Advances in Tomato Breeding: Improving Yield and Quality

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Horticultural and Floricultural Crops".

Deadline for manuscript submissions: 30 June 2026 | Viewed by 1090

Special Issue Editors


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Guest Editor
College of Horticulture, Northwest A&F University, Xianyang 712100, China
Interests: tomato resources; tomato breeding; tomato quality; tomato stress

E-Mail Website
Guest Editor
College of Agriculture, Guizhou University, Guizhou 550025, China
Interests: tomato breeding; tomato quality; tomato virus; tomato stress

Special Issue Information

Dear Colleagues,

This Special Issue, entitled "Advances in Tomato Breeding: Improving Yield and Quality", addresses the critical challenge of enhancing tomato production to meet global food demand. The background lies in the tomato's status as a prime horticultural crop, where decades of breeding have often prioritized yield improvements at the expense of key quality traits such as flavor and nutritional content. This Special Issue seeks to bridge that gap by highlighting integrative approaches that simultaneously advance both yield and multifaceted quality attributes.

The scope of this Special Issue spans genetics, physiology, stress tolerance, and management practices, featuring cutting-edge research on advanced breeding techniques—including CRISPR-Cas9 gene editing and QTL mapping—physiological studies on source–sink relationships, and sustainable strategies to mitigate biotic and abiotic stresses such as viruses, fungi, bacteria, and pests. We welcome the submission of original research and review articles that offer novel genetic insights, elucidate underlying physiological mechanisms, or introduce innovative cultivation technologies aimed at developing high-yielding tomato varieties without compromising quality.

Prof. Dr. Yan Liang
Dr. Yunzhou Li
Guest Editors

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Keywords

  • tomato
  • biotic or abiotic stress
  • breeding
  • quality
  • resources

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Published Papers (1 paper)

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Research

20 pages, 4085 KB  
Article
The SlPCL1–SlSUMO1 Complex Defines a SlPCL1–SlNPF4.6 Module Governs Cold Tolerance in Tomato
by Yafei Qin, Jianfang Qiu, Mengyu Li, Mei Wang, Daodao Tang, Lei Ni, Chunyu Shang, Lang Wu, Yu Pan, Jinhua Li and Xingguo Zhang
Agronomy 2026, 16(4), 420; https://doi.org/10.3390/agronomy16040420 - 9 Feb 2026
Viewed by 666
Abstract
The circadian clock genes in tomato are key regulators of cold stress adaptation. However, the low-temperature regulatory mechanism of the circadian clock gene SlPCL1 remains unclear. In this study, we evaluated the role of SlPCL1 in cold tolerance through low-temperature treatment of transgenic [...] Read more.
The circadian clock genes in tomato are key regulators of cold stress adaptation. However, the low-temperature regulatory mechanism of the circadian clock gene SlPCL1 remains unclear. In this study, we evaluated the role of SlPCL1 in cold tolerance through low-temperature treatment of transgenic plants. Downstream target genes were identified using RNA-seq, RT-qPCR, yeast-one-hybrid (Y1H), dual-luciferase assays, and electrophoretic mobility shift assay (EMSA), while interacting proteins were characterized using yeast-two-hybrid (Y2H), luciferase complementation imaging (LCI), and pull-down assays, thereby elucidating the molecular mechanism underlying SlPCL1-mediated low-temperature regulation. We identified SlPCL1 as a nuclear-localized circadian clock gene with transcriptional repressor activity. Overexpression of SlPCL1 resulted in a cold-sensitive phenotype, whereas virus-induced gene silencing (VIGS)-mediated silencing of SlPCL1 enhanced cold tolerance. SlNPF4.6 functions as an abscisic acid (ABA) transporter involved in ABA transport. RNA-seq and RT-qPCR identified the ABA transporter SlNPF4.6 as a downstream target. Functional assays confirmed that SlPCL1 binds to the MYB element in the SlNPF4.6 promoter to repress its expression. Meanwhile, VIGS-mediated silencing of SlNPF4.6 decreased cold tolerance. Furthermore, the expression levels of the ABA receptor SlPYLs in the silenced lines were significantly reduced, confirming the decrease in intracellular ABA content. SlSUMO1, a ubiquitin-like protein, can influence gene transcription through noncovalent interactions. In addition, SlSUMO1 was found to interact with the SlPCL1 protein, attenuating SlPCL1 transcriptional repression activity. Together, these findings establish an SlSUMO1-mediated fine control mechanism of the SlPCL1-SlNPF4.6 regulatory module. This integration of circadian clock regulation uncovers new molecular mechanisms of cold tolerance and supports the development of cold-resistant breeding materials. Full article
(This article belongs to the Special Issue Advances in Tomato Breeding: Improving Yield and Quality)
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