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Horticulturae
Special Issues
Genetic Breeding and Germplasm Resources of Fruit and Vegetable Crops
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Genetic Breeding and Germplasm Resources of Fruit and Vegetable Crops

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Vegetable Production Systems".

Deadline for manuscript submissions: 5 September 2026 | Viewed by 3271

Special Issue Editors

Dr. Yi Wang

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Guest Editor
Beijing Key Laboratory of Grape Science and Enology, and State Key Laboratory of Plant Diversity and Specialty Crops, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
Interests: genomics and genetics; bioinformatics; evolution; comparative genomics
Special Issues, Collections and Topics in MDPI journals
Dr. Meixia Wang

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Guest Editor
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China
Interests: CRISPR; vegetable breeding; disease resistance
Dr. Haiyang Chen

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Guest Editor
College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China
Interests: abiotic stress; gene function; Solanaceae breeding

Special Issue Information

Dear Colleagues,

Genetic breeding is the ultimate goal of horticultural research, and the germplasm resources of a variety of horticultural crops can contribute in various ways. However, environmental issues and the diverse needs of humanity are becoming increasingly pertinent challenges for breeders. How to breed high-resistance and high-quality varieties has become a priority of horticultural scholars.

The purpose of this Special Issue, titled “Genetic Breeding and Germplasm Resources of Fruit and Vegetable Crops”, is to present innovative studies, tools, approaches, and techniques that could contribute to genetic breeding, such as the deep mining of germplasm, gene function, breeding marker development, new breeding methods or technologies, and any other innovations that relate to genetic breeding or germplasm mining.

Dr. Yi Wang
Dr. Meixia Wang
Dr. Haiyang Chen
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Horticulturae is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • genetic breeding
  • germplasm
  • gene function
  • breeding marker and technologies
  • population genetics

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Published Papers (3 papers)

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Research

18 pages, 3915 KB  
Article
Rapid Development of Clubroot-Resistant Germplasm in Chinese Cabbage
by Yufan Ran, Bo Shi, Ruiyu Han, Xiuxiu Xu, Yun Dai, Ying Zhang, Shaoxing Wang, Shifan Zhang, Fei Li, Guoliang Li, Rifei Sun, Shujiang Zhang and Hui Zhang
Horticulturae 2026, 12(3), 395; https://doi.org/10.3390/horticulturae12030395 - 23 Mar 2026
Viewed by 561
Abstract
Disease resistance breeding is an important direction for the genetic improvement of Chinese cabbage. The traditional elite variety ‘Yutian Baojian’ Chinese cabbage is highly regarded for its tall cylindrical head with a pointed tip, tightly twisted wrapper leaves, and sweet taste. However, long-term [...] Read more.
Disease resistance breeding is an important direction for the genetic improvement of Chinese cabbage. The traditional elite variety ‘Yutian Baojian’ Chinese cabbage is highly regarded for its tall cylindrical head with a pointed tip, tightly twisted wrapper leaves, and sweet taste. However, long-term cultivation has led to a significant decline in its resistance to clubroot caused by Plasmodiophora brassicae. To restore clubroot resistance while maintaining its desirable horticultural traits, this study used the clubroot-susceptible ‘Yutian Baojian’ as the recurrent parent and the resistant donor ‘Shaocai’, which carries the CRd resistance gene, to develop backcross populations. Using marker-assisted selection (MAS), plants were comprehensively screened based on foreground selection with markers tightly linked to the CRd gene, background selection with 73 genome-wide polymorphic markers, and phenotypic evaluation of horticultural traits, including plant height, plant spread, head shape, and soluble solids content. In the BC1 population, three individuals showing high genetic similarity were selected. From the BC2 population, four elite individuals were obtained, exhibiting 99.32% genetic similarity, stable clubroot resistance, and typical horticultural characteristics. Furthermore, three homozygous resistant inbred lines (BC2S2) with the ‘Yutian Baojian’ phenotype were developed. These results enrich the clubroot-resistant germplasm resources of Chinese cabbage and provide an effective MAS-based strategy for the precise improvement and germplasm innovation of local cultivars. Full article
(This article belongs to the Special Issue Genetic Breeding and Germplasm Resources of Fruit and Vegetable Crops)
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18 pages, 7252 KB  
Article
Genome-Wide Analysis of LEA Gene Family in Rosa chinensis ‘Old Blush’ and Cold-Induced Expression Patterns in Two Species
by Longzhen Li, Huayang Li, Shiyi Wang, Haining Sun, Yaping Kou, Ruidong Jia, Xin Zhao, Linbo Xu, Junjie Duan, Hong Ge and Shuhua Yang
Horticulturae 2026, 12(2), 136; https://doi.org/10.3390/horticulturae12020136 - 25 Jan 2026
Viewed by 686
Abstract
Late embryogenesis abundant (LEA) proteins play an essential role in plant growth under various abiotic stresses. In this study, we identified 23 RcLEA genes in Rosa chinensis ‘Old Blush’ and subsequently grouped them into eight clades according to phylogenetic relationships and conserved domain [...] Read more.
Late embryogenesis abundant (LEA) proteins play an essential role in plant growth under various abiotic stresses. In this study, we identified 23 RcLEA genes in Rosa chinensis ‘Old Blush’ and subsequently grouped them into eight clades according to phylogenetic relationships and conserved domain features by bioinformatics methods. And conserved protein motifs and gene structure are also analyzed. The cis-regulatory elements of RcLEA promoter are enriched with cis-regulatory elements relevant to abiotic stress adaptation. Comparative transcriptomics between two species revealed tissue-specific and cold-induced expression differences, highlighting distinct functional roles of LEA genes in growth and abiotic stress tolerance between Rosa chinensis ‘Old Blush’ and Rosa beggeriana. Furthermore, Quantitative Real-Time PCR (qRT-PCR validation confirmed divergent cold-responsive expression profiles of LEA genes in R. chinensis ‘Old Blush’ compared with the highly cold-tolerant R. beggeriana in four LEA homologous genes. These findings indicated that LEA acts as a cold-response gene in roses and provide foundation to breed cold-tolerant varieties of roses. Full article
(This article belongs to the Special Issue Genetic Breeding and Germplasm Resources of Fruit and Vegetable Crops)
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16 pages, 3190 KB  
Article
GC-MS Non-Target Metabolomics-Based Analysis of the Volatile Aroma in Cerasus humilis After Grafting with Different Rootstocks
by Gaixia Qiao, Jun Xie, Chun’e Zhang, Yujuan Liu, Xiaojing Guo, Qiaoxia Jia, Caixia Zhang and Meilong Xu
Horticulturae 2025, 11(8), 972; https://doi.org/10.3390/horticulturae11080972 - 16 Aug 2025
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Abstract
C. humilis is a small shrub belonging to the Rosaceae family, and grafting is one of the main ways for propagation. However, the influence of different rootstocks on volatile aroma is still unclear. In this study, an untargeted metabolomics approach based on gas [...] Read more.
C. humilis is a small shrub belonging to the Rosaceae family, and grafting is one of the main ways for propagation. However, the influence of different rootstocks on volatile aroma is still unclear. In this study, an untargeted metabolomics approach based on gas chromatography–mass spectrometry (GC-MS) was utilized to analyze the volatile differential metabolites between the rootstock–scion combinations and self-rooted seedlings. Furthermore, metabolic pathway enrichment analysis was performed using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. In total, 191,162 and 150 volatile differential metabolites were identified in different rootstock–scion combinations. The rootstock–scion combinations of ZG/MYT and ZG/BT could improve the volatile aroma in the fruit of C. humilis and made significant contributions to the rose and fruity flavors. KEGG pathway analysis indicated that the differential metabolites were mainly enriched in the butanoate metabolism and glycolysis/gluconeogenesis pathways, showing an increasing trend. Prunus tomentosa and Amygdalus communis can serve as preferred rootstocks for enhancing the aroma quality of C. humilis fruits. These results provide new insight into rootstock-based propagation and breeding and also offer some guidance for graft-based fruit production. Full article
(This article belongs to the Special Issue Genetic Breeding and Germplasm Resources of Fruit and Vegetable Crops)
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