA(E)-3-(3,4-dihydroxyphenyl) prop-2-enoic acidC9H8O410 mM 4Sigma Aldrich
Benzoic acidBABenzoic acidC7H6O210 mM 5Thermo Fisher
Piperonylic acidPipA1,3-benzodioxole-5-carboxylic acidC8H6O43 mM 6Sigma Aldrich
1 The chromatogram and mass-spectral information for these metabolites are shown in Supplemental Figures S4–S11, except for BA and PipA. The identification of BA is tentative, based solely on Tier-3 database annotation. PipA was selected for testing due to its known role as an inhibitor of the phenylpropanoid pathway [69], aiming to confirm the involvement of several DAMs from this pathway that were suppressed during the Rlm1-mediated incompatible interaction. 2 The maximum concentration without causing visible impact on canola seedlings during a series of pretests. These metabolites were dissolved in deionized water to achieve the designated concentrations unless stated otherwise. 3,6 Dissolved initially in DMSO, then diluted with water to achieve 1 mM and 3 mM final concentrations with 0.17% and 1.7% DMSO, respectively. 4,5 Dissolved initially in 95% ethanol, then diluted with water to achieve the 10 mM final concentration with 9.5% and 6.8% ethanol, respectively.
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Zhu, X.; Gao, P.; Zhao, S.; Luo, X.; Li, L.; Peng, G. Metabolomic Profiling Identifies Key Metabolites and Defense Pathways in Rlm1-Mediated Blackleg Resistance in Canola. Int. J. Mol. Sci. 2025, 26, 5627. https://doi.org/10.3390/ijms26125627

AMA Style

Zhu X, Gao P, Zhao S, Luo X, Li L, Peng G. Metabolomic Profiling Identifies Key Metabolites and Defense Pathways in Rlm1-Mediated Blackleg Resistance in Canola. International Journal of Molecular Sciences. 2025; 26(12):5627. https://doi.org/10.3390/ijms26125627

Chicago/Turabian Style

Zhu, Xiaohan, Peng Gao, Shuang Zhao, Xian Luo, Liang Li, and Gary Peng. 2025. "Metabolomic Profiling Identifies Key Metabolites and Defense Pathways in Rlm1-Mediated Blackleg Resistance in Canola" International Journal of Molecular Sciences 26, no. 12: 5627. https://doi.org/10.3390/ijms26125627

APA Style

Zhu, X., Gao, P., Zhao, S., Luo, X., Li, L., & Peng, G. (2025). Metabolomic Profiling Identifies Key Metabolites and Defense Pathways in Rlm1-Mediated Blackleg Resistance in Canola. International Journal of Molecular Sciences, 26(12), 5627. https://doi.org/10.3390/ijms26125627

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop