Genome Editing of Polyploid Crops

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Crop Breeding and Genetics".

Deadline for manuscript submissions: closed (31 May 2023) | Viewed by 4149

Special Issue Editors


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Guest Editor
Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 5403 1st Avenue South, Lethbridge, AB T1J 4B1, Canada
Interests: gene editing; RNAi; cereal biotechnology; plant reproduction

E-Mail Website
Guest Editor
Agriculture and Agri-Food Canada, Kentville Research and Development Centre, 32 Main St., Kentville, NS B4N 1J5, Canada
Interests: fungal disease management; plant-microbe interaction; plant pathology; plant–fungal endophyte interaction; pathogen identification using molecular biology techniques
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Special Issue Information

Dear Colleagues,

Population growth together with changing climate are driving the need for rapid crop improvement. To assist conventional breeding with this monumental challenge, technological innovation is required. Induced mutagenesis has helped greatly over the past several decades, but the technology is messy and time consuming. Gene editing, however, is one such technology that offers great potential for rapid crop improvement because of its precision and ease. A challenge for many crops is their polyploidy nature. Function redundancy because of multiple gene copies can mask genetic variation. With gene editing, however, we now have the capability of manipulating every gene in the genome, including those that are multi-copy and redundant. This special issue of Agronomy seeks to highlight the potential value of gene editing in polyploidy crops. We welcome contributions that will address the use of gene editing in polyploidy crops. Topics of interest include, but are not limited to 1) increase genetic variation, 2) remove deleterious or unwanted genes, 3) increase recombination, 4) induce genome fractionation, 5) facilitate gene introduction from wild relatives, 6) produce agronomically important traits.

In this Special Issue, we welcome articles (original research papers, perspectives, hypotheses, opinions, reviews and methods) focusing on genome editing in polyploidy crops.

We look forward to your contribution.

Dr. John Laurie
Dr. Shawkat Ali
Guest Editors

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Keywords

  • polyploid
  • genetic variation
  • knock-out
  • recombination
  • deleterious allele
  • genome fractionation
  • biotic and abiotic stress

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Published Papers (1 paper)

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Research

14 pages, 2022 KiB  
Article
New Flowering and Architecture Traits Mediated by Multiplex CRISPR-Cas9 Gene Editing in Hexaploid Camelina sativa
by Yannick Bellec, Anouchka Guyon-Debast, Tracy François, Lionel Gissot, Eric Biot, Fabien Nogué, Jean-Denis Faure and Mark Tepfer
Agronomy 2022, 12(8), 1873; https://doi.org/10.3390/agronomy12081873 - 9 Aug 2022
Cited by 9 | Viewed by 3162
Abstract
Adapting plants to sustainable cropping systems is a major challenge for facing climate change and promoting agroecological transition. Camelina sativa is an emerging oilseed crop species with climate-resilient properties that could be used in double-cropping systems, in particular as a summer catch crop. [...] Read more.
Adapting plants to sustainable cropping systems is a major challenge for facing climate change and promoting agroecological transition. Camelina sativa is an emerging oilseed crop species with climate-resilient properties that could be used in double-cropping systems, in particular as a summer catch crop. The availability of early-flowering camelina is essential in such cropping systems to allow full completion of the growth cycle during summer. Targeted induced gene variation (TIGV) was used in camelina on several flowering repressor genes identified in Arabidopsis to obtain early-flowering lines. Multiplex editing of 15 target genes representing FLOWERING LOCUS C, SHORT VEGETATIVE PHASE, LIKE HETEROCHROMATIN PROTEIN 1, TERMINAL FLOWER 1 and EARLY FLOWERING LOCUS 3 induced combinatorial mutations that were screened for early-flowering phenotypes. Certain mutants showing a stable early-flowering trait after five generations also presented additional phenotypes: determinate flowering, shorter stature and/or basal branching. Different combinations of mutations had a positive or negative impact on yield. This work demonstrates that efficient multiplex CRISPR is achievable in hexaploid plants like camelina, providing valuable genetic diversity for better selecting lines adapted to new cropping systems. Full article
(This article belongs to the Special Issue Genome Editing of Polyploid Crops)
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