Glomerular Mesangial Cell pH Homeostasis Mediates Mineralocorticoid Receptor-Induced Cell Proliferation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Determination of Cytosolic pH
2.3. Determination of Cytosolic Free Calcium
2.4. Determination of Cell Number
2.5. Thymidine Incorporation
2.6. Determination of IP3 Formation
2.7. Determination of Collagen Secretion by Collagenase-Sensitive Proline Incorporation
2.8. Western Blot
2.9. Materials
2.10. Statistics
3. Results
3.1. Aldosterone-Induced Changes in Cytosolic pH
3.2. Pharmacology of Aldosterone-Induced Changes in Cytosolic pH
3.3. Aldosterone-Induced Cell Proliferation and Collagen Secretion
3.4. No Contribution of Cytosolic Ca2+ to Aldosterone-Induced Alkalinisation or Cell Proliferation
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gekle, M.; Mildenberger, S. Glomerular Mesangial Cell pH Homeostasis Mediates Mineralocorticoid Receptor-Induced Cell Proliferation. Biomedicines 2021, 9, 1117. https://doi.org/10.3390/biomedicines9091117
Gekle M, Mildenberger S. Glomerular Mesangial Cell pH Homeostasis Mediates Mineralocorticoid Receptor-Induced Cell Proliferation. Biomedicines. 2021; 9(9):1117. https://doi.org/10.3390/biomedicines9091117
Chicago/Turabian StyleGekle, Michael, and Sigrid Mildenberger. 2021. "Glomerular Mesangial Cell pH Homeostasis Mediates Mineralocorticoid Receptor-Induced Cell Proliferation" Biomedicines 9, no. 9: 1117. https://doi.org/10.3390/biomedicines9091117