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Biosensors
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Biosensors for Monitoring and Diagnostics, 2nd Edition
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Biosensors for Monitoring and Diagnostics, 2nd Edition

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: 31 March 2027 | Viewed by 4760

Special Issue Editors

Prof. Dr. Radivoje Prodanović

E-Mail Website
Guest Editor
Faculty of Chemistry, University of Belgrade, Belgrade, Serbia
Interests: biocatalysis; protein engineering; biotechnology; high-throughput screening; hydrogels; biocomposites; microfluidics; biosensors; biofuel cells
Special Issues, Collections and Topics in MDPI journals
Dr. Dalibor M. Stanković

E-Mail Website
Guest Editor
Faculty of Chemistry, University of Belgrade, Studentski Trg 12, 11000 Belgrade, Serbia
Interests: materials and composites; electrochemistry; catalysis; nanomaterials; hybrid systems
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue, titled “Biosensors for Monitoring and Diagnostics”, encompasses the critical role of biosensors in advancing healthcare practices. Specifically focusing on electrochemical and optical sensors based on different recognition systems, this research will explore their significance in disease monitoring and diagnosis. This compilation of research and findings explores the potential of electrochemical and optical biosensors to enable the rapid and accurate detection of biomarkers associated with various health conditions. This Special Issue addresses technological advancements and innovations in the engineering of biological components and the design of electrochemical biosensors, highlighting their sensitivity, selectivity, and real-time monitoring capabilities. The main topics include the use of engineering components to facilitate electron transfer and recognition for the immobilization and construction of biosensors, increasing sensitivity, selectivity, and stability. This Special Issue will also cover the role of artificial intelligence and bioinformatics in designing the biological components of biosensors. It will also explore the integration of electrochemical/optical biosensors with emerging technologies, such as wearable devices and point-of-care systems, to facilitate seamless and widespread deployment for monitoring and diagnosis. Overall, this Special Issue will serve as a comprehensive resource for understanding the current landscape and future prospects of electrochemical/optical biosensors in healthcare applications.

Prof. Dr. Radivoje Prodanović
Dr. Dalibor M. Stanković
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • electrochemical sensors
  • optical sensors
  • biomarkers
  • sensing technologies
  • recognition elements
  • engineering

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (3 papers)

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Research

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25 pages, 4839 KB  
Article
Modeling an SPR Sensor for Carcinoma-Related Refractive-Index Detection: The Case of CaF2/Au/Si3N4/BP Multilayer System
by Talia Tene, Martha Ximena Dávalos Villegas and Cristian Vacacela Gomez
Biosensors 2026, 16(4), 198; https://doi.org/10.3390/bios16040198 - 1 Apr 2026
Viewed by 608
Abstract
A thin-film surface plasmon resonance (SPR) sensor is presented using a prism-coupled Kretschmann configuration and an optimized multilayer architecture incorporating black phosphorus (BP) as an ultrathin overlayer. The response is modeled at 633 nm under TM polarization using the transfer-matrix method. Low-concentration sensing [...] Read more.
A thin-film surface plasmon resonance (SPR) sensor is presented using a prism-coupled Kretschmann configuration and an optimized multilayer architecture incorporating black phosphorus (BP) as an ultrathin overlayer. The response is modeled at 633 nm under TM polarization using the transfer-matrix method. Low-concentration sensing conditions in the 1–5 ng/mL range are represented through small effective-refractive-index perturbations of the aqueous sensing medium, providing a preliminary optical framework for evaluating refractive-index response in biosensing-related scenarios. The coupling prism, Au film thickness, and Si3N4 spacer thickness are optimized to control resonance depth, linewidth, and angular shift. The optimized CaF2/Au/Si3N4/BP configuration exhibits systematic condition-dependent displacement of the SPR minimum and an evanescent-field distribution that remains strongly localized at the sensing interface while extending into the sensing medium, enabling refractive-index interrogation. High angular sensitivity is obtained at low levels, reaching 517.62°/RIU at 2 ng/mL and 482.82°/RIU at 1 ng/mL, with quality factors above 120 RIU−1 in the same regime. Composite indicators (figure of merit and contrast signal factor) peak at intermediate levels, whereas resonance broadening at higher levels reduces the quality factor and increases the inferred limit of detection, evidencing a sensitivity–resolution trade-off. Benchmarking against reported SPR platforms indicates that BP-assisted interface engineering provides a competitive low-level operating window within a preliminary refractive-index-sensing framework that is relevant to future biosensor design. These results motivate further experimental validation, including BP stabilization, surface biofunctionalization, and practical implementation under liquid-phase sensing conditions. Full article
(This article belongs to the Special Issue Biosensors for Monitoring and Diagnostics, 2nd Edition)
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Review

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46 pages, 14713 KB  
Review
Challenges of Wearable Biosensors and Ways to Overcome Them
by Sergei Tarasov, Yulia Plekhanova, Anatoly Reshetilov, Sergey Melenkov and Ivan Saltanov
Biosensors 2026, 16(3), 159; https://doi.org/10.3390/bios16030159 - 13 Mar 2026
Viewed by 1994
Abstract
In the 21st century, there have been radical changes in healthcare related to the transition from a universal approach to personalized medicine based on the unique characteristics of each patient. In large part, this has become possible due to the development and distribution [...] Read more.
In the 21st century, there have been radical changes in healthcare related to the transition from a universal approach to personalized medicine based on the unique characteristics of each patient. In large part, this has become possible due to the development and distribution of wearable medical devices that are capable of providing continuous monitoring of a variety of physiological parameters outside medical institutions. The most important of these devices are modern biosensors that allow real-time tracking of various biomarkers in the body, thereby opening up new opportunities for disease prevention, early diagnosis, and personalized treatment strategies. The most obvious example of the transformation is the implementation of wearable devices for continuous glucose monitoring (CGM), which has significantly facilitated the daily lives of millions of people with diabetes. Nevertheless, despite the examples of successful implementation of these devices, their large-scale distribution is associated with many challenges, such as the need for standardization, data transmission security, and the risks of immune responses to implantable devices or infections. This review examines all the current problems of wearable biosensors and possible ways to overcome them. Special emphasis will be placed on devices for continuous glucose monitoring as the most commercially successful representatives of this device class. Full article
(This article belongs to the Special Issue Biosensors for Monitoring and Diagnostics, 2nd Edition)
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Other

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66 pages, 9708 KB  
Systematic Review
Recent Advances in Electrochemical Detection of Antibiotics on Graphene-Based Sensors and Biosensors, Impact and Sustainable Development Challenges: A Systematic Review and Meta-Analysis
by Muhammad Saqib, Mrinal Vashisth, Elena I. Korotkova, Amrit L. Hui, Stephen O. Aremu, Souvik Das, Aniruddha Deb, Nirmal K. Hazra, Rachita Saha, Subrata Saha and Pradip Kumar Kar
Biosensors 2026, 16(5), 234; https://doi.org/10.3390/bios16050234 - 23 Apr 2026
Viewed by 1638
Abstract
The increasing use of antibiotics around the globe has contributed to an increase in antimicrobial resistance and become a major risk to both public health and sustainable development. Reliable and fast detection of antibiotic residues in clinical, agricultural, and environmental matrices is required [...] Read more.
The increasing use of antibiotics around the globe has contributed to an increase in antimicrobial resistance and become a major risk to both public health and sustainable development. Reliable and fast detection of antibiotic residues in clinical, agricultural, and environmental matrices is required to monitor antimicrobial resistance effectively. The conventional analytical techniques are sensitive, but they are also expensive, complex and lacking in portability. Voltammetry is a recently emerging electrochemical detection technique that is low-cost and rapid. To the best of our knowledge, for the first time, a meta-analysis was conducted on graphene-based electrochemical sensors and biosensors for antibiotic detection over the last decade. This systematic review critically examines the analytical properties of sensors and biosensors, the physicochemical properties of antibiotics, adsorption characteristics, and the use of nanoparticles to improve the selectivity and sensitivity of devices. This review critically examines the cost-effectiveness, scalability, and practicality of point-of-use devices using graphene-based sensors and biosensors. This systematic review also discusses the potential risks to human health from antibiotic contamination and the role of monitoring in contributing to achieving the UN’s Sustainable Development Goals. This systematic review identifies a gap between developing sensors in laboratories versus their deployment as field-deployable devices; it highlights challenges associated with stability, matrix effects and the complexity of manufacturing devices. Finally, it provides recommendations for future research that may help to address this gap to promote the transition of innovative devices from academic to practical applications. Full article
(This article belongs to the Special Issue Biosensors for Monitoring and Diagnostics, 2nd Edition)
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