Application of Cool Fermentation Temperatures to Encourage Non-Saccharomyces Yeasts to Yield Lower Ethanol Concentrations in Wines

Application of cool temperatures were studied to encourage Metschnikowia pulcherrima P01A016 and Meyerozyma guilliermondii P40D002 prior inoculation of Saccharomyces cerevisiae D254 to lower ultimate ethanol concentrations achieved. Merlot grape must was distributed into 300 L temperature-controlled tanks and inoculated with non-Saccharomyces yeasts three days before S. cerevisiae. For control fermentations, S. cerevisiae was inoculated with maximum temperatures set to 25 °C (temperature regime I) while those with Mt. pulcherrima or My. guilliermondii were initially set to 15 °C (temperature regime II) or 17.5 °C (temperature regime III) before increasing to 25 °C after adding S. cerevisiae. Once fermentations achieved dryness (≤2 g/L residual sugar), wines were bottled and stored for six months at 7 °C before sensory analysis. Ethanol reduction by Mt. pulcherrima was not observed in wines fermented under II but was by III (0.8% v/v). In contrast, musts inoculated with My. guilliermondii yielded wines with ethanol concentrations lowered by 0.3% (II) or 0.4% v/v (III). Sensory panelists found wines with Mt. pulcherrima to express lower sensory scores for ‘hotness’, ‘bitterness’, and ‘ethanol’ flavor with fewer differences noted for My. guilliermondii. Reducing final ethanol concentrations of Merlot wines were achieved by Mt. pulcherrima or My. guilliermondii using cooler initial fermentation temperatures without adversely affecting final wine quality.