Biotechnological Applications in Horticulture and Vegetable Crops

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Horticultural and Floricultural Crops".

Deadline for manuscript submissions: closed (15 October 2021) | Viewed by 5346

Special Issue Editors


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Guest Editor
CREA Research Centre for Vegetable and Ornamental crops, Via Salaria 1, 63077 Monsampolo del Tronto (AP), Italy
Interests: agricultural plant science; horticulture; plant breeding and genetics; genetic transformation; in vitro tissue culture; germplasm conservation; genetic diversity
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
CREA, Research Centre for Vegetable and Ornamental Crops, Via Salaria 1, 63030 Monsampolo del Tronto, AP, Italy
Interests: molecular biology; in vitro culture; gene expression; genetic transformation; novel breeding techniques (cisgenesis and gene editing); genetic resources; plant biotic stress resistance; horticultural plant breeding
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
CREA Research Centre for Vegetable and Ornamental Crops, Corso degli Inglesi 508, 18038 Sanremo (Imperia), Italy
Interests: floriculture; propagation; plant biotechnology; ex situ conservation; genetic improvement

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Guest Editor
CREA Research Centre for Vegetable and Ornamental Crops, Via Salaria 1, 63077 Monsampolo del Tronto (AP), Italy
Interests: molecular characterization; marker-assisted selection; plant transformation; micropropagation; tomato; strawberry; grapevine; cisgenesis; genome editing; CRISPR/Cas

Special Issue Information

Dear Colleagues,

Horticulture is an important and integral part of the economy that contributes significantly to the total agricultural production and covers several crops such as vegetables, fruits, flowers, tuber crops, spices, medicinal, and aromatic plants. The importance of horticulture can be substantiated by its benefits, such as providing variety, color, and flavor to the foods we eat; enriching our lives with many aesthetically appealing products; and helping to sustain a healthy environment. Classical breeding techniques are insufficient for the desired genetic improvement. Plant breeders in the past several decades have used inter-specific sexual hybridization to transfer genes between species. Sexual incompatibility barriers severely limit the possibilities for gene transfer between species. Genetic engineering can be used to add specific characteristics to existing cultivars. Modern biotechnological applications such as plant tissue culture (conservation and propagation of valuable, rare, and endangered plants), cell culture and tissue engineering (mass production of bioactive constituents via callus or suspension cultures), and genetic engineering (genome-editing techniques, cisgenesis/intragenesis) plays significant roles in contributing to the production and supply of improved quality. This Special Issue will offer an updated overview of biotechnological protocols in horticulture and will provide examples of successes that could be realized in agricultural biotechnology. The contributions will also introduce the scientific community to some of the exciting works available to improve quality in consideration of sustainability, for a future that includes more nutritious and healthy fruits and vegetables, as well as more fragrant and colorful flowers.

Dr. Nadia Ficcadenti
Dr. Sara Sestili
Dr. Annalisa Giovannini
Dr. Daniela Palma
Guest Editors

Manuscript Submission Information

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Keywords

  • horticultural crops
  • floriculture
  • tissue culture
  • genetic diversity
  • molecular breeding
  • plant breeding and genetics
  • plant transformation
  • genome editing
  • cisgenesis
  • root-targeted biotechnology
  • molecular biotechnology of natural products

Published Papers (1 paper)

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Research

20 pages, 7707 KiB  
Article
Ovaries of Chrysanthemum Irradiated with High-Energy Photons and High-Energy Electrons Can Regenerate Plants with Novel Traits
by Natalia Miler, Iwona Jedrzejczyk, Seweryn Jakubowski and Janusz Winiecki
Agronomy 2021, 11(6), 1111; https://doi.org/10.3390/agronomy11061111 - 29 May 2021
Cited by 11 | Viewed by 3794
Abstract
Classical mutation breeding using physical factors is a common breeding method for ornamental crops. The aim of our study was to examine the utility of ovaries excised from irradiated inflorescences of Chrysanthemum × morifolium (Ramat.) as explants for breeding purposes. We studied the [...] Read more.
Classical mutation breeding using physical factors is a common breeding method for ornamental crops. The aim of our study was to examine the utility of ovaries excised from irradiated inflorescences of Chrysanthemum × morifolium (Ramat.) as explants for breeding purposes. We studied the in vitro regeneration capacity of the ovaries of two chrysanthemum cultivars: ‘Profesor Jerzy’ and ‘Karolina’ preceded by irradiation with high-energy photons (total dose 5, 10 and 15 Gy) and high-energy electrons (total dose 10 Gy). Growth and inflorescence parameters of greenhouse acclimatized regenerants were recorded, and ploidy level was estimated with flow cytometry. The strong impact of genotype on regeneration efficiency was recorded—cultivar ‘Karolina’ produced only 7 viable shoots, while ‘Profesor Jerzy’ produced totally 428 shoots. With an increase of irradiation dose, the regeneration decreased, the least responsive were explants irradiated with 15 Gy high-energy photons and 10 Gy high-energy electrons. Regenerants of ‘Profesor Jerzy’ obtained from these explants possessed shorter stem and flowered later. The highest number of stable, color and shape inflorescence variations were obtained from explants treated with 10 Gy high-energy photons. Variations of inflorescences were predominantly changes of shape—from full to semi-full. New color phenotypes were dark yellow, light yellow and pinkish, among them only the dark yellow phenotype remained stable during second year cultivation. None of the regenerants were haploid. The application of ovaries irradiated within the whole inflorescence of chrysanthemum can be successfully applied in the breeding programs, provided the mother cultivar regenerate in vitro efficiently. Full article
(This article belongs to the Special Issue Biotechnological Applications in Horticulture and Vegetable Crops)
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