IR-783 Labeling of a Peptide Receptor for ‘Turn-On’ Fluorescence Based Sensing
AbstractIn this study, we examine a means for developing near-IR fluorescent sensors through streamlined, site-specific coupling with peptide-based receptors. As the penultimate step of solid-phase synthesis of a peptide-based receptor, we show a simple means of labeling the N’ terminus with the near IR fluorophore IR-783 to afford a viable fluorescent sensor after cleavage from the resin. The proof-of-concept probe utilized a biotin mimetic peptide sequence as the receptive moiety. Here we revealed a “turn-on” fluorescence enhancement upon binding of the biotin mimetic probe to its intended streptavidin target. Not all peptide-receptive moieties tested were able to generate such an enhancement upon target binding, and as such, the rationale for the observed fluorescence response properties is discussed. View Full-Text
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Kwak, E.-A.; Kydd, L.; Lim, B.; Jaworski, J. IR-783 Labeling of a Peptide Receptor for ‘Turn-On’ Fluorescence Based Sensing. Chemosensors 2018, 6, 47.
Kwak E-A, Kydd L, Lim B, Jaworski J. IR-783 Labeling of a Peptide Receptor for ‘Turn-On’ Fluorescence Based Sensing. Chemosensors. 2018; 6(4):47.Chicago/Turabian Style
Kwak, Eun-A; Kydd, LeNaiya; Lim, Butaek; Jaworski, Justyn. 2018. "IR-783 Labeling of a Peptide Receptor for ‘Turn-On’ Fluorescence Based Sensing." Chemosensors 6, no. 4: 47.
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