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Open AccessCommunication

Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 Mycotoxicosis

1
Faculty of Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia
2
Faculty of Veterinary Medicine, Ss.Cyril & Methodius University in Skopje, 1000 Skopje, North Macedonia
3
Laboratory for Molecular Food Analyses and Genetically Modified Organism, Food Institute, Faculty of Veterinary Medicine, 1000 Skopje, North Macedonia
4
Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia
5
Faculty of Veterinary Medicine, Latvian University of Agriculture, LV 3004 Jelgava, Latvia
*
Author to whom correspondence should be addressed.
Foods 2020, 9(7), 849; https://doi.org/10.3390/foods9070849
Received: 5 May 2020 / Revised: 28 May 2020 / Accepted: 26 June 2020 / Published: 29 June 2020
(This article belongs to the Special Issue New Challenges and Opportunities of Food Digestion)
Although patterns of glucose transporter expression and notes about diseases leading to adaptive changes in intestinal fructose transport have been well-characterized, the connection between infection and fructose transportation has been lightly investigated. Up to now only few studies on GLUT-5 expression and function under pathological conditions in bird intestines have been carried out. The aim of our current research was to immunolocalize GLUT-5 in chicken duodenal epithelium in norm and during T-2 mycotoxicosis. Material from chicken (Gallus gallus domesticus) duodenum was collected from twelve seven-day-old female broilers, divided into control group and broilers with T-2 mycotoxicosis. The material was fixed with 10% formalin and thereafter embedded into paraffin; slices 7 μm in thickness were cut, followed by immunohistochemical staining, according to the manufacturers guidelines (IHC kit, Abcam, UK) using polyclonal primary antibody Rabbit anti-GLUT-5. Our study revealed the strong expression of GLUT-5 in the apical parts of the duodenal epithelial cells in the control group chickens and weak staining for GLUT-5 in the intestinal epithelium in the T-2 mycotoxicosis group. Our results confirmed decreased the expression of GLUT-5 in the duodenal epithelium during T-2 mycotoxicosis. View Full-Text
Keywords: GLUT-5; duodenal epithelium; T-2 mycotoxicosis GLUT-5; duodenal epithelium; T-2 mycotoxicosis
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MDPI and ACS Style

Hussar, P.; Popovska-Percinic, F.; Blagoevska, K.; Järveots, T.; Dūrītis, I. Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 Mycotoxicosis. Foods 2020, 9, 849.

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