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7 pages, 213 KB

Open AccessProceeding Paper

Antioxidant Potential of Ergosterol–Phospholipid Liposomes with Thymus serpyllum Extract

by Dragana Dekanski, Andrea Pirković, Diana Lupulović, Predrag Petrović and Aleksandra A. Jovanović

Proceedings 2025, 119(1), 1; https://doi.org/10.3390/proceedings2025119001 - 15 May 2025

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The antioxidant activity of ergosterol–phospholipid liposomes with chemically characterized, encapsulated wild thyme (Thymus serpyllum) extract was examined. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity and ferric ion-reducing potential of the extract-loaded liposomes with different proportions of ergosterol were investigated. [...] Read more.

The antioxidant activity of ergosterol–phospholipid liposomes with chemically characterized, encapsulated wild thyme (Thymus serpyllum) extract was examined. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity and ferric ion-reducing potential of the extract-loaded liposomes with different proportions of ergosterol were investigated. The neutralization of DPPH radicals for the samples with 10 and 20 mol% of ergosterol was 56.3 ± 2.2% and 53.1 ± 3.5%, respectively. The elimination of ABTS radicals was significantly higher and amounted to 95.3 ± 2.6% (10 mol% of ergosterol) and 98.2 ± 1.7% (20 mol% of ergosterol). The Fe³⁺-reducing potential of the liposomes was 0.14 ± 0.01 and 0.15 ± 0.03 mmol FeSO₄/L. Therefore, there was no significant difference between the antioxidant capacity of the liposomes with various amounts of ergosterol. The shown antioxidant potential highlights the employment of prepared liposomes with ergosterol and T. serpyllum extract active compounds in functional foods, pharmaceutics, or cosmetics. Full article

(This article belongs to the Proceedings of The 2nd International Electronic Conference on Antioxidants)

7 pages, 215 KB

Open AccessProceeding Paper

Radical Scavenging and Ion-Reducing Capacity of Fumaria officinalis Extracts Obtained by Traditional and Assisted Extraction Techniques

by Rabiea Ashowen Ahmoda, Andrea Pirković, Violeta Milutinović, Dragana Dekanski, Aleksandar Marinković and Aleksandra A. Jovanović

Proceedings 2025, 119(1), 2; https://doi.org/10.3390/proceedings2025119002 - 15 May 2025

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Abstract

The present research aimed to extract antioxidants from the fumitory aerial part in the flowering stage (containing leaves, stems, and flowers) by performing traditional and novel extraction procedures (maceration, ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE)). The fumitory macerate showed significantly lower ABTS [...] Read more.

The present research aimed to extract antioxidants from the fumitory aerial part in the flowering stage (containing leaves, stems, and flowers) by performing traditional and novel extraction procedures (maceration, ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE)). The fumitory macerate showed significantly lower ABTS radical scavenging activity, expressed as a higher IC₅₀ value (the concentration of extract required to neutralize 50% of radicals, 11.4 ± 0.1 mg/mL), in comparison to the other two extracts, whose IC₅₀ values varied in a narrow range (8.6–9.5 mg/mL). In the DPPH assay, the trend was different: MAE (11.4 ± 0.3 mg/mL) ≥ UAE (12.0 ± 0.8 mg/mL) ≥ macerate (12.8 ± 0.1 mg/mL). In the CUPRAC assay, the UAE and MAE extracts (17.84 ± 0.85 and 18.05 ± 0.71 µmol Trolox equivalents (TE)/g, respectively) showed significantly higher antioxidant activity compared to the macerate (16.43 ± 0.45 µmol TE/g). Regarding the results of the FRAP method, there was no statistically significant difference in ferric ion reduction between the macerate, UAE, and MAE extracts (3.00–3.27 µmol Fe²⁺/g). However, the extract prepared using MAE provided the highest antioxidant potential, as shown in all four tests used. Due to demonstrated extracts’ antioxidant properties, additional research could address additional biological effects or the creation of delivery systems or encapsulates for the controlled delivery of fumitory bioactives. Full article

(This article belongs to the Proceedings of The 2nd International Electronic Conference on Antioxidants)

6 pages, 352 KB

Open AccessProceeding Paper

Bee Product Royal Jelly Reduces Oxidative Stress in Healthy MRC-5 Cells and Upregulates GSTP1 Expression

by Milena Jovanović and Dragana Šeklić

Proceedings 2025, 119(1), 3; https://doi.org/10.3390/proceedings2025119003 - 27 May 2025

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Abstract

Redox homeostasis in the human body is strictly regulated by reducing molecules, such as glutathione, as well as various antioxidant enzymes. Examination of the antioxidant effects of natural products is necessary in order to prevent and treat various pathological conditions considering the correlation [...] Read more.

Redox homeostasis in the human body is strictly regulated by reducing molecules, such as glutathione, as well as various antioxidant enzymes. Examination of the antioxidant effects of natural products is necessary in order to prevent and treat various pathological conditions considering the correlation of their occurrence with oxidative stress damage. RJ has been identified as a very potent regulator of many metabolic processes and is considered as a medicinal agent that can cope with the oxidative stress. The present study evaluated the RJ’s ability to scavenge superoxide anion radicals O₂^∙− and modulate the expression of GSTP1 marker in healthy lung fibroblasts (MRC-5 cell line) after 24 h. Assessment was performed with the NBT test and quantitative real-time polymerase chain reaction (qPCR). Our results show that RJ successfully reduced the O₂^∙− concentration for ~12% and upregulated GSTP1 gene expression (1.75 fold-change) whose protein product is responsible for catalyzation of glutathione (GSH) binding to oxidative stress metabolites and their further neutralization in cells. We found that RJ has an important protective effect against oxidative damage of healthy human cells and these properties could be used to explore new resources for pharmacological treatments, as well as to improve application of natural medicine for maintaining human health. Full article

(This article belongs to the Proceedings of The 2nd International Electronic Conference on Antioxidants)

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7 pages, 209 KB

Open AccessProceeding Paper

Stability of Cotinus coggygria Scop. Extract-Loaded Liposomes: The Impact of Storage on Physical and Antioxidant Properties

by Aleksandra A. Jovanović, Amjed Karkad, Andrea Pirković, Dragana Dekanski, Diana Lupulović, Miroslav Novaković and Aleksandar Marinković

Proceedings 2025, 119(1), 4; https://doi.org/10.3390/proceedings2025119004 - 28 May 2025

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Abstract

The stability of Cotinus coggygria extract-loaded liposomes (non-treated and UV-irradiated) was determined after 60 days through an investigation of the impact of storage on liposomal physical and antioxidant properties. The liposome size varied in a narrow range for 60 days; PDI was 0.273–0.313 [...] Read more.

The stability of Cotinus coggygria extract-loaded liposomes (non-treated and UV-irradiated) was determined after 60 days through an investigation of the impact of storage on liposomal physical and antioxidant properties. The liposome size varied in a narrow range for 60 days; PDI was 0.273–0.313 (non-treated) and 0.829–0.911 (UV-irradiated). The zeta potential ranged from −28.2 to −29.6 mV (non-treated) and from −21.5 to −22.0 mV (UV-irradiated). The obtained liposomes with the extract neutralized 81.9% of free DPPH radicals before UV irradiation and 80.9% after irradiation. In the ABTS assay, UV irradiation also significantly reduced the antioxidant capacity, from 12.02 to 10.55 µmol Trolox equivalent (TE)/mL. The ABTS and DPPH radical scavenging activity of the UV-irradiated liposomes significantly decreased after the 60-day storage (8.93 µmol TE/mL and 75.4%, respectively), whereas in the non-treated sample, the mentioned drop in antioxidant capacity was not noticed. Liposomal formulations of C. coggygria extract can exhibit significant potential for further development as a functional food or dermo-cosmetic ingredient. Full article

(This article belongs to the Proceedings of The 2nd International Electronic Conference on Antioxidants)

6 pages, 349 KB

Open AccessProceeding Paper

From Waste to Value: Phenolic Content and Antioxidant Potential in Cistus ladanifer Residues via Solid–Liquid and Subcritical Water Extraction

by Filipe Fernandes, Cristina Delerue-Matos and Clara Grosso

Proceedings 2025, 119(1), 5; https://doi.org/10.3390/proceedings2025119005 - 26 Jun 2025

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Abstract

The aim of this work was to extract phenolic compounds (PCs) from Cistus ladanifer L. post-distillation residues using two different methods (solid–liquid extraction (SLE) and subcritical water extraction (SWE)) and to compare the extracts’ total phenolic content (TPC) and antioxidant activity (AA) by [...] Read more.

The aim of this work was to extract phenolic compounds (PCs) from Cistus ladanifer L. post-distillation residues using two different methods (solid–liquid extraction (SLE) and subcritical water extraction (SWE)) and to compare the extracts’ total phenolic content (TPC) and antioxidant activity (AA) by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH^•) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS^•+) scavenging activities, as well as by the ferric-reducing antioxidant power (FRAP) assay. SWE extraction displayed a higher TPC value (increased from 146.53 ± 11.68 to 276.37 ± 20.59 mg gallic acid equivalents (GAEs)/g extract dry weight (dw)) and higher AA in the DPPH^• (increased from 334.27 ± 36.06 to 532.17 ± 66.38 mg Trolox equivalents (TEs)/g extract dw), ABTS^•+ (increased from 438.07 ± 77.22 to 594.08 ± 33.57 mg TEs/g extract dw), and FRAP (increased from 10.91 ± 2.03 to 170.26 ± 25.36 mg ascorbic acid equivalents (AAEs)/g extract dw) assays. These results demonstrate the importance of the extraction method in PC extraction and the antioxidant power of the extracts produced. These results provide critical insights into the potential application of C. ladanifer post-distillation residues and the production of polyphenol rich extracts that might be useful in the food, cosmetic, and pharmaceutical sectors. Full article

(This article belongs to the Proceedings of The 2nd International Electronic Conference on Antioxidants)

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7 pages, 193 KB

Open AccessProceeding Paper

Oxidative Stress, Inflammation, and Obesity: Insights into Mechanism and Therapeutic Targets

by Bhagyashri Sandip Patil, Javesh Kashinath Patil, Hemangi Somnath Chaudhari and Bhagyashri Sunil Patil

Proceedings 2025, 119(1), 6; https://doi.org/10.3390/proceedings2025119006 - 27 Jun 2025

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Abstract

Due to being correlated with metabolic syndrome, diabetes mellitus, cardiovascular disease, fatty liver disease, and cancer, obesity is a global health issue that predisposes those affected to morbidity and mortality. Obesity can be defined as an excessive amount of fat accretion in the [...] Read more.

Due to being correlated with metabolic syndrome, diabetes mellitus, cardiovascular disease, fatty liver disease, and cancer, obesity is a global health issue that predisposes those affected to morbidity and mortality. Obesity can be defined as an excessive amount of fat accretion in the body. According to current research, visceral adipose tissue performs a critical function as an active endocrine organ due to its function in releasing adipokines that facilitate complex physiological events. These adipokines exacerbate both low-grade inflammation and oxidative stress (OS), two key constituents of obesity-related comorbidities. This review summarizes the most recent data on the relationship between inflammation, OS, and diseases linked to obesity, focusing on how OS overexpression causes cellular damage by weakening antioxidant mechanisms. To understand the mechanisms by which OS is related to comorbidities, we assess a wide range of models, including animal models, biochemical analysis, and clinical research. The most important discoveries are that heightened OS exacerbates inflammation and cellular damage by increasing the formation of ROS and weakening antioxidant defenses. Increased lipid peroxidation and oxidative damage in adipose tissue associated with insulin resistance and metabolic dysfunction have been identified through data from research conducted on KKAy mice, a model of diabetes obesity. Adipokines, like adiponectin, have been shown to have protective functions against inflammation and OS. Thereby, some of these candidates may become promising therapeutic targets. Understanding the mechanism of these systems is a must for developing therapies to decrease OS, restore antioxidant balance, and reprogram inflammatory pathways. Such tactics may further augment clinical outcomes and reduce the occurrence of obesity-associated diseases in global populations. Unlike previous reviews, this work bridges basic mechanisms and therapeutic implications, with a unique emphasis on translational barriers and future clinical directions. Full article

(This article belongs to the Proceedings of The 2nd International Electronic Conference on Antioxidants)

5 pages, 194 KB

Open AccessProceeding Paper

Antioxidant Capacity of Colombian Tropical Fruits with Dietary Potential to Reduce Risk of Cardiovascular Diseases

by Claudia Milena Ardila Meléndez

Proceedings 2025, 119(1), 7; https://doi.org/10.3390/proceedings2025119007 - 27 Jun 2025

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Abstract

Cardiovascular diseases (CVDs) remain a leading cause of death globally and in Colombia. Oxidative stress plays a key role in CVD pathogenesis by promoting endothelial damage. Antioxidant compounds from fruits can mitigate oxidative stress through free radical scavenging and metal ion chelation. This [...] Read more.

Cardiovascular diseases (CVDs) remain a leading cause of death globally and in Colombia. Oxidative stress plays a key role in CVD pathogenesis by promoting endothelial damage. Antioxidant compounds from fruits can mitigate oxidative stress through free radical scavenging and metal ion chelation. This review highlights tropical fruits rich in phenolic compounds and evaluates their antioxidant capacity using FRAP and DPPH methods. Evidence supports the inverse relationship between antioxidant intake and CVD incidence. Given Colombia’s fruit diversity, assessing dietary antioxidant capacity may inform strategies to improve diet quality and reduce CVD risk in the population. Full article

(This article belongs to the Proceedings of The 2nd International Electronic Conference on Antioxidants)

6 pages, 461 KB

Open AccessProceeding Paper

Antioxidant and Antitumor Activity Against Colorectal Cancer Cells of Lycium chinense Mill. Cultivated in Ukraine

by Svetlana Lyashenko, Natalia Boiko, Victoriia Soloshenko and José Luis Guil-Guerrero

Proceedings 2025, 119(1), 8; https://doi.org/10.3390/proceedings2025119008 - 7 Jul 2025

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Abstract

The polyphenolic extract from Lycium chinense fruits cultivated in Ukraine was surveyed for antioxidant and anticancer activity against HT-29 colorectal cancer cells. The total phenolic compounds and total flavonoid content were analyzed through the Folin–Ciocalteu and aluminum chloride procedure, respectively, while the antioxidant [...] Read more.

The polyphenolic extract from Lycium chinense fruits cultivated in Ukraine was surveyed for antioxidant and anticancer activity against HT-29 colorectal cancer cells. The total phenolic compounds and total flavonoid content were analyzed through the Folin–Ciocalteu and aluminum chloride procedure, respectively, while the antioxidant activity in vitro was assessed using DPPH radical scavenging and β-carotene bleaching assays. Anticancer effects were established using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The TPC was 11.3 mg GAE/g dry weight, while the TFC was 7.3 mg rutin/g dry weight. The extract demonstrated an antioxidant capacity (DPPH IC₅₀ = 125.9 ± 12.6 µg/mL), lipid peroxidation inhibition (23.5 ± 2.1% in the β-carotene assay) and the inhibition of HT-29 colorectal cancer cells (GI₅₀ at 72 h = 250 µg/mL). These findings provide the first evidence of the anticancer potential of Lycium chinense against human colorectal cancer cells, supporting its application as a source of bioactive compounds for the development of functional foods with antitumor properties. Full article

(This article belongs to the Proceedings of The 2nd International Electronic Conference on Antioxidants)

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7 pages, 395 KB

Open AccessProceeding Paper

Health Effects of Coffee Products on Oxidative Stress-Related Metabolic Disorders: An Updated Perspective

by Santa Anabel Hernández-Abreu and Francisco Javier Álvarez-Martínez

Proceedings 2025, 119(1), 9; https://doi.org/10.3390/proceedings2025119009 - 18 Jul 2025

Viewed by 1183

Abstract

Coffee, rich in polyphenols, has been studied for its impact on obesity and oxidative stress. Its bioactive compounds combat oxidative stress, which is linked to chronic diseases. This study provides a comprehensive, artificial intelligence-enhanced review of the scientific literature, highlighting the impact of [...] Read more.

Coffee, rich in polyphenols, has been studied for its impact on obesity and oxidative stress. Its bioactive compounds combat oxidative stress, which is linked to chronic diseases. This study provides a comprehensive, artificial intelligence-enhanced review of the scientific literature, highlighting the impact of coffee and its derivatives on these disorders. Several studies show that coffee husks and green coffee supplements reduce body weight and inflammation by increasing antioxidant defenses. In conclusion, coffee and its derived products, including valorized by-products, represent a promising avenue for dietary strategies aimed at preventing and managing oxidative stress-associated metabolic disorders and promoting overall metabolic health. Full article

(This article belongs to the Proceedings of The 2nd International Electronic Conference on Antioxidants)

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8 pages, 467 KB

Open AccessProceeding Paper

Role of a Natural Antioxidant in the Secondary Shelf Life of Ready-to-Use Meat Pâté

by Beatrice Sordini, Sonia Esposto, Arianna Bonucci, Ilenia Dottori, Luigi Daidone, Stefania Urbani, Gianluca Veneziani, Roberto Selvaggini, Maurizio Servili, Davide Nucciarelli and Agnese Taticchi

Proceedings 2025, 119(1), 10; https://doi.org/10.3390/proceedings2025119010 - 11 Aug 2025

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Abstract

By-products from the agro-food industry can be natural ingredients for reformulating traditional foods, enhancing quality, extending secondary shelf life (SSL), and reducing food waste. This study evaluates the impact of an olive vegetation water phenolic extract (OVWPE) at two concentrations on ready-to-use meat [...] Read more.

By-products from the agro-food industry can be natural ingredients for reformulating traditional foods, enhancing quality, extending secondary shelf life (SSL), and reducing food waste. This study evaluates the impact of an olive vegetation water phenolic extract (OVWPE) at two concentrations on ready-to-use meat pâté (MP1 and MP2) under simulated retail storage. We evaluated the phenolic composition, volatile compounds, antioxidant activity, and sensory quality of the OVWPE-added pâté and compared it to the control sample (CTRL; without antioxidant). Results indicated that OVWPE minimized oxidation products, enhancing lipid stability, and also reduced the formation of C₆–C₉ aldehydes linked to rancid off-flavor. Without sensory defects, the OVWPE pâtés showed higher antioxidant activity and α-tocopherol content than the CTRL. OVWPE appears to be a promising antioxidant of natural origin for food formulations, supporting SSL extension and food waste reduction. Full article

(This article belongs to the Proceedings of The 2nd International Electronic Conference on Antioxidants)

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0 pages, 578 KB

Open AccessConference Report

Abstracts of the 2nd International Electronic Conference on Antioxidants (IECAN 2025), 7–9 April 2025

by Alessandra Napolitano

Proceedings 2025, 119(1), 11; https://doi.org/10.3390/proceedings2025119011 - 16 Sep 2025

Abstract

The 2nd International Electronic Conference on Antioxidants (IECAN 2025) was organized by MDPI and MDPI Antioxidants. The conference took place online from 7–9 April 2025. This conference report is an abstract collection from six different sessions of IECAN 2025. Full article

(This article belongs to the Proceedings of The 2nd International Electronic Conference on Antioxidants)

8 pages, 650 KB

Open AccessProceeding Paper

Plant-Derived Mucilage: A Natural Antioxidant with Multi-Functional Applications in Food, Cosmetics, and Health

by Mohan Kalaskar and Rajeshwari Patil

Proceedings 2025, 119(1), 12; https://doi.org/10.3390/proceedings2025119012 - 23 Sep 2025

Abstract

Mucilage, naturally occurring polysaccharides in various plant parts, possesses unique structural and multi-functional properties. These biopolymers consist primarily of com-plex polysaccharides associated with flavonoids, phenolics, and oxidized sugars. A systematic review of databases like PubMed, Scopus, and Web of Science evaluated 22 re-search [...] Read more.

Mucilage, naturally occurring polysaccharides in various plant parts, possesses unique structural and multi-functional properties. These biopolymers consist primarily of com-plex polysaccharides associated with flavonoids, phenolics, and oxidized sugars. A systematic review of databases like PubMed, Scopus, and Web of Science evaluated 22 re-search papers on mucilage with antioxidant potential. The key finding highlights that Cydonia ob-longa, Abelmoschus esculentus, Zizyphus mauritiana, Coccinia indica, Hibiscus rosa-sinensis, Malva parviflora, Corchorus olitorius, and Dioscorea opposita contain antioxidants. Various analytical techniques include DPPH, ABTS, FRAP, hydroxyl radical, and superoxide radical assays for the evaluation of the antioxidant properties of mucilage. The findings aim to foster innovation in health benefits and applications in food and cosmetic products, leveraging the multi-functional potential of these biopolymers to enhance efficacy and safety. Full article

(This article belongs to the Proceedings of The 2nd International Electronic Conference on Antioxidants)

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