Glycosylation: From Molecules to Organisms

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Cellular Pathology".

Deadline for manuscript submissions: closed (15 July 2023) | Viewed by 1903

Special Issue Editors


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Guest Editor
Centre for Organismal Studies (COS), Department Glycobiology, Heidelberg University, 69120 Heidelberg, Germany
Interests: protein glycosylation; mannosyltransferases; O-mannosyl glycans; congenital disorders of glycosylation; endoplasmic reticulum
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Guest Editor
Institute of Clinical Biochemistry, Hannover Medical School (MHH), Carl-Neuberg-Strasse 1, 30625 Hannover, Germany
Interests: glycomics; C-mannosylation; glycosphingolipids; congenital disorders of glycosylation; human pluripotent stem cells; proteomics

Special Issue Information

Dear Colleagues,

Glycosylation is a highly future-oriented research area in life sciences. Protein- and lipid-linked carbohydrates influence many fundamental molecular cellular processes, such as protein folding and stability, subcellular localization, cell signaling, and cell adhesion to name just a few. Thus, as might be expected, glycoconjugates affect virtually all aspects of growth and development in eukaryotes. A huge variety of genetic defects in the synthesis and attachment of glycans to proteins and lipids—mostly termed congenital disorders of glycosylation (CDG)—are now known, covering a broad clinical spectrum and affecting almost every organ system. This Special Issue will cover a broad range of studies on biosynthesis and function of glycosylation in eukaryotes, and insights into the implications of aberrant glycosylation at the molecular, cellular and organismal levels ranging from yeast to human disease models.

It is our pleasure to invite you to submit an article for a Special Issue of the journal Cells on the subject of “Glycosylation: from molecules to organisms”. The article may be either a full paper or a communication based on your own research or a focused review article on some aspect of the subject.

Prof. Dr. Sabine Strahl
Dr. Falk F. Buettner
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • glycosylation
  • N-glycosylation
  • O-glycosylation
  • C-mannosylation
  • glypiation
  • glycan
  • glycolipids
  • glycomics
  • glycoproteomics
  • glycoprotein
  • glycosyltransferase
  • dolichol
  • lipid linked oligosaccharide
  • sugar nucleotide transport
  • endoplasmic reticulum
  • Golgi apparatus
  • protein maturation
  • cellular trafficking
  • cell–cell interaction
  • cell–matrix interaction
  • congenital disorders of glycosylation
  • dystroglycanopathy
  • fungal pathogenicity
  • innate immunity
  • immunology
  • cancer

Published Papers (1 paper)

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Research

18 pages, 11417 KiB  
Article
Isotopic Tracing of Nucleotide Sugar Metabolism in Human Pluripotent Stem Cells
by Federica Conte, Marek J. Noga, Monique van Scherpenzeel, Raisa Veizaj, Rik Scharn, Juda-El Sam, Chiara Palumbo, Frans C. A. van den Brandt, Christian Freund, Eduardo Soares, Huiqing Zhou and Dirk J. Lefeber
Cells 2023, 12(13), 1765; https://doi.org/10.3390/cells12131765 - 03 Jul 2023
Cited by 1 | Viewed by 1479
Abstract
Metabolism not only produces energy necessary for the cell but is also a key regulator of several cellular functions, including pluripotency and self-renewal. Nucleotide sugars (NSs) are activated sugars that link glucose metabolism with cellular functions via protein N-glycosylation and O-GlcNAcylation. Thus, understanding [...] Read more.
Metabolism not only produces energy necessary for the cell but is also a key regulator of several cellular functions, including pluripotency and self-renewal. Nucleotide sugars (NSs) are activated sugars that link glucose metabolism with cellular functions via protein N-glycosylation and O-GlcNAcylation. Thus, understanding how different metabolic pathways converge in the synthesis of NSs is critical to explore new opportunities for metabolic interference and modulation of stem cell functions. Tracer-based metabolomics is suited for this challenge, however chemically-defined, customizable media for stem cell culture in which nutrients can be replaced with isotopically labeled analogs are scarcely available. Here, we established a customizable flux-conditioned E8 (FC-E8) medium that enables stem cell culture with stable isotopes for metabolic tracing, and a dedicated liquid chromatography mass-spectrometry (LC-MS/MS) method targeting metabolic pathways converging in NS biosynthesis. By 13C6-glucose feeding, we successfully traced the time-course of carbon incorporation into NSs directly via glucose, and indirectly via other pathways, such as glycolysis and pentose phosphate pathways, in induced pluripotent stem cells (hiPSCs) and embryonic stem cells. Then, we applied these tools to investigate the NS biosynthesis in hiPSC lines from a patient affected by deficiency of phosphoglucomutase 1 (PGM1), an enzyme regulating the synthesis of the two most abundant NSs, UDP-glucose and UDP-galactose. Full article
(This article belongs to the Special Issue Glycosylation: From Molecules to Organisms)
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