Antimicrobial and Antimicrobial Resistance in the Environment – 2nd Volume

A special issue of Antibiotics (ISSN 2079-6382).

Deadline for manuscript submissions: closed (30 April 2023) | Viewed by 8857

Special Issue Editors


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Guest Editor
1. Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisboa, Portugal
2. Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisboa, Portugal
3. Centre for Ecology, Evolution and Environmental Changes (cE3c) & Global Change and Sustainability Institute (CHANGE), Faculty of Sciences, University of Lisbon, Campo Grande, 1749-016 Lisboa, Portugal
Interests: one health; clinical bacteriology; biofilms; antimicrobial resistance; wildlife bacteriology; mycology; bacterial virulence; genomics; infections pathogenesis; food safety
Special Issues, Collections and Topics in MDPI journals
1. Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisboa, Portugal
2. Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisboa, Portugal
Interests: antimicrobial compounds; antimicrobial resistance; bacterial virulence; biofilms; veterinary medicine; infectious diseases
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Although the worldwide dissemination of multidrug-resistant strains represents a major concern for the safeguarding of One Health, the role of natural environments as reservoirs of antimicrobial-resistant strains and determinants as well as of antimicrobial residues has been overlooked. Monitoring environmental resistance is of major importance in this context, particularly in habitats that are highly affected by anthropogenic contamination. Indeed, antimicrobial compound residues and resistant strains originating from human activities and effluents, including agricultural and farming practices and recreational activities, can disseminate through the environment through wildlife vectors or ground waters, reaching settings in which no antimicrobials can be directly administered to animals or humans. Besides being related to the selective pressure imposed by the presence of antimicrobials residues, pesticides, heavy metals, and other chemicals, resistance dissemination in the environment can also be triggered by increased environmental temperature, which is one of the main consequences of climate change. In fact, the majority of bacterial species is able to easily adapt to an increase in environmental temperature, stimulating the dissemination of resistant determinants between the three areas of One Health –, i.e., humans, animals, and the environment.

As such, characterizing the presence of antimicrobials and antimicrobial resistance in the environment constitutes an important contribution to the establishment of effective surveillance and management programs that aim to unravel the evolution of resistance mechanisms and dissemination paths in natural environments as well as to evaluate potential consequences for human and animal health. This Special Issue constitutes a second volume that focuses on antimicrobials and antimicrobial resistance in the environment and aims to publish manuscripts that contribute to our understanding of the impact of antimicrobials and bacterial antimicrobial resistance in environmental health.

Dr. Manuela Oliveira
Dr. Eva Cunha
Guest Editors

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Keywords

  • agriculture
  • animal production
  • antimicrobial resistance
  • aquatic environment
  • environmental resistance
  • epidemiology
  • genomics
  • infections pathogenesis
  • One Health
  • wildlife

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Published Papers (3 papers)

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Research

14 pages, 785 KiB  
Article
ESBL-Positive Enterobacteriaceae from Dogs of Santiago and Boa Vista Islands, Cape Verde: A Public Health Concern
by Alice Matos, Eva Cunha, Lara Baptista, Luís Tavares and Manuela Oliveira
Antibiotics 2023, 12(3), 447; https://doi.org/10.3390/antibiotics12030447 - 23 Feb 2023
Cited by 4 | Viewed by 2318
Abstract
Antimicrobial resistance is a public health threat with an increasing expression in low- and middle-income countries such as Cape Verde. In this country, there is an overpopulation of dogs, which may facilitate the spread of resistant bacteria, including extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae. To [...] Read more.
Antimicrobial resistance is a public health threat with an increasing expression in low- and middle-income countries such as Cape Verde. In this country, there is an overpopulation of dogs, which may facilitate the spread of resistant bacteria, including extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae. To clarify the role of dogs as reservoirs for the dissemination of this bacterial group, 100 rectal swab samples were collected from confined (n = 50) and non-confined (n = 50) dogs in Santiago and Boa Vista Islands, Cape Verde. These were analyzed using conventional bacteriological techniques for the detection of ESBL-producing Enterobacteriaceae and characterization of their pathogenic and resistance profiles. Twenty-nine samples displayed ESBL-positive bacteria, from which 48 ESBL-producing isolates were obtained and mostly identified as Escherichia coli. Multiple antimicrobial resistance indexes ranged from 0.18 to 0.70 and half of the isolates were classified as multidrug-resistant. Isolates were capable of producing relevant virulence factors, including biofilm, showing virulence indexes between 0.29 and 0.71. As such, dogs in Cape Verde may act as reservoirs of resistant bacteria, including pathogenic and zoonotic species, representing a public health concern. Although further investigation is needed, this study proposes the periodical analysis of dogs’ fecal samples to monitor resistance dissemination in the country, in a One-Health perspective. Full article
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23 pages, 2181 KiB  
Article
Occurrence of Antimicrobial-Resistant Escherichia coli in Marine Mammals of the North and Baltic Seas: Sentinels for Human Health
by Stephanie Gross, Anja Müller, Diana Seinige, Peter Wohlsein, Manuela Oliveira, Dieter Steinhagen, Corinna Kehrenberg and Ursula Siebert
Antibiotics 2022, 11(9), 1248; https://doi.org/10.3390/antibiotics11091248 - 14 Sep 2022
Cited by 9 | Viewed by 3028
Abstract
Antimicrobial resistance is a global health threat that involves complex, opaque transmission processes in the environment. In particular, wildlife appears to function as a reservoir and vector for antimicrobial-resistant bacteria as well as resistance genes. In the present study, the occurrence of antimicrobial-resistant [...] Read more.
Antimicrobial resistance is a global health threat that involves complex, opaque transmission processes in the environment. In particular, wildlife appears to function as a reservoir and vector for antimicrobial-resistant bacteria as well as resistance genes. In the present study, the occurrence of antimicrobial-resistant Escherichia coli was determined in marine mammals and various fish species of the North and Baltic Seas. Rectal or faecal swabs were collected from 66 live-caught or stranded marine mammals and 40 fish specimens. The antimicrobial resistance phenotypes and genotypes of isolated E. coli were determined using disk diffusion tests and PCR assays. Furthermore, isolates were assigned to the four major phylogenetic groups of E. coli. Additionally, post mortem examinations were performed on 41 of the sampled marine mammals. The investigations revealed resistant E. coli in 39.4% of the marine mammal samples, while no resistant isolates were obtained from any of the fish samples. The obtained isolates most frequently exhibited resistance against aminoglycosides, followed by β-lactams. Of the isolates, 37.2% showed multidrug resistance. Harbour porpoises (Phocoena phocoena) mainly carried E. coli isolates belonging to the phylogenetic group B1, while seal isolates were most frequently assigned to group B2. Regarding antimicrobial resistance, no significant differences were seen between the two sampling areas or different health parameters, but multidrug-resistant isolates were more frequent in harbour porpoises than in the sampled seals. The presented results provide information on the distribution of antimicrobial-resistant bacteria in the North and Baltic Seas, and highlight the role of these resident marine mammal species as sentinels from a One Health perspective. Full article
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16 pages, 1727 KiB  
Article
Genetic Diversity and Virulence Profiling of Multi-Drug Resistant Escherichia coli of Human, Animal, and Environmental Origins
by Asfand Yar, Muhammad Adil Choudary, Abdul Rehman, Abid Hussain, Amina Elahi, Farooq ur Rehman, Ahmed Bilal Waqar, Abdulrahman Alshammari, Metab Alharbi, Muhammad Atif Nisar, Mohsin Khurshid and Zaman Khan
Antibiotics 2022, 11(8), 1061; https://doi.org/10.3390/antibiotics11081061 - 4 Aug 2022
Cited by 3 | Viewed by 2855
Abstract
Rapid urbanization has increased human-animal interaction and consequently enhanced the chances to acquire zoonotic diseases. The current investigation is focused to uncover the genetic diversity of multidrug-resistant E. coli strains between different ecologies (i.e., humans, livestock, and environment) at the molecular level by [...] Read more.
Rapid urbanization has increased human-animal interaction and consequently enhanced the chances to acquire zoonotic diseases. The current investigation is focused to uncover the genetic diversity of multidrug-resistant E. coli strains between different ecologies (i.e., humans, livestock, and environment) at the molecular level by employing antimicrobial resistance profiling, virulence genes profiling, and microbial typing approach using ERIC PCR. Based on multiple antibiotic resistance, overall, 19 antibiotic resistance patterns (R1–R19) were observed. Most of the strains (49/60) were detected to have the combinations of stx, eaeA, and hlyA genes and considered STEC/EPEC/EHEC. A total of 18 unique genetic profiles were identified based on ERIC-PCR fingerprints and most of the strains (13) belong to P1 whereas the least number of strains were showing profiles P7 and P8-P11 (one member each profile). The calculated values for Shannon index (H) for human, animal, and environment are 1.70, 1.82, and 1.78, respectively revealing the highest genetic diversity among the E. coli strains of animal origin. The study revealed that drug-resistant pathogenic E. coli strains could be transmitted bidirectionally among the environment, humans, and animals. Full article
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