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State-of-the-Art Technology for Rapid Detection of Pesticide/Chemical Residues in Foods
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State-of-the-Art Technology for Rapid Detection of Pesticide/Chemical Residues in Foods

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Toxicology".

Deadline for manuscript submissions: 15 October 2025 | Viewed by 2819

Special Issue Editor

Dr. Xinghua Zhou

E-Mail Website
Guest Editor
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
Interests: pesticide residue; veterinary drug residue; chemical residues in food and agricultural products; monoclonal antibody; polyclonal antibody; ic-ELISA; colloidal gold; immunochromatographic strip; immunochemical detection

Special Issue Information

Dear Colleagues,

Due to the excessive use of chemical pollutants in food and agricultural products to meet the global demand for agricultural and animal products, pesticide and veterinary residues pose an increasing threat to ecosystems and human health. Some other environmental contaminants used in our daily commodities may cause health risks. Therefore, the demand for the routine detection of these chemical residues in food and agricultural products has increased quickly all over the world. Immunoassay is suitable for rapid detection of a large number of samples because of its high sensitivity, specificity, simplicity, rapidity, and low test cost. In this regard, many novel and effective methods have been developed and reported, including immunochemical detection, the enzyme inhibition method, fluorescent turn-on probes, nanomaterial biosensors, surface-enhanced Raman spectroscopy, etc. These methods or platforms have become powerful analytical methods for the rapid detection of pesticide residues or chemical pollutants due to their several advantages, including synergy, systematic manufacturing procedures, ease of detection, and significant sensitivity and selectivity. Furthermore, some modern methods, such as portable electrochemical devices and optical smartphone-based sensors, have been fabricated recently for the point-of-care and on-site rapid detection of residues of pesticides and other chemicals.

Dr. Xinghua Zhou
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • rapid detection technology
  • pesticide
  • veterinary drug
  • heavy metal
  • allergen
  • monoclonal antibody
  • polyclonal antibody
  • ic-ELISA (an indirect competitive enzyme-linked immunosorbent assay)
  • colloidal gold immunochromatographic strip
  • fluoroimmunoassay
  • nanomaterial biosensors
  • surface-enhanced Raman spectroscopy

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Published Papers (2 papers)

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Research

16 pages, 5476 KiB  
Article
Development of a Colloidal Gold Immunochromatographic Assay Strip Using a Monoclonal Antibody for the Rapid Detection of Ofloxacin
by Xiaolan Li, Jin Huang, Na Li, Mahmoud Salah, Shuoning Guan, Wenwen Pan, Ziyi Wang, Xinghua Zhou and Yun Wang
Foods 2024, 13(24), 4137; https://doi.org/10.3390/foods13244137 - 20 Dec 2024
Viewed by 842
Abstract
The livestock industry uses ofloxacin, an antibiotic, to prevent several animal diseases; however, the overdose of ofloxacin used in animal farming treatments may appear in food products and cause some adverse human health effects. Hence, there is an immediate need to develop a [...] Read more.
The livestock industry uses ofloxacin, an antibiotic, to prevent several animal diseases; however, the overdose of ofloxacin used in animal farming treatments may appear in food products and cause some adverse human health effects. Hence, there is an immediate need to develop a method suitable for on site large-scale detection of ofloxacin residues in animal-derived foods. This study aimed to prepare a monoclonal antibody with high sensitivity and affinity for ofloxacin by re-synthesizing the ofloxacin hapten and synthesizing the corresponding complete antigen. The IC50 of the enzyme-linked immunosorbent assay (ic-ELISA) was 0.13 ng/mL, and the detection limit was 0.033 ng/mL. The visual detection limit of the established colloidal gold immunochromatographic test strip, for the visual detection of actual samples, was 1 ng/g. In summary, this work establishes a rapid detection method of ofloxacin residues on the basis of colloidal gold immunochromatography that is suitable for actual detection. Full article
(This article belongs to the Special Issue State-of-the-Art Technology for Rapid Detection of Pesticide/Chemical Residues in Foods)
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33 pages, 5556 KiB  
Article
Simultaneous Analysis of 504 Pesticide Multiresidues in Crops Using UHPLC-QTOF at MS1 and MS2 Levels
by Mun-Ju Jeong, Su-Min Kim, Ye-Jin Lee, Yoon-Hee Lee, Hye-Ran Eun, Miok Eom, Gui-Hyun Jang, JuHee Lee, Hyeong-Wook Jo, Joon-Kwan Moon and Yongho Shin
Foods 2024, 13(21), 3503; https://doi.org/10.3390/foods13213503 - 31 Oct 2024
Cited by 1 | Viewed by 1551
Abstract
A robust analytical method was developed for the simultaneous detection of 504 pesticide multiresidues in various crops using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF). The method integrates both MS1 and MS2 levels through sequential window acquisition of [...] Read more.
A robust analytical method was developed for the simultaneous detection of 504 pesticide multiresidues in various crops using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF). The method integrates both MS1 and MS2 levels through sequential window acquisition of all theoretical mass spectra (SWATH) analysis, allowing for accurate mass measurements and the construction of a spectral library to enhance pesticide residue identification. An evaluation of the method was carried out according to international standards, including the FAO guidelines and SANTE/11312/2021. Validation across five representative crops—potato, cabbage, mandarin, brown rice, and soybean—demonstrated exceptional sensitivity, with over 80% of the analytes detected at trace levels (≤2.5 μg/kg). Moreover, an impressive 96.8% to 98.8% of the compounds demonstrated LOQs of ≤10 μg/kg. Most compounds exhibited excellent linearity (r2 ≥ 0.980) and satisfactory recovery rates at spiking levels of 0.01 and 0.1 mg/kg. Among 42 crop samples analyzed, pesticides were detected in 1 cabbage, 3 mandarin, and 6 rice samples, with a mass accuracy within ±5 ppm and a Fit score ≥ 70.8, confirming the method’s practical applicability and reliability. The detected residues ranged from 12.3 to 339.3 μg/kg, all below the established maximum residue limits (MRLs). This comprehensive approach offers an efficient, reliable, and scalable solution for pesticide multiresidue monitoring, supporting food safety programs and regulatory compliance. Full article
(This article belongs to the Special Issue State-of-the-Art Technology for Rapid Detection of Pesticide/Chemical Residues in Foods)
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