Next Article in Journal
Effect of Oregano Essential Oil and Aqueous Oregano Infusion Application on Microbiological Properties of Samarella (Tsamarella), a Traditional Meat Product of Cyprus
Next Article in Special Issue
Current Knowledge on Listeria monocytogenes Biofilms in Food-Related Environments: Incidence, Resistance to Biocides, Ecology and Biocontrol
Previous Article in Journal
Chemical Composition and Antioxidant Activity of Propolis Prepared in Different Forms and in Different Solvents Useful for Finished Products
Previous Article in Special Issue
Effect of Food Residues in Biofilm Formation on Stainless Steel and Polystyrene Surfaces by Salmonella enterica Strains Isolated from Poultry Houses
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Foods 2018, 7(3), 42; https://doi.org/10.3390/foods7030042

Antibiofilm Effect of DNase against Single and Mixed Species Biofilm

1
Ashok & Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New Vallabh Vidya Nagar, Anand 388121, Gujarat, India
2
Department of Food Processing Technology, AD Patel Institute of Technology, New Vallabh Vidya Nagar, Anand 388121, Gujarat, India
*
Author to whom correspondence should be addressed.
Received: 30 November 2017 / Revised: 14 March 2018 / Accepted: 15 March 2018 / Published: 19 March 2018
Full-Text   |   PDF [1028 KB, uploaded 3 May 2018]   |  

Abstract

Biofilms are aggregates of microorganisms that coexist in socially coordinated micro-niche in a self-produced polymeric matrix on pre-conditioned surfaces. The biofilm matrix reduces the efficacy of antibiofilm strategies. DNase degrades the extracellular DNA (e-DNA) present in the matrix, rendering the matrix weak and susceptible to antimicrobials. In the current study, the effect of DNase I was evaluated during biofilm formation (pre-treatment), on preformed biofilms (post-treatment) and both (dual treatment). The DNase I pre-treatment was optimized for P. aeruginosa PAO1 (model biofilm organism) at 10 µg/mL and post-treatment at 10 µg/mL with 15 min of contact duration. Inclusion of Mg2+ alongside DNase I post-treatment resulted in 90% reduction in biofilm within only 5 min of contact time (irrespective of age of biofilm). On extension of these findings, DNase I was found to be less effective against mixed species biofilm than individual biofilms. DNase I can be used as potent antibiofilm agent and with further optimization can be effectively used for biofilm prevention and reduction in situ. View Full-Text
Keywords: biofilms; DNase I; pre-treatment; post-treatment; mixed species biofilm; disintegration of matrix; antibiofilm methods biofilms; DNase I; pre-treatment; post-treatment; mixed species biofilm; disintegration of matrix; antibiofilm methods
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Sharma, K.; Pagedar Singh, A. Antibiofilm Effect of DNase against Single and Mixed Species Biofilm. Foods 2018, 7, 42.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Foods EISSN 2304-8158 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top