Cell-Based Biosensors for Rapid Detection and Monitoring (3rd Edition)

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: 31 August 2026 | Viewed by 881

Special Issue Editor


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Guest Editor
School of Applied Biology and Biotechnology, Agricultural University of Athens, 11855 Athens, Greece
Interests: cell-based biosensors; cell and neuronal differentiation; real-time monitoring systems; high-throughput screening or diagnostics systems
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Special Issue Information

Dear Colleagues,

Cells, as biorecognition elements, have many advantages, such as sensitivity and rapid response to various stimuli. During the last decade, researchers have achieved progress in improving the selectivity of cells against desired target analytes as well as their integration into biosensors, which has led to their use in many applications. For this reason, cell-based biosensors represent one of the most advanced and, at the same time, challenging scientific and technological domains in analytical and diagnostic sciences. The most popular application is in toxicology research but due to selectivity improvement, they are also used as analytical devices for specific molecules. This Biosensors Special Issue on “Cell-Based Biosensors for Rapid Detection and Monitoring” is intended to be a timely and comprehensive Special Issue on very recent and emerging technologies in the fascinating field of cell-based biosensors for the rapid detection of molecules such as biomarkers, environmental pollutants, etc., and/or the monitoring of cell physiology in response to pharmacological or environmental stimuli. Topics include, but are not restricted to, cell-based methodological approaches, synthetic cell manufacturing (targeted genome editing, genetic circuits, membrane engineering, etc.), integration of cell-based biosensors into platforms, and current applications and perspectives for cell-based biosensors and analytical devices for toxicology and drug research, such as lab-on-a-chip or organ-like cultures. Research papers, short communications, and reviews are all welcome.

Dr. Georgia Moschopoulou
Guest Editor

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Keywords

  • analytical devices
  • bioelectric
  • cell-based biosensors
  • enviromental pollutants
  • food safety
  • impedimetric biosensors
  • medical diagnostics
  • microbial fuel cells
  • toxicology
  • synthetic cells

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Published Papers (2 papers)

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Research

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18 pages, 1868 KB  
Article
Cell-Based Luciferase Assay for Testing SARS-CoV-2 3CL Protease Inhibitors
by Dmitry N. Shcherbakov, Ekaterina D. Mordvinova, Vadim O. Trufanov, Natalia V. Volkova, Yulia V. Meshkova, Maria K. Marenina, Anna V. Zaykovskaya, Ekaterina A. Volosnikova, Sophia S. Borisevich and Svetlana V. Belenkaya
Biosensors 2026, 16(5), 253; https://doi.org/10.3390/bios16050253 - 30 Apr 2026
Viewed by 393
Abstract
A cell-based screening system for viral protease inhibitors was developed using firefly luciferase fragment complementation and validated on the SARS-CoV-2 3CLpro model. The optimal luciferase variant incorporating the VLQSGF proteolytic site (Luc III) retained 88% of its native activity. A critical requirement for [...] Read more.
A cell-based screening system for viral protease inhibitors was developed using firefly luciferase fragment complementation and validated on the SARS-CoV-2 3CLpro model. The optimal luciferase variant incorporating the VLQSGF proteolytic site (Luc III) retained 88% of its native activity. A critical requirement for system performance was the use of an extended nsp4–nsp6 fragment of the viral polyprotein rather than the mature protease, underscoring the importance of the native context for 3CLpro activity. The bicistronic construct pCAG-Luc-III-IRES-nsp4-6 enables coordinated expression of the reporter and protease, thereby increasing assay reproducibility. IC50 values obtained in this system for nirmatrelvir and GC376 correlated with live-virus assay data but differed significantly from those of a cell-free FRET assay, reflecting the impact of cellular barriers. This approach combines simplicity, a standard substrate, and high reproducibility, making it promising for high-throughput screening in basic laboratory settings and adaptable to other viral proteases. Full article
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24 pages, 1056 KB  
Review
Cell-Based Biosensors in Oral Health: Emerging Tools for Rapid Detection and Monitoring of Oral Diseases
by Florinel Cosmin Bida, Ionut Luchian, Dana Gabriela Budala, Dragos Ioan Virvescu, Costin Iulian Lupu, Oana Maria Butnaru, Teona Tudorici, Florin Razvan Curca, Ovidiu Aungurencei and Andrei Georgescu
Biosensors 2026, 16(5), 254; https://doi.org/10.3390/bios16050254 - 30 Apr 2026
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Abstract
Oral diseases remain highly prevalent worldwide and require early diagnosis and continuous monitoring to improve clinical outcomes. Conventional diagnostic methods are often invasive, time-consuming, and limited in their capacity for real-time assessment, which has driven the development of biosensor technologies for point-of-care applications. [...] Read more.
Oral diseases remain highly prevalent worldwide and require early diagnosis and continuous monitoring to improve clinical outcomes. Conventional diagnostic methods are often invasive, time-consuming, and limited in their capacity for real-time assessment, which has driven the development of biosensor technologies for point-of-care applications. Among these, cell-based biosensors utilize living cells as sensing elements capable of responding to inflammatory mediators, bacterial toxins, metabolic products, and tumor-associated biomarkers. This narrative review summarizes the principles, cell types, detection mechanisms, and applications of cell-based biosensors in oral health. The literature was identified through a structured search of PubMed, Scopus, Web of Science, and Google Scholar using keywords related to cell-based biosensors, oral diagnostics, salivary biomarkers, periodontal disease, oral cancer, and lab-on-chip technologies. Due to the heterogeneity of biosensor designs and detection methods, the selected studies were analyzed qualitatively. Cell-based biosensors have demonstrated applications in periodontal disease detection, cariogenic biofilm monitoring, oral cancer diagnostics, cytotoxicity testing of dental materials, and salivary biomarker analysis. The integration of microfluidic and lab-on-chip systems enables real-time and multiplex detection, supporting the development of chairside diagnostic platforms in dentistry. However, challenges related to standardization, reproducibility, and clinical validation remain and must be addressed to facilitate broader implementation in routine practice. Full article
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