Aptamer Technologies

A special issue of Chemosensors (ISSN 2227-9040).

Deadline for manuscript submissions: closed (30 September 2018) | Viewed by 10877

Special Issue Editors

Department of Chemistry, Loughborough University, Loughborough LE11 3TU, UK
Interests: electroanalytical chemistry; sensors; nanopore; resistive pulse sensors; aptamers; nanoparticles; fluidics; magnetic nanoparticles; liquid; liquid electrochemistry; emulsion reactors; colloidal characterisation

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Guest Editor
Loughborough University, Loughborough, Loughborough LE11 3TU, UK
Interests: rapid/early diagnosis of infectious and chronic diseases; food, environmental and biological safety; security and monitoring; characterization, sensing and control systems in bio-manufacturing

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Guest Editor
Aptamer Group Ltd, Suite 2.80 – 2.87, Bio Centre, Innovation Way, Heslington, York, UK
Interests: novel application of aptamer technologies; aptamer based diagnostics; biosensor development; aptamer based purification reagents; small molecule detection; disease stratification and monitoring; environmental monitoring

Special Issue Information

Dear Colleagues,

The continued advancement of aptamer-based technologies, and the pursuits of industry and academia to deliver new aptamers more easily, and with enhanced properties, has enriched a range of applications, such as therapeutics, bioimaging, and environmental purification and monitoring. One such area that continues to grow is that of aptamer-based sensors. The chemical versatility of synthesized aptamers allows for modified bases or amino acids to be incorporated into their secondary structures.Many sensing applications utilize chemical modifications in combination with the change in tertiary structures to create structure-switching sensors, polymerase chain reaction, and electrochemical and optical assays.

The term aptamer encompasses DNA, RNA and peptide oligomers, each subset with its own unique set of chemical properties, stabilities, and applications. In this Special Issue, we want to highlight the recent and exciting developments in the field of aptamer research. The emphasis will be on the ways that these technologies have enabled new measurements, chemical processes, and methodologies. Examples of topics will include biosensors, security, food safety, antimicrobial resistance, flow technologies, such as lateral flow and functionalized flow reactors, point of care diagnostics, and more fundamental research into the rapid identification and modification of peptides with additional properties, such as DNAzymes or enhanced affinity, to name a few.  

Dr. Mark Platt
Dr. Sourav Ghosh
Dr. David Bunka
Guest Editors

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Keywords

  • AptaSensors
  • Biosensors
  • DNAzymes
  • Phage Display
  • Peptide Aptamers
  • Riboswitches
  • Food Security
  • Tagless Detection
  • Electrochemical Sensors
  • Optical Sensors
  • PCR
  • Environmental Sensors
  • Multiplexed Assay
  • Antimicrobial Resistance

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Published Papers (2 papers)

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Research

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11 pages, 1131 KiB  
Communication
IR-783 Labeling of a Peptide Receptor for ‘Turn-On’ Fluorescence Based Sensing
by Eun-A Kwak, LeNaiya Kydd, Butaek Lim and Justyn Jaworski
Chemosensors 2018, 6(4), 47; https://doi.org/10.3390/chemosensors6040047 - 16 Oct 2018
Cited by 6 | Viewed by 5217
Abstract
In this study, we examine a means for developing near-IR fluorescent sensors through streamlined, site-specific coupling with peptide-based receptors. As the penultimate step of solid-phase synthesis of a peptide-based receptor, we show a simple means of labeling the N’ terminus with the near [...] Read more.
In this study, we examine a means for developing near-IR fluorescent sensors through streamlined, site-specific coupling with peptide-based receptors. As the penultimate step of solid-phase synthesis of a peptide-based receptor, we show a simple means of labeling the N’ terminus with the near IR fluorophore IR-783 to afford a viable fluorescent sensor after cleavage from the resin. The proof-of-concept probe utilized a biotin mimetic peptide sequence as the receptive moiety. Here we revealed a “turn-on” fluorescence enhancement upon binding of the biotin mimetic probe to its intended streptavidin target. Not all peptide-receptive moieties tested were able to generate such an enhancement upon target binding, and as such, the rationale for the observed fluorescence response properties is discussed. Full article
(This article belongs to the Special Issue Aptamer Technologies)
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Review

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14 pages, 10311 KiB  
Review
Modelling and Development of Electrical Aptasensors: A Short Review
by Rosella Cataldo, Maria Leuzzi and Eleonora Alfinito
Chemosensors 2018, 6(2), 20; https://doi.org/10.3390/chemosensors6020020 - 27 Apr 2018
Cited by 13 | Viewed by 4869
Abstract
Aptamers are strands of DNA or RNA molecules, chemically synthetized and able to bind a wide range of targets, from small molecules to live cells, and even tissues, with high affinity and specificity. Due to their efficient targeting ability, they have many different [...] Read more.
Aptamers are strands of DNA or RNA molecules, chemically synthetized and able to bind a wide range of targets, from small molecules to live cells, and even tissues, with high affinity and specificity. Due to their efficient targeting ability, they have many different kinds of applications. Particularly attractive is their use in biotechnology and disease therapy, in substitution of antibodies. They represent a promising way for early diagnosis (aptasensors), but also for delivering imaging agents and drugs and for inhibiting specific proteins (therapeutic aptamers). Starting by briefly reviewing the most recent literature concerning advances in biomedical applications of aptamers and aptasensors, the focus is on the issues of a theoretical/computational framework (proteotronics) for modelling the electrical properties of biomolecules. Some recent results of proteotronics concerning the electrical, topological and affinity properties of aptamers are reviewed. Full article
(This article belongs to the Special Issue Aptamer Technologies)
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