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Biomaterials for Disease Diagnosis and Therapy: Emerging Molecular Innovations and Applications

A special issue of Current Issues in Molecular Biology (ISSN 1467-3045). This special issue belongs to the section "Biochemistry, Molecular and Cellular Biology".

Deadline for manuscript submissions: 31 January 2027 | Viewed by 849

Editor


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Guest Editor
LSU Health Shreveport, 1501 Kings Highway, Shreveport, LA 71103, USA
Interests: biomaterials; molecular diagnostics; targeted therapy; drug delivery; nanomaterials

Special Issue Information

Dear Colleagues,

Recently, the rapid evolution of biomaterials has transformed precision medicine by enabling early diagnosis and the development of personalized therapies. Thanks to recent cutting-edge advancements in biomaterials for disease diagnosis and treatment, both natural and synthetic biomaterials have garnered significant attention for their applications in medical sciences, aiming to improve patient outcomes and alleviate healthcare burdens. Therefore, this Special Issue highlights advancements in biomaterials for disease diagnosis and therapy, with a particular emphasis on molecular innovations that enhance accuracy, efficiency, and clinical applicability.

We invite original research and reviews on innovative biomaterial designs, including hydrogels, scaffolds, nanoparticles, and bioengineered molecules, for diagnostic applications such as biosensors, organ-on-a-chip, and imaging agents, as well as therapeutic applications, including drug delivery systems, tissue engineering (3D and 4D printing), and immunotherapy. The focus will be on biocompatibility, molecular mechanisms, and translational potential to address unmet clinical needs in conditions such as wound healing, cancer, and neurological disorders.

Dr. Md Tipu Sultan
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-anonymized peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Current Issues in Molecular Biology is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biomaterials
  • disease diagnosis
  • nanoparticles
  • hydrogels
  • scaffolds
  • bio 3D printing
  • bioink
  • biosensors
  • drug delivery
  • tissue engineering
  • biocompatibility
  • translational medicine
  • cancer
  • neurodegenerative disorders
  • disease model
  • 3D-printed microfluidic devices
  • organ on a chip
  • 3D-printed medical devices

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Published Papers (1 paper)

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Research

11 pages, 461 KB  
Article
Colloidal Light Scattering Distorts Fluorometric Quantification of mRNA in Lipid Nanoparticles
by Agnieszka Klusek, Kamil Adasiewicz, Andżelika Bystrzejewska, Piotr J. Rudzki, Maciej Wieczorek and Ewelina Juszczyk
Curr. Issues Mol. Biol. 2026, 48(8), 805; https://doi.org/10.3390/cimb48080805 - 9 Aug 2026
Viewed by 360
Abstract
Accurate measurement of messenger RNA (mRNA) concentration and encapsulation efficiency remains a challenging task during mRNA-lipid nanoparticle (LNP) formulation development and quality control. RiboGreen-based fluorescence assays are often used for this purpose. LNP formulations are colloidal systems and may exhibit turbidity, which can [...] Read more.
Accurate measurement of messenger RNA (mRNA) concentration and encapsulation efficiency remains a challenging task during mRNA-lipid nanoparticle (LNP) formulation development and quality control. RiboGreen-based fluorescence assays are often used for this purpose. LNP formulations are colloidal systems and may exhibit turbidity, which can interfere with fluorescence detection. The impact of LNP-related optical properties on assay performance remains insufficiently characterized. This study aimed to evaluate whether and how physicochemical factors associated with LNP-based drug-delivery systems influence fluorometric mRNA quantification. Empty LNPs, with the same lipid composition as mRNA-loaded LNPs, were used as a model matrix causing turbidity. Their effect on the RiboGreen fluorescence signal was assessed following dilution and then in different scenarios in the presence of free and encapsulated mRNA. The UV–Vis spectrum of the placebo dispersion was recorded to examine possible optical interference in the measurement range. We quantitatively characterized the suppression of fluorescence signals in the presence of LNPs in a concentration-dependent manner. These findings indicate that turbidity and LNP-associated matrix effects can bias RiboGreen-based mRNA quantification. To ensure reliable determination of mRNA concentration and accurate calculation of encapsulation efficiency in LNP formulations, method-specific dilution factors should be applied. Full article
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