In this study, an innovative green biorefinery system was successfully developed to process the green biomass into multiple biofuels and bioproducts. In particular, fresh giant miscanthus was separated into a solid stream (press cake) and a liquid stream (press juice) using a screw press. The juice was used to cultivate microalga Chlorella vulgaris
, which was further thermochemically converted via thermogravimetry analysis (TGA) and pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) analysis, resulting in an approximately 80% conversion. In addition, the solid cake of miscanthus was pretreated with dilute sulfuric acid and used as the feedstock for bioethanol production. The results showed that the miscanthus juice could be a highly nutritious source for microalgae that are a promising feedstock for biofuels. The highest cell density was observed in the 15% juice medium. Sugars released from the miscanthus cake were efficiently fermented to ethanol using Saccharomyces cerevisiae
through a simultaneous saccharification and fermentation (SSF) process, with 88.4% of the theoretical yield.
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