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Bioengineering, Volume 8, Issue 4

April 2021 - 7 articles

Cover Story: Lipocalin 2 (Lcn2) overexpression can lead to cancer progression by enhancing tumor angiogenesis. In this study, we engineered a PEGylated liposomal system encapsulating Lcn2 small interfering RNA (Lcn2 siRNA). The PEGylated liposomes were decorated with octreotide (OCT) peptide for targeting breast cancer cells. Optimized OCT-targeted Lcn2 siRNA liposomes (OCT-Lcn2-Lipo) had a mean size of 152 nm and 69.5% encapsulation efficiency. OCT-Lcn2-lipo could achieve 55−60% silencing of Lcn2 mRNA in MCF-7 and MDA-MB-231 cells. In addition, OCT-Lcn2-Lipo demonstrated in vitro anti-angiogenic effects by reducing VEGF-A levels in treated cells. OCT-Lcn2-lipo also reduced the endothelial cell (HUVEC) migration levels. This approach may be useful in inhibiting angiogenesis and metastasis in breast cancer. View this paper
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Articles (7)

  • Review
  • Open Access
27 Citations
10,296 Views
29 Pages

The novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused respiratory infection, resulting in more than two million deaths globally and hospitalizing thousands of people by March 2021. A considerable percentage of the SARS-CoV...

  • Review
  • Open Access
45 Citations
8,406 Views
26 Pages

Protein-Based 3D Biofabrication of Biomaterials

  • Mahta Mirzaei,
  • Oseweuba Valentine Okoro,
  • Lei Nie,
  • Denise Freitas Siqueira Petri and
  • Amin Shavandi

Protein/peptide-based hydrogel biomaterial inks with the ability to incorporate various cells and mimic the extracellular matrix’s function are promising candidates for 3D printing and biomaterials engineering. This is because proteins contain multip...

  • Article
  • Open Access
18 Citations
5,934 Views
14 Pages

Lower Limb Kinematics in Individuals with Hip Osteoarthritis during Gait: A Focus on Adaptative Strategies and Interlimb Symmetry

  • Micaela Porta,
  • Massimiliano Pau,
  • Bruno Leban,
  • Michela Deidda,
  • Marco Sorrentino,
  • Federico Arippa and
  • Giuseppe Marongiu

Among the functional limitations associated with hip osteoarthritis (OA), the alteration of gait capabilities represents one of the most invalidating as it may seriously compromise the quality of life of the affected individual. The use of quantitati...

  • Review
  • Open Access
5 Citations
6,253 Views
15 Pages

It is widely accepted that chondral defects in articular cartilage of adult joints are never repaired spontaneously, which is considered to be one of the major causes of age-related degenerative joint disorders, such as osteoarthritis. Since mobiliza...

  • Article
  • Open Access
13 Citations
3,978 Views
15 Pages

Biomechanically Tunable Nano-Silica/P-HEMA Structural Hydrogels for Bone Scaffolding

  • Raffaella Aversa,
  • Relly Victoria Petrescu,
  • Florian Ion T. Petrescu,
  • Valeria Perrotta,
  • Davide Apicella and
  • Antonio Apicella

Innovative tissue engineering biomimetic hydrogels based on hydrophilic polymers have been investigated for their physical and mechanical properties. 5% to 25% by volume loading PHEMA-nanosilica glassy hybrid samples were equilibrated at 37 °C in aqu...

  • Article
  • Open Access
21 Citations
6,643 Views
25 Pages

Lcn2 overexpression in metastatic breast cancer (MBC) can lead to cancer progression by inducing the epithelial-to-mesenchymal transition and enhancing tumor angiogenesis. In this study, we engineered a PEGylated liposomal system encapsulating lipoca...

  • Article
  • Open Access
6 Citations
4,628 Views
13 Pages

Dolastatin derivatives, represented by monomethylauristatin E (MMAE), have been translated in clinic with a form of antibody–drug conjugate; however, their potential in nanoparticle systems has not been well established due to the potential risk of i...

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Bioengineering - ISSN 2306-5354