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Molecular Mechanisms of Fruit Development, Stress Response, and Quality Formation in Horticultural Crops

A special issue of Current Issues in Molecular Biology (ISSN 1467-3045). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: 31 December 2026 | Viewed by 1010

Editor


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Guest Editor
Department of Pomology, College of Horticulture, South China Agriculture University, Guangzhou 510640, China
Interests: plant; genetics; vegetables; fruits
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Fruit development in horticultural crops is orchestrated by complex molecular networks. Key phytohormones like auxin, gibberellin, and ethylene regulate cell division and expansion, while transcription factors, such as MADS-box proteins, govern the transition from flower to fruit. Concurrently, plants employ sophisticated mechanisms to counter environmental stresses like drought, salinity, and pathogens. These involve stress-responsive genes, antioxidant systems, and signaling molecules like abscisic acid (ABA) and reactive oxygen species (ROS), which activate protective pathways to maintain cellular homeostasis. Fruit quality—which encompasses flavor, color, texture, and nutritional value—is the integrated outcome of these developmental and stress-response programs. This process requires the precise regulation of metabolic pathways for sugars, acids, pigments (anthocyanins and carotenoids), volatiles, and health-promoting compounds. Crucially, epigenetic modifications and post-transcriptional regulation fine-tune these processes. Understanding these interconnected molecular mechanisms is vital for breeding superior varieties with enhanced resilience, yield, and consumer-desired traits in a changing climate. This Special Issue delves into the molecular symphony of fruit development, exploring how phytohormones and environmental cues orchestrate growth from blossom to harvest. We will also illuminate the sophisticated mechanisms plants employ to build resilience against stress, and how these very processes fundamentally shape the final quality, flavor, nutrition, and appeal of horticultural crops

Dr. Rahat Sharif
Guest Editor

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Keywords

  • phytohormones
  • fruit development
  • abiotic stress
  • pathogen resistance
  • epigenetics
  • metabolic pathways
  • postharvest biology

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Published Papers (2 papers)

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Research

15 pages, 4096 KB  
Article
Metabolomics Reveals the Dynamic Characteristics of Flavonoids During the Different Developmental Stages of Citrus reticulata ‘Shiyue Ju’ and Its Mutant C. reticulata ‘Denglong Ju’
by Qin Guan, Doudou Huang, Lan Zhang and Zongyou Lv
Curr. Issues Mol. Biol. 2026, 48(8), 774; https://doi.org/10.3390/cimb48080774 - 29 Jul 2026
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Abstract
Flavonoids, prominent bioactive compounds in citrus fruits, exhibit diverse health-promoting properties. However, the dynamic compositional changes in flavonoids during fruit development remain insufficiently characterized in Citrus reticulata ‘Shiyue Ju’ (STJ) and C. reticulata ‘Denglong Ju’ (DLJ). In this study, a comprehensive untargeted metabolomics [...] Read more.
Flavonoids, prominent bioactive compounds in citrus fruits, exhibit diverse health-promoting properties. However, the dynamic compositional changes in flavonoids during fruit development remain insufficiently characterized in Citrus reticulata ‘Shiyue Ju’ (STJ) and C. reticulata ‘Denglong Ju’ (DLJ). In this study, a comprehensive untargeted metabolomics approach was employed to systematically analyze the flavonoid profiles in both peel and flesh tissues across six key developmental stages, leading to the putative annotation of 205 flavonoids. Heatmap analysis revealed higher flavonoid levels in SP-4 (STJ peel in stage 4) compared to DP-4 (DLJ peel in stage 4), potentially resulting from the substantial upregulation of genes involved in flavonoid biosynthesis during this period. Furthermore, S-plot analysis identified salvigenin, demethylnobiletin, and nobiletin as key discriminators in the peel of STJ (SP), whereas sinensetin, tangeretin, and 3′,4′,5,7-tetramethoxyflavone were predominant in the peel of DLJ (DP). These compounds could represent potential biochemical markers for varietal differentiation. Notably, SP exhibited higher levels of neohesperidin, hesperidin, naringin, and naringenin, suggesting enhanced health-promoting properties. The results enhance our understanding of flavonoid dynamics during citrus development and provide valuable implications for quality-oriented cultivation and genetic improvement. Full article
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16 pages, 4762 KB  
Article
Time-Resolved Secondary Metabolite Profiling of Seeded and Seedless Ougan at Commercial Harvest Maturity
by Quan Zhao, Peian Zhang, Yang Song, Fayong Li, Yingyao Liu, Jun Chen and Dongfeng Liu
Curr. Issues Mol. Biol. 2026, 48(6), 596; https://doi.org/10.3390/cimb48060596 - 5 Jun 2026
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Abstract
Ougan (Citrus suavissima Hort. et Tanaka) is valued for its distinctive sweet–bitter flavor and nutritional properties; however, tissue-resolved metabolic differences between two cultivar forms (seeded and seedless) of Ougan (C. suavissima) remains poorly understood. In this study, a comprehensive UPLC-MS/MS-based [...] Read more.
Ougan (Citrus suavissima Hort. et Tanaka) is valued for its distinctive sweet–bitter flavor and nutritional properties; however, tissue-resolved metabolic differences between two cultivar forms (seeded and seedless) of Ougan (C. suavissima) remains poorly understood. In this study, a comprehensive UPLC-MS/MS-based metabolomic analysis was conducted on peel (SP and NP), pulp (SF and NF), segment membrane (SM and NM) and seed tissues (SS, from seeded fruit only) of seeded and seedless Ougan fruits. A total of 1333 metabolites were annotated, with flavonoids (48.53%) and phenolics (12.25%) representing the predominant compound classes. Tissues specificity was the primary determinant of metabolic variation, with peel and segment membrane tissue showing relatively high abundance (fold change ≥ 2, |Log2FC| ≥ 1) of phenylpropanoid- and flavonoid-derived metabolites. Comparative analysis between seeded and seedless tissues revealed significant modulation of phenylpropanoid biosynthesis, flavonoid biosynthesis, phenylalanine metabolism, and related secondary metabolite pathways. Seeded tissues showed a higher relative abundance of selected flavonol glycosides (6-hydroxykaempferol-3,6-O-diglucoside), hydroxycinnamic acid derivatives, and santhocyanin-related compounds, whereas seedless tissues showed higher relative abundance of selected flavanones and malonylated flavonoid glycosides. Seeds were characterized by high limonin content, consistent with limonoid-associated bitterness chemistry. Overall, our findings provide a tissue-resolved metabolomic framework for understanding quality-associated secondary metabolite variation in mature Ougan fruit. Full article
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