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Article

Antibiofilm Effect of DNase against Single and Mixed Species Biofilm

1
Ashok & Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New Vallabh Vidya Nagar, Anand 388121, Gujarat, India
2
Department of Food Processing Technology, AD Patel Institute of Technology, New Vallabh Vidya Nagar, Anand 388121, Gujarat, India
*
Author to whom correspondence should be addressed.
Foods 2018, 7(3), 42; https://doi.org/10.3390/foods7030042
Received: 30 November 2017 / Revised: 14 March 2018 / Accepted: 15 March 2018 / Published: 19 March 2018
Biofilms are aggregates of microorganisms that coexist in socially coordinated micro-niche in a self-produced polymeric matrix on pre-conditioned surfaces. The biofilm matrix reduces the efficacy of antibiofilm strategies. DNase degrades the extracellular DNA (e-DNA) present in the matrix, rendering the matrix weak and susceptible to antimicrobials. In the current study, the effect of DNase I was evaluated during biofilm formation (pre-treatment), on preformed biofilms (post-treatment) and both (dual treatment). The DNase I pre-treatment was optimized for P. aeruginosa PAO1 (model biofilm organism) at 10 µg/mL and post-treatment at 10 µg/mL with 15 min of contact duration. Inclusion of Mg2+ alongside DNase I post-treatment resulted in 90% reduction in biofilm within only 5 min of contact time (irrespective of age of biofilm). On extension of these findings, DNase I was found to be less effective against mixed species biofilm than individual biofilms. DNase I can be used as potent antibiofilm agent and with further optimization can be effectively used for biofilm prevention and reduction in situ. View Full-Text
Keywords: biofilms; DNase I; pre-treatment; post-treatment; mixed species biofilm; disintegration of matrix; antibiofilm methods biofilms; DNase I; pre-treatment; post-treatment; mixed species biofilm; disintegration of matrix; antibiofilm methods
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MDPI and ACS Style

Sharma, K.; Pagedar Singh, A. Antibiofilm Effect of DNase against Single and Mixed Species Biofilm. Foods 2018, 7, 42. https://doi.org/10.3390/foods7030042

AMA Style

Sharma K, Pagedar Singh A. Antibiofilm Effect of DNase against Single and Mixed Species Biofilm. Foods. 2018; 7(3):42. https://doi.org/10.3390/foods7030042

Chicago/Turabian Style

Sharma, Komal, and Ankita Pagedar Singh. 2018. "Antibiofilm Effect of DNase against Single and Mixed Species Biofilm" Foods 7, no. 3: 42. https://doi.org/10.3390/foods7030042

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