Sustainable Strategies for Natural Antioxidant Utilization

A special issue of Antioxidants (ISSN 2076-3921).

Deadline for manuscript submissions: 30 June 2026 | Viewed by 1294

Special Issue Editors


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Guest Editor
Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, 3603 Lemesos, Cyprus
Interests: food and natural product analysis; sample preparation; instrumental methods of analysis; bioactivity; chemometric data processing

Special Issue Information

Dear Colleagues,

Natural antioxidants are a rapidly evolving research field, driven by the increasing demand for sustainable and natural ingredients in the food, cosmetic, and pharmaceutical industries. This area is strongly influenced by the principles of green chemistry and the pursuit of innovative and sustainable technologies. Green chemistry emphasizes the minimization of environmental impact throughout the chemical process. For natural antioxidant utilization, this translates to the use of non-toxic and renewable solvents, low energy consumption, and reduced volumes of generated wastes. Sustainable and innovative techniques in the discovery and development of antioxidant-rich plant-based natural products and their analogs have been developed to address these drawbacks.

Due to the increasing consumer preference for natural products, stricter environmental regulations, and advancements in green chemistry, the sustainable extraction and valorization of natural antioxidants across diverse industries is becoming increasingly important. Therefore, natural antioxidants are being used in the food industry as food preservatives, functional foods, and dietary supplements, in skin and hair care products, and in the pharmaceutical industry as lead compounds or therapeutic agents.

This Special Issue invites cutting-edge research focused on sustainable and efficient methods for recovering valuable antioxidants from natural products. We aim to highlight advancements that contribute to a circular economy and promote environmentally friendly practices in the utilization of natural products.

Dr. Vlasios Goulas
Dr. Atalanti Christou
Guest Editors

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Keywords

  • extraction
  • antioxidants
  • polyphenols
  • phytochemicals
  • green chemistry
  • sustainable process
  • natural products
  • drug discovery
  • formulation
  • natural product analysis

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Published Papers (2 papers)

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Research

29 pages, 1891 KB  
Article
RSM- and ANN-Based Optimization of Bioactive Anthocyanin and Phenolic Compound Recovery from Rosa damascena Flowers Using Natural Deep Eutectic
by Georgia D. Ioannou, Atalanti Christou, Michaella Pieri, Panayiota Piri, George Botsaris and Vlasios Goulas
Antioxidants 2026, 15(6), 656; https://doi.org/10.3390/antiox15060656 - 22 May 2026
Viewed by 161
Abstract
The present study investigated the use of natural deep eutectic solvents (NaDESs) combined with ultrasound-assisted extraction (UAE) for the efficient recovery of anthocyanins and antioxidant phenolics from Damask rose (DR). A wide range of environmentally friendly solvents was screened, and choline chloride–propylene glycol [...] Read more.
The present study investigated the use of natural deep eutectic solvents (NaDESs) combined with ultrasound-assisted extraction (UAE) for the efficient recovery of anthocyanins and antioxidant phenolics from Damask rose (DR). A wide range of environmentally friendly solvents was screened, and choline chloride–propylene glycol (ChCl-PG) was identified as the most effective extraction medium. The extraction conditions were optimized using response surface methodology (RSM) and artificial neural network (ANN) models to maximize anthocyanin and phenolic contents, as well as antioxidant activity. Under the optimal parameters, the DR extracts exhibited relatively high levels of bioactive compounds, including total anthocyanin content of 5.2–5.3 mg cyanidin-3-glucoside equivalents g−1 sample, total phenolic content of 63.4–64.2 mg gallic acid equivalents g−1 sample, along with substantial antioxidant potential (DPPH: 68.2–68.8% inhibition, FRAP: 581.6–591.9 μmol Trolox equivalents g−1 sample). Chromatographic analysis of the optimum extract revealed cyanidin as the predominant anthocyanidin in DR, and its stability was further evaluated, revealing improved preservation under dark conditions at lower temperatures over a 15-day storage period. Moreover, the IC50 values confirmed antimicrobial effects against the tested foodborne pathogens. Furthermore, the inhibitory effect of the DR extract remained stable against S. aureus and S. cerevisiae throughout the storage period. Overall, the findings demonstrate that NaDES-UAE is a promising and sustainable approach for obtaining anthocyanin-rich DR extracts with antioxidant and antimicrobial potential. Full article
(This article belongs to the Special Issue Sustainable Strategies for Natural Antioxidant Utilization)
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28 pages, 1920 KB  
Article
Aspen Plus®-Validated CCD–RSM Optimisation of Pressurised Ethanol/Water Extraction for Sustainable Recovery of Antioxidant and Photoprotective Constituents from Inula salicina L.
by Marius Užupis, Michail Syrpas, Andrius Jaskūnas, Petras Rimantas Venskutonis and Vaida Kitrytė-Syrpa
Antioxidants 2026, 15(4), 466; https://doi.org/10.3390/antiox15040466 - 9 Apr 2026
Viewed by 642
Abstract
This study presents an integrated approach for producing antioxidant-rich polar fractions from Inula salicina L. via pressurised ethanol/water extraction (PLE-EtOH/H2O), optimised by coupling a central composite design and response surface methodology (CCD-RSM) with Aspen Plus® simulation. The effects of PLE [...] Read more.
This study presents an integrated approach for producing antioxidant-rich polar fractions from Inula salicina L. via pressurised ethanol/water extraction (PLE-EtOH/H2O), optimised by coupling a central composite design and response surface methodology (CCD-RSM) with Aspen Plus® simulation. The effects of PLE temperature, extraction time, and EtOH/H2O ratio for yield, total phenolic (TPC) and flavonoid (TFC) content, and Trolox equivalent antioxidant capacity (TEAC) measured in ABTS•+-scavenging, cupric ion reducing antioxidant (CUPRAC) and oxygen radical absorbance (ORAC) assays were assessed via a multi-response optimisation approach. Optimal conditions were set at 82 °C, 27 min, and 60% EtOH (v/v), yielding ~29 g extract per 100 g plant material, characterised by high TPC (227 mg GAE/g), TFC (34 mg QE/g), and TEAC values in the CUPRAC (1473 mg TE/g), ABTS (869 mg TE/g), and ORAC assays (1165 mg TE/g). The TPC and TEAC values of the post-extraction residue were >92% lower than those of unextracted I. salicina, confirming efficient recovery of the major portion of antioxidant-active constituents by PLE-EtOH/H2O. The high in vitro radical scavenging capacity, reducing power, and photoprotective potential (sun protection factor ~50 at 0.5 mg/mL) of the I. salicina extract are consistent with its phenolic-rich composition, with chlorogenic acid (~97 mg/g extract) and its derivatives being the major constituents. The validated Aspen Plus® model closely aligned with the CCD-RSM predictions, supporting process scale-up and energy feasibility and demonstrating an industry-relevant, green-solvent PLE process for producing higher value-added I. salicina fractions with potential applications in the food, pharmaceutical, nutraceutical, and cosmetic sectors. Full article
(This article belongs to the Special Issue Sustainable Strategies for Natural Antioxidant Utilization)
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