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Open AccessArticle

An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite Green

Graduate School of Human Life Science, Osaka City University, Osaka 558-8585, Japan
Chromatography 2014, 1(2), 75-81; https://doi.org/10.3390/chromatography1020075
Received: 14 April 2014 / Revised: 15 May 2014 / Accepted: 16 May 2014 / Published: 27 May 2014
This paper describes a reserved-phase high-performance liquid chromatographic (HPLC) method for detecting malachite green (MG) and leuco-malachite green (LMG) using an isocratic toxic organic solvent/reagent-free mobile phase. Chromatographic separations were performed an Inertsil® WP300 C4 with 0.02 mol/L octane sulfonic acid–ethanol mobile phase and a photodiode-array detector. The total run time was <5 min. The system suitability was well within the international acceptance criteria. A harmless method for simultaneously detecting MG and LMG was developed and may be further applied to the quantification in foods. View Full-Text
Keywords: internal harmonized analytical method; malachite green; leuco-malachite green; high-performance liquid chromatography; photo-diode array internal harmonized analytical method; malachite green; leuco-malachite green; high-performance liquid chromatography; photo-diode array
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Furusawa, N. An Isocratic Toxic Chemical-Free Mobile Phase HPLC-PDA Analysis of Malachite Green and Leuco-Malachite Green. Chromatography 2014, 1, 75-81.

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