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21 January 2026

Deep Eutectic Solvents Mediated Extraction of a Pectin Polysaccharide from Processed Sweet Potato By-Products: Optimization and Characterization Studies

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1
School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, China
2
Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Key Laboratory of Biology and Genetic Breeding of Sweet Potato, Ministry of Agriculture and Rural Affairs/Sweet Potato Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Xuzhou 221131, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
This article belongs to the Section Food Physics and (Bio)Chemistry

Abstract

In this study, a pectin polysaccharide named DESP was extracted using a deep eutectic solvent (DES) from sweet potato residue (SPR) and the extract was optimized through response surface methodology (RSM). The DESP, based on choline chloride–urea (ChCl-Ur), was characterized for yield, molecular weight (Mw), and monosaccharide composition. Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), 1H-nuclearmagnetic resonance (1H-NMR), and scanning electron microscopy (SEM) were used to analyze the structure. Optimal extraction conditions for DESP were ChCl-Ur in a molar ratio of 1:2, water content of 75 wt.%, extraction time of 125.7 min, extraction temperature of 83.2 °C, and a liquid-to-solid ratio of 37.0 mL·g−1. The optimized extraction yield was 5.6% ± 0.09%, which was 2.4 times higher than that of hot-water-extracted sweet potato pectin (HWSP, 2.32%). The monosaccharide analysis revealed that galacturonic acid (GalA) was the most abundant saccharide, followed by glucose (Glc), galactose (Gal), arabinose (Ara), and rhamnose (Rha). The Mw of DESP was 20.90 kDa, which was lower than that of HWSP and HASP. In addition, DESP exhibited certain anti-inflammatory activity.

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