Aquatic Animal Transgene and Gene Editing Technology

A special issue of Fishes (ISSN 2410-3888). This special issue belongs to the section "Welfare, Health and Disease".

Deadline for manuscript submissions: 20 August 2024 | Viewed by 497

Special Issue Editors


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Guest Editor
Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf Marine Ecological Environment Field Observation and Research Station of Guangxi, College of Marine Sciences, Beibu Gulf University, Qinzhou 535011, China
Interests: RNAi; bacterial disease; aquatic animals

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Guest Editor
College of Fisheries, Guangdong Ocean University, Zhanjiang, China
Interests: diseases control; aquatic animals; RNAi
Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf Marine Ecological Environment Field Observation and Research Station of Guangxi, College of Marine Sciences, Beibu Gulf University, Qinzhou 535011, China
Interests: RNAi; CRISPR/Cas9; TALEN

Special Issue Information

Dear Colleagues,

Aquatic animals are widely distributed in rivers, lakes, and seas, which occupy 70% of the earth's surface. Gene editing is the manipulation of the genome of the organism itself by knocking out or replacing targeted gene which resulting in individuals with intentionally selected and desired traits, while transgenic technology can only introduce biologically nonexisting foreign genes to the original organisms in order to tailor the species with new traits. Performance traits have been altered in aquatic animals via transgenesis, such as the growth hormone gene has been transferred to several species, including Misgurnus anguillicaudatus, Cyprinuscarpio, Atlanticsalmon resulting in 10–3000% faster growth rates compared to non-transgenic fish in aquaculture conditions. Transgenes often produce pleiotropic effects, such as reproduction and viability of progeny, production of the transgenic line offers shorter production times, reduced costs, and improved profitability to aquaculturists. Transgenic mice were firstly generated at 1982, and first transgenic aquatic animals were rainbow trout and goldfish, which were generated at 1985. This Special Issue welcomes original articles and review articles dealing with the Aquatic Animal Transgene and Gene Editing Technology.

Prof. Dr. Youhou Xu
Prof. Dr. Shuanghu Cai
Dr. Peng Zhu
Guest Editors

Manuscript Submission Information

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Keywords

  • transgene
  • gene editing technology (TALEN or CRISPR/Cas9)
  • RNAi
  • freshwater species
  • marine species

Published Papers (1 paper)

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Research

13 pages, 2747 KiB  
Article
Effect of CRISPR/Cas9 Targets Associated with Iron Metabolism and Its Variation on Transcriptional Regulation of SHK-1 Cell Line as a Model for Iron Metabolism
by Phillip Dettleff, Yehwa Jin, Carolina Peñaloza, Rodrigo Pulgar, Alejandro Sáez, Diego Robledo and Sebastian Escobar-Aguirre
Fishes 2024, 9(6), 198; https://doi.org/10.3390/fishes9060198 - 26 May 2024
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Abstract
In this study, we investigated the function of a gene associated with iron metabolism using CRISPR-Cas9 and RNA sequencing in SHK-1 salmon cells. Our objective was to understand how different guide RNA (gRNA) sequences against the transferrin gene tf could influence gene expression [...] Read more.
In this study, we investigated the function of a gene associated with iron metabolism using CRISPR-Cas9 and RNA sequencing in SHK-1 salmon cells. Our objective was to understand how different guide RNA (gRNA) sequences against the transferrin gene tf could influence gene expression and cellular processes related to iron uptake. RNA-Seq analysis was performed to evaluate the transcriptomic effects of two distinct gRNA targets with high knock-out (KO) efficiencies for the targeted tf gene in the SHK-1 genome. Our results showed no significant differential expression in transferrin-related transcripts between wild-type and CRISPR-edited cells; however, there were major differences between their transcriptomes, indicating complex transcriptional regulation changes. Enrichment analysis highlighted specific processes and molecular functions, including those related to the nucleus, cytoplasm, and protein binding. Notably, different sgRNAs targeting tf might result in different mutations at DNA levels in SHK-1 salmon cells. Full article
(This article belongs to the Special Issue Aquatic Animal Transgene and Gene Editing Technology)
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