Electrochemical Biosensors for Rapid and Sensitive Detection

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: 31 October 2026 | Viewed by 1230

Special Issue Editors

School of Chemistry and Materials, Yangzhou University, Yangzhou 225002, China
Interests: electrochemiluminescence; electrochemistry; biosensors; biomarkers
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Guest Editor
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
Interests: optical/electro-biosensing; imaging analysis; nanobiomedicine

Special Issue Information

Dear Colleagues,

The pursuit of rapid, highly sensitive and specific detection platforms is a central focus in modern bioanalytical science. This Special Issue aims to highlight cutting-edge research and review articles on the development and application of advanced electrochemical biosensors. We welcome contributions that explore innovative strategies, particularly those integrating novel signal transduction mechanisms such as electrochemiluminescence (ECL) and materials with unique photophysical properties like those exhibiting aggregation-induced emissions (AIEs). The synergistic use of functional nanomaterials to enhance sensor performance—by improving electrical conductivity, increasing surface area and facilitating biomolecule immobilization—is of significant interest.

A key application area for these biosensors is the precise and early detection of critical disease biomarkers in clinical diagnostics, point-of-care testing and environmental monitoring. We invite original research and comprehensive reviews that address the design, fabrication, mechanistic studies and practical analytical applications of these next-generation biosensors. By bringing together diverse expertise, this Special Issue seeks to foster discussion and advance the frontiers of electrochemical sensing technology for improved healthcare and bioanalysis.

Dr. Hang Gao
Prof. Dr. Hong Zhou
Guest Editors

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Keywords

  • electrochemiluminescence
  • aggregation-induced emission
  • biosensors
  • nanomaterials
  • disease biomarkers

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Published Papers (1 paper)

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Research

10 pages, 1034 KB  
Communication
Highly Sensitive Electrochemiluminescence Analysis of miRNA-107 Using AIE-Active Polymer Dots as Emitters
by Zhi-Hong Xu, Xin Weng, Ruo-Mei Lin, Hui Tong, Yang Guo, Li-Shuang Yu, Hang Gao and Qin Xu
Biosensors 2026, 16(2), 99; https://doi.org/10.3390/bios16020099 - 4 Feb 2026
Viewed by 805
Abstract
The ultrasensitive detection of microRNA-17 (miRNA-107) is required for clinical diagnosis. In this work, an aggregation-induced electrochemiluminescence (AIECL) sensor was developed for the quantification of miRNA-107, in which AIECL-active polymer dots (Pdots) were characterized by transmission electron microscopy, ultraviolet–visible spectroscopy, and cyclic voltammetry [...] Read more.
The ultrasensitive detection of microRNA-17 (miRNA-107) is required for clinical diagnosis. In this work, an aggregation-induced electrochemiluminescence (AIECL) sensor was developed for the quantification of miRNA-107, in which AIECL-active polymer dots (Pdots) were characterized by transmission electron microscopy, ultraviolet–visible spectroscopy, and cyclic voltammetry and used as ECL emitters. Black hole quencher-labeled hairpin DNA (HP-BHQ) was modified on the Pdot surfaces, resulting in the ECL signal of the Pdots being in the “off” state due to the resonant energy transfer (RET) between the BHQ and Pdots. In the presence of miRNA-107, HP-BHQ opened through RNA-DNA hybridization. Subsequently, the introduced duplex-specific nuclease (DSN) facilitated the cleavage of DNA in the RNA–DNA hybrid chain and led to the detachment of HP-BHQ from the electrode surface. The ECL signal of the Pdots recovered, i.e., to the “on” state. The variation in the ECL signal was related to the concentration of the target miRNA-107. As a result, the AIECL biosensor exhibited a wide linear response to miRNA-107 concentrations ranging from 1.0 fM to 10.0 pM, and a low detection limit of 0.82 fM. This work provides a novel platform for the sensitive analysis of miRNA. Full article
(This article belongs to the Special Issue Electrochemical Biosensors for Rapid and Sensitive Detection)
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