Insight into Developments and Applications of Flow Cytometry
A special issue of Cells (ISSN 2073-4409).
Deadline for manuscript submissions: closed (30 November 2024) | Viewed by 1068
Special Issue Editors
Interests: spectral flow cytometry; fluorochromes; panel design; tumor immunology; extracellular vesicles; cardiovascular diseases
Interests: flow cytometry and cell sorting; immunology; oncohematology
Special Issue Information
Dear Colleagues,
Flow cytometry has become one of the most widespread technologies used for the characterization of a variety of biological samples, as it provides detailed information on cell/particle features such as size, granularity, and antigen expression.
Over the last few decades, flow cytometry’s versatility has led to its adoption into various fields of investigation, including immunology, hematology, oncology, gene therapy, vaccine development, microbiology, and infectious diseases.
Recent developments in this technique have expanded its capabilities. For instance, the introduction of spectral flow cytometry, which allows for the simultaneous detection of a larger quantity of fluorochromes with minimal spectral overlap, has enhanced resolution and multiplexing capacity for deeper and more complex flow cytometric analyses, even in sample with limited availability. Furthermore, the integration of flow cytometry with other technologies, such as single-cell RNA sequencing (CITE-seq) and microscopy (imaging cytometry), together with cell sorting, has facilitated the comprehensive multi-omics profiling of individual cells, offering deeper insights into cellular heterogeneity and function.
The rampant evolution of flow cytometry has been playing a key role in driving advancements in both basic and translational research as well as in clinical diagnostics, thus leading to a better understanding of cellular biology and disease pathogenesis.
The aim of this Special Issue is to collect recent advances in flow cytometry, including new frontiers of flow cytometry applications as well as any new standardization procedures aimed at enhancing data reliability and reproducibility.
Dr. Achille Anselmo
Dr. Federico Simone Colombo
Dr. Veronica Nash
Guest Editors
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Keywords
- flow cytometry
- mass cytometry
- spectral flow cytometry
- fluorochromes
- autofluorescence
- standardization procedures
- imaging flow cytometry
- immunophenotype
- immunology
- oncohematology
- high-dimensional analysis
- cell sorting
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