A New Edible Film to Produce In Vitro Meat
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microstructured Mold Fabrication
2.2. Edible Film Preparation
2.3. Cell Culture
2.4. Cell Proliferation
2.5. Microscopy
2.6. Spreading Assay
2.7. Myogenic Differentation
2.8. Gene Expression
2.9. Statistics
3. Results and Discussion
3.1. Micromolding
3.2. Proliferation of Muscle Fiber-like Biomass
3.3. Expression of Genes of Myogenic Differentiation
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Gene | Name | Accesion GenBank | Primer Sequences | Amplicon Length |
---|---|---|---|---|
Des | Desmin | NM_010043.2 | Fw: 5’-GATGCAGCCACTCTAGCTCGTATT-3’ Rv: 5’-TTCTTAGCCGCGATGGTCTCATAC-3’ | 218 bp |
Myog | Myogenin | NM_031189.2 | Fw: 5’-AGAAGCGCAGGCTCAAGAAAGT-3’ Rv: 5’-AGTTGCATTCACTGGGCACCAT-3’ | 222 bp |
Myh2 | Myosin heavy chain | NM_001039545.2 | Fw: 5’-CCTCTTATTTCCCAGCTGCACCTT-3’ Rv: 5’-GTCACTTTCCCTGCATCTTTGCTC-3’ | 242 bp |
PBGD | Porphobilinogen deaminase | NM_001110251.1 | Fw: 5’-TGGCGATGCTGAAAGCCTTGTA-3’ Rv: 5’-GTTTTCCCGTTTGCAGATGGCT-3’ | 239 bp |
Eef2 | Eukaryotic translation elongation factor 2 | NM_007907.2 | Fw: 5’-ATCGTGGAGAACGTCAACGTCA-3’ Rv: 5’-TGCCATTGGCCGGATCAAAGTA-3’ | 274 bp |
Gene | Surface | Days of Differentiation | ΔCT (Max-Min) | Coefficient of Variation | ||
---|---|---|---|---|---|---|
0 | 7 | 14 | ||||
PBGD | Commercial Flask | 25.5 (±0.08) | 25.3 (±0.06) | 25.2 (±0.01) | 0.3 | 0.007 |
Flat Film | 26.8 (±0.29) | 27.0 (±0.11) | 27.4 (±0.00) | 0.6 | 0.011 | |
Microstructured film | 27.2 (±0.11) | 26.6 (±0.15) | 27.2 (±0.30) | 0.6 | 0.013 | |
Eef2 | Commercial Flask | 20.7 (±0.06) | 19.9 (±0.12) | 20.2 (±0.37) | 0.8 | 0.020 |
Flat Film | 21.8 (±0.30) | 21.8 (±0.01) | 22.4 (±1.78) | 0.6 | 0.016 | |
Microstructured film | 22.6 (±0.02) | 20.9 (±0.13) | 21.2 (±0.01) | 1.7 | 0.042 |
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Orellana, N.; Sánchez, E.; Benavente, D.; Prieto, P.; Enrione, J.; Acevedo, C.A. A New Edible Film to Produce In Vitro Meat. Foods 2020, 9, 185. https://doi.org/10.3390/foods9020185
Orellana N, Sánchez E, Benavente D, Prieto P, Enrione J, Acevedo CA. A New Edible Film to Produce In Vitro Meat. Foods. 2020; 9(2):185. https://doi.org/10.3390/foods9020185
Chicago/Turabian StyleOrellana, Nicole, Elizabeth Sánchez, Diego Benavente, Pablo Prieto, Javier Enrione, and Cristian A. Acevedo. 2020. "A New Edible Film to Produce In Vitro Meat" Foods 9, no. 2: 185. https://doi.org/10.3390/foods9020185