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Biosensors
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Nanomaterial-Based Biosensors for Point-of-Care Testing
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Nanomaterial-Based Biosensors for Point-of-Care Testing

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: 30 November 2025 | Viewed by 2241

Special Issue Editors

Dr. Samir Kumar

E-Mail Website
Guest Editor
Department of Electronics and Information Engineering, Korea University, Sejong 30019, Republic of Korea
Interests: nano/bio-photonics; SERS; nanofabrication; bio/chemical sensors
Special Issues, Collections and Topics in MDPI journals
Dr. Satyendra Kumar Mishra

E-Mail Website
Guest Editor
Centre Tecnològic de Telecomunicacions de Catalunya, Barcelona, Spain
Interests: quantum communication; fiber and waveguide communication; free space optics communication; quantum sensors
Special Issues, Collections and Topics in MDPI journals
Dr. Sachin Gupta

E-Mail Website
Guest Editor
Department of Physics, Bennett University, Greater Noida 201310, Uttar Pradesh, India
Interests: functional materials; sensors; thin films; quantum materials

Special Issue Information

Dear Colleagues,

We are pleased to invite contributions to this Special Issue concerning “Nanomaterial-Based Biosensors for Point-of-Care Testing”. In healthcare diagnostics, we have seen growing demand for rapid, precise, and easily accessible testing methods. As a result, nanomaterial-based biosensors have emerged as a promising technology. Developing nanomaterial-based biosensors can revolutionize healthcare diagnostics by enabling earlier disease detection, facilitating more targeted treatments, and reducing healthcare costs. The integration of nanomaterials allows for the miniaturization and portability of biosensor devices, making them well suited to point-of-care settings.

This Special Issue will explore the transformative impact of various nanomaterials, including gold and silver nanoparticles, quantum dots, and carbon nanomaterials, on point-of-care testing (POCT). This Special Issue will also explore various readout modalities that complement nanomaterial-based biosensors, including fluorescence, surface plasmon resonance, surface-enhanced Raman scattering, and electrochemical, piezoelectric, magnetic, thermal, and colorimetric detection.

This Special Issue "Nanomaterial-Based Biosensors for Point-of-Care Testing" will provide a comprehensive overview of recent state-of-the-art trends in this rapidly evolving field. In bringing together cutting-edge research and innovative technologies, we will highlight the tremendous potential of nanomaterials to revolutionize POCT and advance personalized healthcare.

Dr. Samir Kumar
Dr. Satyendra Kumar Mishra
Dr. Sachin Gupta
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nanomaterials
  • point-of-care testing (POCT)
  • gold/silver nanoparticles
  • quantum dots
  • carbon nanomaterials
  • optical biosensors
  • electrochemical biosensors
  • piezoelectric biosensors
  • magnetic biosensors
  • colorimetric biosensors

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Published Papers (2 papers)

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Research

12 pages, 3330 KB  
Communication
Exploration of the Tolerance of Novel Coronaviruses to Temperature Changes Based on SERS Technology
by Yusi Peng, Shuai Zhao, Masaki Tanemura, Yong Yang and Ming Liu
Biosensors 2025, 15(9), 558; https://doi.org/10.3390/bios15090558 - 22 Aug 2025
Viewed by 487
Abstract
Motivated by the rapid development of SERS technology, trace detection of various viruses in the sewage and body fluid environments and accurate positive and negative diagnosis of detection samples can be achieved. However, evaluating the environmental survival ability of viruses based on SERS [...] Read more.
Motivated by the rapid development of SERS technology, trace detection of various viruses in the sewage and body fluid environments and accurate positive and negative diagnosis of detection samples can be achieved. However, evaluating the environmental survival ability of viruses based on SERS technology remains an unexplored issue, but holds significant guiding significance for effective epidemic prevention and control as well as inactivation treatment. In this work, Au nanoarrays were fabricated on silicon substrates through a simple Ar ion sputtering route as ultra-sensitive SERS chips. With the synergistic contribution of the “lightning rod” effect and the enhanced coupling surface plasmon caused by the nanoarrays, the ultra-sensitive detection of SARS-CoV-2 S protein with a concentration of 1 pg/mL and SERS enhancement factor of 4.89 × 109 can be achieved. Exploration of the environmental survival ability of the SARS-CoV-2 virus indicates that the Raman activity of SARS-CoV-2 S protein exhibited higher temperature tolerance from 0 °C to 60 °C than SARS-CoV S protein, suggesting that the SARS-CoV-2 virus has less temperature influence from increasing air temperature than the SARS-CoV virus to a certain extent, which explains the seasonal recurrence pattern and regional transmission pattern of the novel coronavirus that are different from the SARS virus. Full article
(This article belongs to the Special Issue Nanomaterial-Based Biosensors for Point-of-Care Testing)
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17 pages, 1719 KB  
Article
A DNA Adsorption-Based Biosensor for Rapid Detection of Ratoon Stunting Disease in Sugarcane
by Moutoshi Chakraborty, Shamsul Arafin Bhuiyan, Simon Strachan, Muhammad J. A. Shiddiky, Nam-Trung Nguyen, Narshone Soda and Rebecca Ford
Biosensors 2025, 15(8), 518; https://doi.org/10.3390/bios15080518 - 8 Aug 2025
Viewed by 914
Abstract
Early and accurate detection of plant diseases is critical for ensuring global food security and agricultural resilience. Ratoon stunting disease (RSD), caused by the bacterium Leifsonia xyli subsp. xyli (Lxx), is among the most economically significant diseases of sugarcane worldwide. Its [...] Read more.
Early and accurate detection of plant diseases is critical for ensuring global food security and agricultural resilience. Ratoon stunting disease (RSD), caused by the bacterium Leifsonia xyli subsp. xyli (Lxx), is among the most economically significant diseases of sugarcane worldwide. Its cryptic nature—characterized by an absence of visible symptoms—renders timely diagnosis particularly difficult, contributing to substantial undetected yield losses across major sugar-producing regions. Here, we report the development of a potential-induced electrochemical (EC) nanobiosensor platform for the rapid, low-cost, and field-deployable detection of Lxx DNA directly from crude sugarcane sap. This method eliminates the need for conventional nucleic acid extraction and thermal cycling by integrating the following: (i) a boiling lysis-based DNA release from xylem sap; (ii) sequence-specific magnetic bead-based purification of Lxx DNA using immobilized capture probes; and (iii) label-free electrochemical detection using a potential-driven DNA adsorption sensing platform. The biosensor shows exceptional analytical performance, achieving a detection limit of 10 cells/µL with a broad dynamic range spanning from 105 to 1 copy/µL (r = 0.99) and high reproducibility (SD < 5%, n = 3). Field validation using genetically diverse sugarcane cultivars from an inoculated trial demonstrated a strong correlation between biosensor signals and known disease resistance ratings. Quantitative results from the EC biosensor also showed a robust correlation with qPCR data (r = 0.84, n = 10, p < 0.001), confirming diagnostic accuracy. This first-in-class EC nanobiosensor for RSD represents a major technological advance over existing methods by offering a cost-effective, equipment-free, and scalable solution suitable for on-site deployment by non-specialist users. Beyond sugarcane, the modular nature of this detection platform opens up opportunities for multiplexed detection of plant pathogens, making it a transformative tool for early disease surveillance, precision agriculture, and biosecurity monitoring. This work lays the foundation for the development of a universal point-of-care platform for managing plant and crop diseases, supporting sustainable agriculture and global food resilience in the face of climate and pathogen threats. Full article
(This article belongs to the Special Issue Nanomaterial-Based Biosensors for Point-of-Care Testing)
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