Gels, Volume 10, Issue 9
2024 September - 65 articles
Cover Story: Hydrogels are essential in tissue engineering due to their 3D structure and ability to support cellular functions, but dextran-based hydrogels lack intrinsic biological activity. This study introduces bioactive hydrogels using sequential thiol–maleimide bio-functionalization and enzyme-catalyzed crosslinking. The hydrogel network forms through the crosslinking of tyramine moieties with horseradish peroxidase (HRP) and hydrogen peroxide (H2O2), enabling tunable gelation and stiffness. Maleimide groups allow the coupling of thiol-containing bioactive molecules like RGD peptides to enhance biological activity. Human mesenchymal stem cells (hMSCs) showed over 88% viability on day 1 and 60–81% by day 14, with adipogenic differentiation observed. Hence, DexTA–Mal hydrogels offer a biocompatible, adaptable platform for tissue engineering. View this paper - Issues are regarded as officially published after their release is announced to the table of contents alert mailing list .
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