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Article

Isolation and Characterization of Feline Wharton’s Jelly-Derived Mesenchymal Stem Cells

by 1,†, 1,†, 1, 1, 1,2, 3,*,‡ and 3,*,‡
1
Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 41061, Korea
2
Department of Veterinary Toxicology, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea
3
Department of Veterinary Surgery, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea
*
Authors to whom correspondence should be addressed.
Seo and Kang contributed equally to this work as co-first authors.
Yun and Kwon contributed equally to this work as co-corresponding authors.
Academic Editor: Mark Brown
Vet. Sci. 2021, 8(2), 24; https://doi.org/10.3390/vetsci8020024
Received: 5 January 2021 / Revised: 2 February 2021 / Accepted: 2 February 2021 / Published: 7 February 2021
Wharton’s jelly is a well-known mesenchymal stem cell source in many species, including humans. However, there have been no reports confirming the presence of mesenchymal stem cells in Wharton’s jelly in cats. The purpose of this study was to isolate mesenchymal stem cells (MSCs) from the Wharton’s jelly of cats and to characterize stem cells. In this study, feline Wharton’s jelly-derived mesenchymal stem cells (fWJ-MSCs) were isolated and successfully cultured. fWJ-MSCs were maintained and the proliferative potential was measured by cumulative population doubling level (CPDL) test, scratch test, and colony forming unit (CFU) test. Stem cell marker, karyotyping and immunophenotyping analysis by flow cytometry showed that fWJ-MSCs possessed characteristic mesenchymal stem cell markers. To confirm the differentiation potential, we performed osteogenic, adipogenic and chondrogenic induction under each differentiation condition. fWJ-MSCs has the ability to differentiate into multiple lineages, including osteogenic, adipogenic and chondrogenic differentiation. This study shows that Wharton’s jelly of cat can be a good source of mesenchymal stem cells. In addition, fWJ-MSCs may be useful for stem cell-based therapeutic applications in feline medicine. View Full-Text
Keywords: feline; Wharton’s jelly; mesenchymal stem cell; characterization feline; Wharton’s jelly; mesenchymal stem cell; characterization
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MDPI and ACS Style

Seo, M.-S.; Kang, K.-K.; Oh, S.-K.; Sung, S.-E.; Kim, K.-S.; Kwon, Y.-S.; Yun, S. Isolation and Characterization of Feline Wharton’s Jelly-Derived Mesenchymal Stem Cells. Vet. Sci. 2021, 8, 24. https://doi.org/10.3390/vetsci8020024

AMA Style

Seo M-S, Kang K-K, Oh S-K, Sung S-E, Kim K-S, Kwon Y-S, Yun S. Isolation and Characterization of Feline Wharton’s Jelly-Derived Mesenchymal Stem Cells. Veterinary Sciences. 2021; 8(2):24. https://doi.org/10.3390/vetsci8020024

Chicago/Turabian Style

Seo, Min-Soo, Kyung-Ku Kang, Se-Kyung Oh, Soo-Eun Sung, Kil-Soo Kim, Young-Sam Kwon, and Sungho Yun. 2021. "Isolation and Characterization of Feline Wharton’s Jelly-Derived Mesenchymal Stem Cells" Veterinary Sciences 8, no. 2: 24. https://doi.org/10.3390/vetsci8020024

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