Next Article in Journal
Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis
Next Article in Special Issue
Towards On-Demand E. coli-Based Cell-Free Protein Synthesis of Tissue Plasminogen Activator
Previous Article in Journal
Study Protocol for a Pilot, Open-Label, Prospective, and Observational Study to Evaluate the Pharmacokinetics of Drugs Administered to Patients during Extracorporeal Circulation; Potential of In Vivo Cytochrome P450 Phenotyping to Optimise Pharmacotherapy
Previous Article in Special Issue
Cell-Free Metabolic Engineering: Recent Developments and Future Prospects
Open AccessReview

Cell-Free Synthetic Biology Platform for Engineering Synthetic Biological Circuits and Systems

1
Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, 77 Cheongam-ro, Pohang, Gyeongbuk 37673, Korea
2
Department of Mechanical Engineering, University of California at Riverside, 900 University Ave, Riverside, CA 92521, USA
3
Department of Mechanical and Aerospace Engineering, University of California at Los Angeles, 420 Westwood Plaza, Los Angeles, CA 90095, USA
*
Authors to whom correspondence should be addressed.
Both authors contributed equally to this work.
Methods Protoc. 2019, 2(2), 39; https://doi.org/10.3390/mps2020039
Received: 4 March 2019 / Revised: 12 April 2019 / Accepted: 8 May 2019 / Published: 14 May 2019
(This article belongs to the Special Issue Cell-Free Synthetic Biology)
Synthetic biology integrates diverse engineering disciplines to create novel biological systems for biomedical and technological applications. The substantial growth of the synthetic biology field in the past decade is poised to transform biotechnology and medicine. To streamline design processes and facilitate debugging of complex synthetic circuits, cell-free synthetic biology approaches has reached broad research communities both in academia and industry. By recapitulating gene expression systems in vitro, cell-free expression systems offer flexibility to explore beyond the confines of living cells and allow networking of synthetic and natural systems. Here, we review the capabilities of the current cell-free platforms, focusing on nucleic acid-based molecular programs and circuit construction. We survey the recent developments including cell-free transcription–translation platforms, DNA nanostructures and circuits, and novel classes of riboregulators. The links to mathematical models and the prospects of cell-free synthetic biology platforms will also be discussed. View Full-Text
Keywords: synthetic biology; cell-free transcription-translation; rapid prototyping; artificial cell; riboregulator; DNA origami; mathematical model synthetic biology; cell-free transcription-translation; rapid prototyping; artificial cell; riboregulator; DNA origami; mathematical model
Show Figures

Figure 1

MDPI and ACS Style

Jeong, D.; Klocke, M.; Agarwal, S.; Kim, J.; Choi, S.; Franco, E.; Kim, J. Cell-Free Synthetic Biology Platform for Engineering Synthetic Biological Circuits and Systems. Methods Protoc. 2019, 2, 39.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop