- Protocol
Expression and Site-Specific Biotinylation of Human Cytosolic 5′-Nucleotidase 1A in Escherichia coli
- Nataliya Slater,
- Anuradha Sooda,
- Frank L. Mastaglia,
- Sue Fletcher,
- Mark Watson,
- Merrilee Needham and
- Jerome D. Coudert
Autoantibodies targeting cytosolic 5′-nucleotidase 1A (cN1A) are found in several autoimmune diseases, including inclusion body myositis (IBM), Sjögren’s syndrome, and systemic lupus erythematosus. While they have diagnostic relevanc...

