Application of Fermentation Technology in Animal Nutrition: 3rd Edition

A special issue of Fermentation (ISSN 2311-5637).

Deadline for manuscript submissions: 15 August 2026 | Viewed by 2192

Special Issue Editor

Special Issue Information

Dear Colleagues,

Fermentation technology in animal nutrition has been used by human beings across the globe for a long time. Its technology has primarily been focused on animal feed aspects. Its main purpose was to store feedstuffs for a long-term period. Harvested forages, putrefiable agro-industrial or food processing by-products, and the total mixed rations with these ingredients are often ensiled in order to make them palatable, stable, and sanitary over seasons. Additionally, another purpose was to improve the feed’s nutritional values or safety of underutilized feed resources with fermentation technology; otherwise, their usage as feed may be limited due to low digestion or potential toxicity. To fulfil these goals, various physical, chemical, and microbiological techniques have been developed.

More specifically, many factors affect the fermentation of feedstuffs, such as temperature, moisture, air, nutrients, microbes, acidic condition, and the environmental situation, among others. Various types of additives have been developed to improve the quality of ferments and aerobic stability such as nutrients, microbes, enzymes, organic acids, and others. With these scientific research efforts, fermentation technology has been much developed and utilized in the animal industry. However, we still do not have a complete understanding of the most effective fermentation factors under various conditions, the mechanisms of fermentation products and metabolites’ utilization inside the animal body, and their effect on ruminal fermentation, gastrointestinal microflora and health, animal productivity and health, and final animal products, among others.

In conclusion, we aim for this valuable Special Issue on fermentation in animal nutrition to provide promising scientific answers to these interesting questions.

Dr. Siran Wang
Guest Editor

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Keywords

  • fermentation
  • silage
  • forage
  • by-product
  • feed
  • nutrition
  • animal

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Published Papers (2 papers)

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Research

20 pages, 1660 KB  
Article
A Comparative Study of Cutting Height and Fermentation Method on Cenchrus fungigraminus Silage: Effects of Natural Fermentation Versus Microbial Inoculant on Silage Quality and Fiber Degradation
by Hongyuan He, Ziting Wang, Fuke Hako, Ben Menda Ukii, Jufen Deng, Mengying Zhao, Zhanxi Lin, Peishan He, Jing Li, Simeng Song, Tingdan Liu and Dongmei Lin
Fermentation 2026, 12(2), 115; https://doi.org/10.3390/fermentation12020115 - 16 Feb 2026
Viewed by 807
Abstract
Cenchrus fungigraminus (Juncao) is a high-yielding, fast-growing forage crop with considerable potential for livestock feed; however, optimizing its processing is essential for cost reduction and quality enhancement. This study comprised three components: (1) a comprehensive analysis of 25 on-farm silage samples from five [...] Read more.
Cenchrus fungigraminus (Juncao) is a high-yielding, fast-growing forage crop with considerable potential for livestock feed; however, optimizing its processing is essential for cost reduction and quality enhancement. This study comprised three components: (1) a comprehensive analysis of 25 on-farm silage samples from five locations in Southwest China using Grey Relational Analysis (GRA); (2) an assessment of the effects of three cutting heights (low: 100–150 cm; mid: 150–200 cm; high: 200–250 cm) on silage quality; and (3) a comparison of silage quality between natural fermentation and microbial inoculant treatments using mature Juncao (250–300 cm). The results showed that: (1) in the on-farm silage samples, carbon supplementation was significantly positively correlated with total digestible nutrients (TDN), relative feed value (RFV), ether extract (EE), and sensory evaluation (p < 0.05), and the GRA identified the top-ranked treatments, including J2, J3, J6, X6, and J5; (2) in the cutting height trials, fiber content increased significantly with cutting height (p < 0.05), while crude protein (CP) and TDN decreased significantly (p < 0.05). The 200–250 cm group exhibited optimal fermentation quality, characterized by the highest total volatile fatty acids (total VFA) and lactic acid concentrations, alongside the lowest pH and ammonia nitrogen/total nitrogen ratios (NH3-N/TN); (3) in the inoculant comparison, the natural fermentation group demonstrated significantly higher degradation rates of acid detergent fiber (ADF), neutral detergent fiber (NDF), and acid detergent lignin (ADL) compared to the microbial inoculant group, while also maintaining a lower pH, higher total VFA and lactic acid. Consequently, for on-farm production, carbon supplementation is recommended to improve silage quality. Although cutting Juncao below 200 cm provides higher nutritional value, a height of 200–250 cm is advised to ensure optimal fermentation characteristics. Furthermore, natural fermentation proves superior to microbial inoculant treatment for mature Juncao. Together, these measures offer an effective strategy for producing high-quality Juncao silage. Full article
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20 pages, 707 KB  
Article
Can Pre-Fermented Juice Be an Alternative Probiotic Helping to Reduce Heat Stress in Laying Japanese Quails (Metabolism and Nutrition)
by Sadık Serkan Aydin, Mehmet Avci, Nurcan Kirar, Ahmet Oruç, Mehmet Savrunlu and Aydin Daş
Fermentation 2026, 12(2), 109; https://doi.org/10.3390/fermentation12020109 - 12 Feb 2026
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Abstract
This study aimed to determine the effects of incorporating probiotic fermented natural lactic acid bacteria (PFJ) into the drinking water of laying quails subjected to temperature stress on egg production, egg quality, cecum microbiology, blood parameters, and incubation performance. A total of 260 [...] Read more.
This study aimed to determine the effects of incorporating probiotic fermented natural lactic acid bacteria (PFJ) into the drinking water of laying quails subjected to temperature stress on egg production, egg quality, cecum microbiology, blood parameters, and incubation performance. A total of 260 Japanese quails (Coturnixcoturnix japonica) aged 8 weeks were used, of which 200 were females, and 60 were males. The quails were divided into four groups, each containing 50 individuals, and further split into five subgroups of 10 quails. For every 10 female quails, three male quails were housed together in cages. The experiment was conducted under normal environmental conditions in control, PFJ, heat-stressed control, and heat-stressed PFJ groups. Temperature stress (34–36 °C) was applied for 8 h daily. The results indicated that by the end of weeks 1–4, the feed conversion ratios of all groups were significantly different, with the best feed conversion ratio of 2.36 found in the PFJ group under temperature stress. Throughout the periods of weeks 1–4 and 5–8, there were statistically significant differences (p < 0.01) in the daily average feed consumption and egg weights among all treated groups. Temperature stress and the addition of PFJ significantly affected shell thickness, Haugh units, albumen weight, yolk weight, and yolk color; no significant impacts were observed on egg shape index, yolk percentage (%), albumen percentage (%), and specific gravity (g/cm3). The highest counts of lactic acid bacteria (LAB) were found in the PFJ-treated groups under both normal and temperature-stressed conditions. Under temperature stress, the levels of Enterobacter, coliform, and E. coli decreased with the addition of PFJ. Regarding blood parameters, significant differences (p < 0.05) were observed in total protein values among groups, while differences in chloride, triglycerides, total cholesterol, LDL cholesterol, HDL cholesterol, and triglyceride concentrations were not statistically significant (p > 0.05). Furthermore, the addition of temperature stress and PFJ significantly affected fertility rates, incubation efficiency, and hatchability. In conclusion, these findings suggest that PFJ could be considered a potential probiotic alternative for improving nutrition in laying quails under conditions of temperature stress. Full article
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