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Technical Note

High-Throughput Mass Spectrometry Applied to Structural Genomics

Structural Genomics Consortium, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Chromatography 2014, 1(4), 159-175; https://doi.org/10.3390/chromatography1040159
Received: 1 July 2014 / Revised: 3 September 2014 / Accepted: 11 September 2014 / Published: 9 October 2014
Mass spectrometry (MS) remains under-utilized for the analysis of expressed proteins because it is inaccessible to the non-specialist, and sample-turnaround from service labs is slow. Here, we describe 3.5 min Liquid-Chromatography (LC)-MS and 16 min LC-MSMS methods which are tailored to validation and characterization of recombinant proteins in a high throughput structural biology pipeline. We illustrate the type and scope of MS data typically obtained from a 96-well expression and purification test for both soluble and integral membrane proteins (IMPs), and describe their utility in the selection of constructs for scale-up structural work, leading to cost and efficiency savings. We propose that value of MS data lies in how quickly it becomes available and that this can fundamentally change the way in which it is used. View Full-Text
Keywords: high-throughput; mass spectrometry; protein expression; structural genomics; integral membrane protein (IMP); post-translational modification (PTM); intact mass high-throughput; mass spectrometry; protein expression; structural genomics; integral membrane protein (IMP); post-translational modification (PTM); intact mass
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MDPI and ACS Style

Chalk, R.; Berridge, G.; Shrestha, L.; Strain-Damerell, C.; Mahajan, P.; Yue, W.W.; Gileadi, O.; Burgess-Brown, N.A. High-Throughput Mass Spectrometry Applied to Structural Genomics. Chromatography 2014, 1, 159-175. https://doi.org/10.3390/chromatography1040159

AMA Style

Chalk R, Berridge G, Shrestha L, Strain-Damerell C, Mahajan P, Yue WW, Gileadi O, Burgess-Brown NA. High-Throughput Mass Spectrometry Applied to Structural Genomics. Chromatography. 2014; 1(4):159-175. https://doi.org/10.3390/chromatography1040159

Chicago/Turabian Style

Chalk, Rod, Georgina Berridge, Leela Shrestha, Claire Strain-Damerell, Pravin Mahajan, Wyatt W. Yue, Opher Gileadi, and Nicola A. Burgess-Brown 2014. "High-Throughput Mass Spectrometry Applied to Structural Genomics" Chromatography 1, no. 4: 159-175. https://doi.org/10.3390/chromatography1040159

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