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Diagnostic and Prognostic Biomarkers in Prostate Cancer

A special issue of Diagnostics (ISSN 2075-4418). This special issue belongs to the section "Clinical Diagnosis and Prognosis".

Deadline for manuscript submissions: closed (30 September 2025) | Viewed by 916

Special Issue Editor


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Guest Editor
1. Department of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, Italy
2. Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy
Interests: urology oncology

Special Issue Information

Dear Colleagues,

Prostate cancer is one of the most common malignant tumors in men. Due to its high incidence and diagnostic complexity, it has always been a major focus of scientific research and clinical attention. In recent years, there have been developments and improvements in imaging techniques such as mpMRI, microUS, and PSMA PET/CT, which are now widely accepted and utilized. However, biomarkers, which serve as a bridge between disease status and clinical decision making, are leading the precision medicine of prostate cancer into an unprecedented new era. Despite their potential, they are still not widely utilized on a global scale. In this Special Issue, we will explore, in detail, the latest research progress, clinical application value, and future development direction of prostate cancer biomarkers, aiming to provide patients with more accurate and effective treatment strategies.

Dr. Vittorio Fasulo
Guest Editor

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Keywords

  • prostate cancer
  • biomarker
  • biopsy
  • diagnosis
  • prognosis

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Published Papers (1 paper)

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Research

23 pages, 6569 KB  
Article
Characterization and Evaluation of CD24 and NPY as Biomarkers for Metastatic Castration-Resistant Prostate Cancer
by Peter R. McHenry, Najla Fakhruddin, Kevin Homer, Rui M. Gil da Costa, Lawrence D. True and Colm Morrissey
Diagnostics 2026, 16(5), 657; https://doi.org/10.3390/diagnostics16050657 - 25 Feb 2026
Viewed by 350
Abstract
Background/Objectives: Prostate cancer is the most diagnosed and third most deadly cancer among men in Europe. Metastatic castration-resistant prostate cancer (mCRPC) is incurable and resistant to standard androgen ablation therapy. More biomarkers are needed to select patients for novel personalized treatments. Previous [...] Read more.
Background/Objectives: Prostate cancer is the most diagnosed and third most deadly cancer among men in Europe. Metastatic castration-resistant prostate cancer (mCRPC) is incurable and resistant to standard androgen ablation therapy. More biomarkers are needed to select patients for novel personalized treatments. Previous whole-genome RNA sequencing results indicated a possible role for cluster of differentiation 24 (CD24) and neuropeptide Y (NPY) as diagnostic or prognostic biomarkers in androgen receptor-positive (AR+) mCRPC. Methods: We analyzed tissue microarrays representing 127 primary prostate cancers (with matched adjacent benign prostatic glands) and 124 metastases (from 34 patients) using immunohistochemistry to detect CD24 or NPY. Results: CD24 was more highly expressed in primary prostate cancer than in adjacent benign tissue for nuclear (p: <0.001), cytoplasmic (p: <0.001), and membranous staining (p: <0.001), while NPY showed no difference. Average NPY scores were lower in prostate cancers that later recurred (geometric mean 17.6, 95% CI: 9.5–32.5) compared to those that did not (38.7, CI: 23.2–64.4; p: 0.044, d: 0.773). In mCRPC, CD24 was detectable in 76% of cores at the cell membrane and in 58% in the nucleus. NPY was detectable in the cytoplasm of 17%. Scores for NPY and nuclear (but not membranous) CD24 were higher in AR+ mCRPC. In the RNA sequencing results, CD24 did not correlate with AR or kallikrein-related peptidase 3 (KLK3), while NPY positively correlated with AR (rs: 0.313; p: <0.004) and KLK3 (rs: 0.400; p: <0.004). NPY and CD24 scores did not correlate with neuroendocrine mCRPC markers. Nuclear and membranous CD24 showed differential expression by metastatic site. Conclusions: We did not find strong evidence to support the use of CD24 or NPY alone as clinical biomarkers. Membranous and nuclear CD24 were expressed in the majority of mCRPC specimens, while NPY expression was more limited. NPY and nuclear CD24 were more highly expressed in AR+ mCRPC than AR neuroendocrine disease, and nuclear CD24 displayed site-specific expression, suggesting a potential role for nuclear CD24 in promoting AR+ mCRPC. Full article
(This article belongs to the Special Issue Diagnostic and Prognostic Biomarkers in Prostate Cancer)
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