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Structural Characterization of the S-glycosylated Bacteriocin ASM1 from Lactobacillus plantarum

School of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand
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Author to whom correspondence should be addressed.
Current address: Department of Chemistry, University of Wyoming, Laramie, WY 82071, USA.
Magnetochemistry 2020, 6(1), 16; https://doi.org/10.3390/magnetochemistry6010016
Received: 12 October 2019 / Revised: 28 February 2020 / Accepted: 10 March 2020 / Published: 22 March 2020
(This article belongs to the Special Issue Nuclear Magnetic Resonance Spectroscopy in Biomedical Application)
In order to protect their environmental niche, most bacteria secret antimicrobial substances designed to target specific bacterial strains that are often closely related to the producer strain. Bacteriocins, small, ribosomally synthesised antimicrobial peptides, comprise a class of such substances and can either inhibit (bacteriostatic) or kill (bactericidal) target cells. Glycocins are a class of bacteriocin that are post-translationally modified by one or more carbohydrate moieties that are either β-O-linked to either a serine or threonine and/or β-S-linked to a cysteine. The solution nuclear magnetic resonance structure (NMR) of the glycocin ASM1 (produced by Lactobacillus plantarum A-1), an orthologue of GccF, has been determined. In both structures, the disulfide bonds are essential for activity and restrict the mobility of the N-acetyl-glucosamine (GlcNAc) attached to Ser-18 (O-linked), compared to the much more flexible GlcNAc moiety on Cys-43 (S-linked). Interestingly, despite 88% sequence identity, the helical structure of ASM1 is less pronounced which appears to be consistent with the far ultra-violet circular dichroism (UV CD) spectra. View Full-Text
Keywords: NMR; circular dichroism; bacteriocin; post-translational modifications; S-linked glycosylation; O-linked glycosylation NMR; circular dichroism; bacteriocin; post-translational modifications; S-linked glycosylation; O-linked glycosylation
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MDPI and ACS Style

Goroncy, A.K.; Loo, T.S.; Koolaard, A.M.; Patchett, M.L.; Norris, G.E. Structural Characterization of the S-glycosylated Bacteriocin ASM1 from Lactobacillus plantarum. Magnetochemistry 2020, 6, 16.

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