Next Article in Journal
Deposition Time and Annealing Effects of ZnO Seed Layer on Enhancing Vertical Alignment of Piezoelectric ZnO Nanowires
Previous Article in Journal
Fluorescence-Based Detection of Benzene, Toluene, Ethylbenzene, Xylene, and Cumene (BTEXC) Compounds in Fuel-Contaminated Snow Environments
Previous Article in Special Issue
Inkjet-Printed Wireless Chemiresistive Sensors—A Review
Article Menu

Export Article

Open AccessArticle
Chemosensors 2019, 7(1), 6; https://doi.org/10.3390/chemosensors7010006

Impedance Study of Dopamine Effects after Application on 2D and 3D Neuroblastoma Cell Cultures Developed on a 3D-Printed Well

1
Laboratory of Cell Technology, Department of Biotechnology, Agricultural University of Athens, 118 55Athens, Greece
2
microSENSES Laboratory, Department of Electrical and Electronic Engineering, University of West Attica, 122 44 Athens, Greece
3
Institute of Nanoscience and Nanotechnology (INN), National Centre for Scientific Research “Demokritos”, 153 41 Athens, Greece
*
Author to whom correspondence should be addressed.
Received: 30 November 2018 / Revised: 25 January 2019 / Accepted: 30 January 2019 / Published: 5 February 2019
(This article belongs to the Special Issue Printed Electroanalytical Tools for De-Centralized Applications)
Full-Text   |   PDF [4703 KB, uploaded 16 February 2019]   |  

Abstract

In this work, the assessment of the interactions of a bioactive substance applied to immobilized cells in either a two-dimensional (2D) or three-dimensional (3D) arrangement mimicking in vivo tissue conditions is presented. In particular, dopamine (DA) was selected as a stimulant for the implementation of an impedance analysis with a specific type of neural cells (murine neuroblastoma). The aim of this study was the extraction of calibration curves at various frequencies with different known dopamine concentrations for the description of the behavior of dopamine applied to 2D and 3D cell cultures. The results present the evaluation of the mean impedance value for each immobilization technique in each frequency. The differential responses showed the importance of the impedance when frequency is applied in both 2D and 3D immobilization cases. More specifically, in 2D immobilization matrix impedance shows higher values in comparison with the 3D cell culture. Additionally, in the 3D case, the impedance decreases with increasing concentration, while in the 2D case, an opposite behavior was observed. View Full-Text
Keywords: impedance study; dopamine; immobilization; N2a cells impedance study; dopamine; immobilization; N2a cells
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Paivana, G.; Apostolou, T.; Mavrikou, S.; Barmpakos, D.; Kaltsas, G.; Kintzios, S. Impedance Study of Dopamine Effects after Application on 2D and 3D Neuroblastoma Cell Cultures Developed on a 3D-Printed Well. Chemosensors 2019, 7, 6.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Chemosensors EISSN 2227-9040 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top