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Cytomorphological Characterization of Individual Metastatic Tumor Cells from Gastrointestinal Cancer Patient Lymph Nodes with Imaging Flow Cytometry

1
Future Industries Institute and ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of South Australia, Adelaide 5095, Australia
2
Faculty of Health and Medical Sciences, University of Adelaide, Adelaide 5000, Australia
3
Department of Surgical Pathology, SA Pathology, Centre for Cancer Biology, University of South Australia, Adelaide 5000, Australia
*
Author to whom correspondence should be addressed.
Gastrointest. Disord. 2019, 1(4), 372-384; https://doi.org/10.3390/gidisord1040030
Received: 16 July 2019 / Revised: 17 September 2019 / Accepted: 19 September 2019 / Published: 26 September 2019
The presence or absence of tumor cells within patient lymph nodes is an important prognostic indicator in a number of cancer types and an essential element of the staging process. However, patients with the same pathological stage will not necessarily have the same outcome. Therefore, additional factors may aid in identifying patients at a greater risk of developing metastasis. In this proof of principle study, initially, spiked tumor cells in rat lymph nodes were used to mimic a node with a small cancer deposit. Next, human lymph nodes were obtained from cancer patients for morphological characterization. Nodes were dissociated with a manual tissue homogenizer and stained with fluorescent antibodies against CD45 and Pan-Cytokeratin and then imaging flow cytometry (AMNIS ImageStreamX Mark II) was performed. We show here that imaging flow cytometry can be used for the detection and characterization of small numbers of cancer cells in lymph nodes and we also demonstrate the phenotypical and morphological characterization of cancer cells in gastrointestinal cancer patient lymph nodes. When used in addition to conventional histological techniques, this high throughput detection of tumor cells in lymph nodes may offer additional information assisting in the staging process with therapeutic and prognostic applications. View Full-Text
Keywords: imaging flow cytometry; lymph node; metastasis; cellular morphology; cancer imaging flow cytometry; lymph node; metastasis; cellular morphology; cancer
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Winter, M.; Gibson, R.; Ruszkiewicz, A.; Thierry, B. Cytomorphological Characterization of Individual Metastatic Tumor Cells from Gastrointestinal Cancer Patient Lymph Nodes with Imaging Flow Cytometry. Gastrointest. Disord. 2019, 1, 372-384.

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