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Antibiotics
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Aptamer-Based Biosensors for Antibiotic Detection
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Aptamer-Based Biosensors for Antibiotic Detection

A special issue of Antibiotics (ISSN 2079-6382).

Deadline for manuscript submissions: closed (31 August 2020) | Viewed by 14425

Special Issue Editor

Dr. Parvaneh Rahimi

E-Mail Website
Guest Editor
TU Bergakademie Freiberg, Institute of Electronic and Sensor Materials, Freiberg, Germany
Interests: biosensors; electrochemistry; nanomaterials; functionalization; nanocomposites
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The increase of antibiotic-resistant germs is an acute challenge for consumer health protection and veterinary medicine. The inappropriate and prophylactic use of antibiotics (especially in the field of animal care) is common and associated with the contamination of the environment with antibiotics and their metabolites. Therefore, simple, sensitive, robust, and rapid methods for the evaluation of antibiotics and their residues are needed for an on-site screening analysis. Currently, many studies confirm the application of biosensors as ideal alternatives to detect antibiotics in view of their superiority such as rapid detection, high selectivity, and in situ applications. Among different biosensors, aptamer-based biosensors (aptasensors) with different detection techniques are promising tools for a multitude of applications, especially for antibiotic detection.  

This Special Issue aims to highlight unique research and development efforts, identifying aptasensors for the detection of various antibiotics. Research papers, short communications, and reviews are all welcome. If you are interested in submitting a review, it would be helpful if you would discuss this with the Guest Editor before submission.

Dr. Parvaneh Rahimi
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antibiotics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • aptamers
  • biosensors
  • aptasensors
  • antibiotics
  • electrochemical
  • mass sensitive
  • optical

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Published Papers (3 papers)

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Research

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16 pages, 3213 KiB  
Article
Investigation of a Truncated Aptamer for Ofloxacin Detection Using a Rapid FRET-Based Apta-Assay
by Sondes Ben Aissa, Mohamed Mastouri, Gaëlle Catanante, Noureddine Raouafi and Jean Louis Marty
Antibiotics 2020, 9(12), 860; https://doi.org/10.3390/antibiotics9120860 - 3 Dec 2020
Cited by 9 | Viewed by 3995
Abstract
In this work, we describe the use of a new truncated aptamer for the determination of ofloxacin (OFL), being a principal quinolone commonly used in both human and animal healthcare. Since the affinity of a 72-mer ssDNA sequence has been previously described without [...] Read more.
In this work, we describe the use of a new truncated aptamer for the determination of ofloxacin (OFL), being a principal quinolone commonly used in both human and animal healthcare. Since the affinity of a 72-mer ssDNA sequence has been previously described without further investigations, this paper demonstrates the first computational prediction of the binding motif between this aptamer and OFL through in silico molecular docking studies. Besides, we suggest the application of the characterized recognition mechanism in a simple FRET (Förster Resonance Energy Transfer) pattern for the rapid aptasensing of the quinolone of interest. Accordingly, our approach harnesses the fluorescence quenching of the fluorescein-tagged aptamer (FAM-APT) induced by its partial hybridization to a tetramethyl rhodamine-labelled complementary ssDNA (TAMRA-cDNA). In such a structure, dye labels brought into close proximity act as a FRET pair. Upon ofloxacin addition, an affinity competition occurs to form a more stable FAM-APT/OFL complex, thus unquenching the FAM-APT signal. Interestingly, the recovered fluorescence intensity was found to correlate well with the antibiotic’s concentrations in the range of 0.2–200 μM in HEPES buffer, with a linear response that ranged between 0.2 and 20 μM. The rapid apta-assay achieved limits of detection and quantification of 0.12 and 0.40 μM, respectively. The truncated aptamer has also shown an improved specificity toward OFL than other quinolones, compared to the original full-length aptamer described in previous works. Finally, the practical application of the developed apta-assay was successfully confirmed to detect OFL quinolone in spiked milk samples, with satisfactory recoveries ranging between 97.4% and 111.4%. Full article
(This article belongs to the Special Issue Aptamer-Based Biosensors for Antibiotic Detection)
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16 pages, 14222 KiB  
Article
Aptamer-Based Detection of Ampicillin in Urine Samples
by Matthew D. Simmons, Lisa M. Miller, Malin O. Sundström and Steven Johnson
Antibiotics 2020, 9(10), 655; https://doi.org/10.3390/antibiotics9100655 - 29 Sep 2020
Cited by 14 | Viewed by 4538
Abstract
The misuse of antibiotics in health care has led to increasing levels of drug resistant infections (DRI’s) occurring in the general population. Most technologies developed for the detection of DRI’s typically focus on phenotyping or genotyping bacterial resistance rather than on the underlying [...] Read more.
The misuse of antibiotics in health care has led to increasing levels of drug resistant infections (DRI’s) occurring in the general population. Most technologies developed for the detection of DRI’s typically focus on phenotyping or genotyping bacterial resistance rather than on the underlying cause and spread of DRI’s; namely the misuse of antibiotics. An aptameric based assay has been developed for the monitoring of ampicillin in urine samples, for use in determining optimal antibiotic dosage and monitoring patient compliance with treatment. The fluorescently labelled aptamers were shown to perform optimally at pH 7, ideal for buffered clinical urine samples, with limits of detection as low as 20.6 nM, allowing for determination of ampicillin in urine in the clinically relevant range of concentrations (100 nM to 100 µM). As the assay requires incubation for only 1 h with a small sample volume, 50 to 150 µL, the test would fit within current healthcare pathways, simplifying the adoption of the technology. Full article
(This article belongs to the Special Issue Aptamer-Based Biosensors for Antibiotic Detection)
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Review

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18 pages, 1719 KiB  
Review
Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics
by Yunxia Luan, Nan Wang, Cheng Li, Xiaojun Guo and Anxiang Lu
Antibiotics 2020, 9(11), 787; https://doi.org/10.3390/antibiotics9110787 - 7 Nov 2020
Cited by 50 | Viewed by 4992
Abstract
Antibiotic abuse is becoming increasingly serious and the potential for harm to human health and the environment has aroused widespread social concern. Aminoglycoside antibiotics (AGs) are broad-spectrum antibiotics that have been widely used in clinical and animal medicine. Consequently, their residues are commonly [...] Read more.
Antibiotic abuse is becoming increasingly serious and the potential for harm to human health and the environment has aroused widespread social concern. Aminoglycoside antibiotics (AGs) are broad-spectrum antibiotics that have been widely used in clinical and animal medicine. Consequently, their residues are commonly found in animal-derived food items and the environment. A simple, rapid, and sensitive detection method for on-site screening and detection of AGs is urgently required. In recent years, with the development of molecular detection technology, nucleic acid aptamers have been successfully used as recognition molecules for the identification and detection of AGs in food and the environment. These aptamers have high affinities, selectivities, and specificities, are inexpensive, and can be produced with small batch-to-batch differences. This paper reviews the applications of aptamers for AG detection in colorimetric, fluorescent, chemiluminescent, surface plasmon resonance, and electrochemical sensors for the analysis in food and environmental samples. This study provides useful references for future research. Full article
(This article belongs to the Special Issue Aptamer-Based Biosensors for Antibiotic Detection)
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