Special Issue "Systematic Genome Editing of the Genes on Zebrafish Chromosome by CRISPR/Cas9"

A special issue of Biology (ISSN 2079-7737). This special issue belongs to the section "Genetics and Genomics".

Deadline for manuscript submissions: 30 August 2021.

Special Issue Editors

Dr. De-Li Shi
E-Mail Website
Guest Editor
Laboratory of Developmental Biology, Institut de Biologie Paris-Seine, Sorbonne University, 9 quai Saint Bernard, 75252 PARIS CEDEX, France
Interests: genomic features; developmental biology; zebrafish; early embryo; genome editing; CRISPR/Cas9; Wnt signaling; gastrulation; myogenesis; morphogenesis
Dr. Bo Zhang
E-Mail Website
Guest Editor
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, Peking University Genome Editing Research Center, College of Life Sciences, Peking University, Beijing, 100871, China
Interests: zebrafish; genome editing; CRISPR/Cas system; organogenesis; heart regeneration; functional genomics; single-cell transcriptomics; disease modeling

Special Issue Information

Dear Colleagues,

The CRISPR/Cas9 system is a powerful tool in genome editing, with a wide range of potential applications. It has been used successfully in a variety of species, from invertebrates to mammals, making gene editing faster and more accessible than ever. This technology has produced an enormous effect on genetic research. Zebrafish has become particularly attractive for understanding regulatory gene functions and for modeling human diseases. The number of mutant and transgenic lines created by CRISPR/Cas9 is rapidly increasing in this commonly used animal model, which should help to illustrate the genotype–phenotype relationship in the future. It is expected that further improvements of CRISPR/Cas9-mediated conditional knockouts and transgenesis should strongly impact the systematic screening of gene functions and imaging-based cell lineage tracing during embryonic and post-embryonic development.

This Special Issue welcomes submissions of original research and review manuscripts focusing on the application of CRISPR/Cas9 technology and related molecular tools to genome editing in zebrafish, including but not limited to gene targeting and technical development or improvement. The aim is to provide an overview of the exciting advance and the strong potential for systematic analysis of gene functions in development and disease.

Dr. De-Li Shi
Dr. Bo Zhang

Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biology is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • Genetics
  • Knock-in
  • Zebrafish
  • Knock-out
  • Phenomics
  • Transgenesis
  • Chromosome
  • Disease model
  • Gene function
  • Regeneration
  • CRISPR/Cas9
  • Genome editing
  • Developmental biology
  • Lineage tracing

Published Papers

This special issue is now open for submission.
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