Biochar-Based Electrochemical (Bio)sensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (31 December 2024) | Viewed by 1381

Special Issue Editors


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Guest Editor
Department of Chemical Sciences and Technologies, University of Rome “Tor Vergata”, Via della Ricerca Scientifica, 00133 Rome, Italy
Interests: screen-printed electrode; biosensor; tyrosinase; immunosensor

Special Issue Information

Dear Colleagues,

Biochar is a charcoal material, which has gained attention for its diverse applications, including soil improvement, carbon sequestration, and water filtration. However, its potential as an electrochemical enhancer is an emerging area of research with promising implications. Biochar possesses unique electrochemical properties due to its porous structure and high surface area. These characteristics facilitate electron transfer processes and provide numerous active sites for chemical reactions to occur. Biochar-based electrodes have been explored for the development of electrochemical sensors due to their sensitivity to specific analytes. The high surface area and porous structure of biochar can facilitate the immobilization of biomolecules or other recognition elements, enabling the detection of target molecules with high sensitivity and selectivity.

This Special Issue covers the latest advances in biochar-based electrochemical (Bio)sensors development, focusing on all aspects of design, fabrication, and implementation strategies.

Dr. Rocco Cancelliere
Dr. Laura Micheli
Guest Editors

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Keywords

  • biochar
  • biochar-modified electrodes
  • biochar-based sensors
  • biochar-based immunosensors
  • biochar-based DNA sensors

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Published Papers (1 paper)

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Research

17 pages, 3839 KiB  
Article
An Unlabeled Electrochemical Immunosensor Uses Poly(thionine) and Graphene Quantum Dot-Modified Activated Marigold Flower Carbon for Early Prostate Cancer Detection
by Suparat Cotchim, Supatinee Kongkaew, Panote Thavarungkul, Proespichaya Kanatharana and Warakorn Limbut
Biosensors 2024, 14(12), 589; https://doi.org/10.3390/bios14120589 - 2 Dec 2024
Viewed by 880
Abstract
The activated carbon from marigold flowers (MG) was used to make an unlabeled electrochemical immunosensor to determine prostate cancer. MG was synthesized by hydrothermal carbonization and pyrolysis. MG had a large surface area, was highly conductive, and biocompatible. MG modified with graphene quantum [...] Read more.
The activated carbon from marigold flowers (MG) was used to make an unlabeled electrochemical immunosensor to determine prostate cancer. MG was synthesized by hydrothermal carbonization and pyrolysis. MG had a large surface area, was highly conductive, and biocompatible. MG modified with graphene quantum dots produced excellent electron transfer for grafting poly(thionine) (PTH). The amine group of PTH bonded with anti-prostate-specific antigen (Anti-PSA) via glutaraldehyde, forming a layer that improved electron transfer. The binding affinity of the immunosensor, presented as the dissociation constant (Kd), was calculated using the Langmuir isotherm model. The results showed that a lower Kd value indicated greater antibody affinity. The immunosensor exhibited two different linear ranges under optimized conditions: 0.0125 to 1.0 ng mL−1 and 1.0 to 80.0 ng mL−1. The sensor could detect concentrations as low as 0.005 ng mL−1, and had a quantification limit of 0.017 ng mL−1. This immunosensor accurately quantified PSA levels of human serum, and the results were validated using enzyme-linked fluorescence assay (ELFA). Full article
(This article belongs to the Special Issue Biochar-Based Electrochemical (Bio)sensors)
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