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Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis

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Laboratory of Biochemistry and Neurosciences, Department of Biology, University Hassan I, BP 577, 26000 Settat, Morocco
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Laboratory of Applied Chemistry and Environment, Department of Chemistry, University Hassan I, BP 577, 26000 Settat, Morocco
*
Author to whom correspondence should be addressed.
Methods Protoc. 2019, 2(2), 40; https://doi.org/10.3390/mps2020040
Received: 16 March 2019 / Revised: 9 May 2019 / Accepted: 13 May 2019 / Published: 15 May 2019
Tetrahymena pyriformis (protozoa) is intensely investigated as a model organism, offering numerous advantages in comprehensive and multidisciplinary studies using morphologic or molecular methods. Since DNA extraction is a vital step of any molecular experiment, here a new mixed surfactant (Sodium dodecyl sulfate (SDS) 20%/Triton X-100) was adopted for effective DNA extraction from Tetrahymena pyriformis under an easy, fast protocol. The efficiency of this technique was then compared with three widely-used alternative techniques, namely the Chelex 100 matrix, Ammonium pyrrolidine dithiocarbamate (APD) complex and SDS–chloroform methods. DNA extraction was analyzed by pulsed-field gel electrophoresis, spectral measurement, fluorometry (Qubit), restriction enzyme digestion, and polymerase chain reaction. Data analysis revealed that the quantity and quality of the recovered DNA varied depending on the applied DNA extraction method. The new method (SDS 20%/Triton X-100) was the most efficient for extracting DNA from Tetrahymena pyriformis with high integrity and purity, affordable cost, less time, and suitability for molecular applications. View Full-Text
Keywords: Tetrahymena pyriformis; DNA extraction; SDS 20%/Triton X-100; Chelex® 100 matrix; APD complex; SDS-chloroform Tetrahymena pyriformis; DNA extraction; SDS 20%/Triton X-100; Chelex® 100 matrix; APD complex; SDS-chloroform
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MDPI and ACS Style

El Maaiden, E.; El Kharrassi, Y.; Essamadi, A.K.; Moustaid, K.; Nasser, B. Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis. Methods Protoc. 2019, 2, 40.

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