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Chemosensors 2018, 6(4), 47;

IR-783 Labeling of a Peptide Receptor for ‘Turn-On’ Fluorescence Based Sensing

Department of Pharmacology, College of Medicine, University of Arizona, 1501 N. Campbell Ave., Tucson, AZ 85724, USA
Department of Bioengineering, University of Texas, Arlington, 500 UTA Blvd., Arlington, TX 76010, USA
Author to whom correspondence should be addressed.
Received: 22 August 2018 / Revised: 27 September 2018 / Accepted: 12 October 2018 / Published: 16 October 2018
(This article belongs to the Special Issue Aptamer Technologies)
PDF [1131 KB, uploaded 16 October 2018]


In this study, we examine a means for developing near-IR fluorescent sensors through streamlined, site-specific coupling with peptide-based receptors. As the penultimate step of solid-phase synthesis of a peptide-based receptor, we show a simple means of labeling the N’ terminus with the near IR fluorophore IR-783 to afford a viable fluorescent sensor after cleavage from the resin. The proof-of-concept probe utilized a biotin mimetic peptide sequence as the receptive moiety. Here we revealed a “turn-on” fluorescence enhancement upon binding of the biotin mimetic probe to its intended streptavidin target. Not all peptide-receptive moieties tested were able to generate such an enhancement upon target binding, and as such, the rationale for the observed fluorescence response properties is discussed. View Full-Text
Keywords: peptide; probe; fluorescence; sensor peptide; probe; fluorescence; sensor

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Kwak, E.-A.; Kydd, L.; Lim, B.; Jaworski, J. IR-783 Labeling of a Peptide Receptor for ‘Turn-On’ Fluorescence Based Sensing. Chemosensors 2018, 6, 47.

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