Next Article in Journal
Evaluation of Garlic Landraces from Foggia Province (Puglia Region; Italy)
Next Article in Special Issue
In Vitro Modulation of Gut Microbiota and Metabolism by Cooked Cowpea and Black Bean
Previous Article in Journal
A Preliminary Report for the Design of MoS (Micro-Olive-Spreadsheet), a User-Friendly Spreadsheet for the Evaluation of the Microbiological Quality of Spanish-Style Bella di Cerignola Olives from Apulia (Southern Italy)
Previous Article in Special Issue
Quantitative Analysis of Antibody Survival across the Infant Digestive Tract Using Mass Spectrometry with Parallel Reaction Monitoring
Open AccessCommunication

Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 Mycotoxicosis

Faculty of Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia
Faculty of Veterinary Medicine, Ss.Cyril & Methodius University in Skopje, 1000 Skopje, North Macedonia
Laboratory for Molecular Food Analyses and Genetically Modified Organism, Food Institute, Faculty of Veterinary Medicine, 1000 Skopje, North Macedonia
Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia
Faculty of Veterinary Medicine, Latvian University of Agriculture, LV 3004 Jelgava, Latvia
Author to whom correspondence should be addressed.
Foods 2020, 9(7), 849;
Received: 5 May 2020 / Revised: 28 May 2020 / Accepted: 26 June 2020 / Published: 29 June 2020
(This article belongs to the Special Issue New Challenges and Opportunities of Food Digestion)
Although patterns of glucose transporter expression and notes about diseases leading to adaptive changes in intestinal fructose transport have been well-characterized, the connection between infection and fructose transportation has been lightly investigated. Up to now only few studies on GLUT-5 expression and function under pathological conditions in bird intestines have been carried out. The aim of our current research was to immunolocalize GLUT-5 in chicken duodenal epithelium in norm and during T-2 mycotoxicosis. Material from chicken (Gallus gallus domesticus) duodenum was collected from twelve seven-day-old female broilers, divided into control group and broilers with T-2 mycotoxicosis. The material was fixed with 10% formalin and thereafter embedded into paraffin; slices 7 μm in thickness were cut, followed by immunohistochemical staining, according to the manufacturers guidelines (IHC kit, Abcam, UK) using polyclonal primary antibody Rabbit anti-GLUT-5. Our study revealed the strong expression of GLUT-5 in the apical parts of the duodenal epithelial cells in the control group chickens and weak staining for GLUT-5 in the intestinal epithelium in the T-2 mycotoxicosis group. Our results confirmed decreased the expression of GLUT-5 in the duodenal epithelium during T-2 mycotoxicosis. View Full-Text
Keywords: GLUT-5; duodenal epithelium; T-2 mycotoxicosis GLUT-5; duodenal epithelium; T-2 mycotoxicosis
Show Figures

Graphical abstract

MDPI and ACS Style

Hussar, P.; Popovska-Percinic, F.; Blagoevska, K.; Järveots, T.; Dūrītis, I. Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 Mycotoxicosis. Foods 2020, 9, 849.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop